Utilization of Andong Leaf Extract (Cordyline fruticosa) as an Alternative for Coloring the Eggs of Intestinal Nematode

Microscopic examination is essential to find the type of worm that causes infection. Direct examination of worm eggs needs to use staining to distinguish between worm eggs and food residues that are not completely digested. The dyes commonly used are synthetic. The purpose of this study was to replace synthetic dyes with natural dyes with Andong (Cordyline Fruticosa) leaf juice. It can be used as an alternative or a substitute for dyes. This research method is descriptive, conducted at the Parasitology Laboratory of Poltekkes Kemenkes Pontianak. The population in this study was the juice of the leaves of Andong (Cordyline Fruticosa). The method of inspection is direct preparation with an object glass as is routinely done, but the dye is replaced with Andong leaf juice (Cordyline Fruticosa). The results showed that natural dye Andong leaf juice (Cordyline Fruticosa), gave a clear color to the absorption of intestinal nematode worm eggs as the test target. This study concludes that the juice of the leaves of Andong (Cordyline Fruticosa) with alcohol solvent can be used as an alternative dye for the examination of intestinal nematode worm eggs.

Effect of Carica papaya Leaf Juice on Blood Cell Count of Busulfan-Induced Chronic Bone Marrow Aplasia in Mice

Introduction: Aplastic anemia is a syndrome of bone marrow failure comprising of peripheral pancytopenia and marrow hypoplasia. Recently, research has shown papaya leaf juice to increase blood cells counts. Aims & Objectives: This research was planned to investigate the effect of papaya leaf juice on blood cell counts in busulfan induced myelosuppressed mice. Place and duration of study: Post Graduate Medical Institute, Lahore from September 2015 to December 2015. Material &Methods: This animal experimental study comprised of 36 adult healthy Swiss albino mice weighing 30-35 g, divided randomly into three equal groups. Busulfan was given orally (14mg/kg) to induce myelosuppression to busulfan group and Carica papaya+ busulfan group on 8 occasions over a period of 23 days. Carica papaya leaf juice (10ml/kg) was given to the Carica papaya+ busulfan group daily for 23 days to observe its role in preventing myelosuppression. Normal group was given distilled water in an equivalent amount. One day after the final dose of busulfan and Carica papaya, the animals were sampled for blood cell counts. Data was analyzed using SPSS version 20. A p-value < 0.05 and below was considered as significant. Results: The Carica papaya leaf juice not only prevented significant fall in hemoglobin (10.86 ± 1.455 g/dl), RBC (9.54 ± 0.954× 106/mm3) and platelet count (958.92 ± 176.22 x103/mm3) vs (9.70 ± 0.904,11.24 ± 0.952 g/dl), RBC count (6.5025 ± 1.43,10.43 ± 0.234× 106/mm3) and platelet count (627.83 ± 151.63, 3881.08 ± 83.13 x103/mm3) in busulfan group but also kept them close to normal range. Total leukocyte count in C.papaya treated group improved significantly to 8116.67± 1540.85/mm3 vs (4516.67±789.51, 6666.67±798.10/mm3) busulfan treated and normal groups respectively. Conclusion: The Carica papaya leaf juice possesses significant myeloprotective activity. It may be a good candidate for prevention of chemotherapy induced pancytopenia

Wound healing potential of Launaea pinnatiϔida Cass leaf juice in rats

Launaea pinnatifida is a procumbent herb native to the coastal area and found throughout India and many other countries. It is utilized as a dietary plant in the preparation of various dishes in the Maldives. Traditionally it is used for the treatment of skin injuries and is reported to contain flavonoids, steroids, glycosides, tannins, saponins etc. As no scientific work has been found to be published related to wound healing potential of the L. pinnatifida plant, the present study investigated the effects of dried leaf juice of L. pinnatifida on wound healing property in rats using various wound models. In result analysis, the wound contraction percentage was found to be increased and the scar area and epithelization time were found to be decreased when 3 % ointment L. pinnatifida leaf juice was applied topically. Similarly, with incision and dead space wound models, wound breaking strength and hydroxyproline levels were found to be increased. So, Launaea pinnatifida leaf juice showed significant wound healing activity in all wound models, which supports its claim for being used traditionally in skin diseases.

Study the Effect of Ipomea carnea Jacq. Patra swarasa (Leaves Juice) in Treatment of Indralupta (Alopecia Areata): A Pilot Study

Introduction: Hair and skin disease create negative impact on individuals. Although such diseases doesn’t have any life threatening effects like systemic diseases still have much more importance as the cosmetic issue concern. Indralupta is a rare entity found in both male, and female at any age which can be correlated with Alopecia areata. There are many treatment modalities are available for the Indralupta (Alopecia areata) but application of leaf juice of Ipomoea carnia is simple and cost effective treatment. Aim &Objective: Study the effect of Ipomea carnea Jacq. patra swarasa (leaves juice) in management of Indralupta (Alopecia Areata). To study the adverse effects of Ipomea carnia leaf juice local application if noted during study. Methodology: Fresh leaf juice will be extracted from clean leaf of Ipomea carnia and apply over the spot where hairs are lost for 15 days at morning before bath. 10 objects will be selected from kaychikitsa OPD randomly and consider for study after their willingness and consent. Outcomes will be assessed with the help of different criteria according to hair texture, Hair fall and scalp area examination. Result: Result will be observed according to criteria and Wilcoxon signed rank test will be a applied. Conclusion: Ipomea carnea will be effective in Indralupta(Alopecia areata).

Evaluation of medicinal uses, phytochemistry and biological activities of Ehretia cymosa Thonn. (Ehretiaceae)

Ehretia cymosa Thonn. is a deciduous medium-sized to large tree which occurs naturally from Sierra Leone in West Africa to Eritrea and Kenya in East Africa, and Zimbabwe in southern Africa. This study aims to provide a comprehensive review of medicinal uses, phytochemistry and biological activities of E. cymosa. This review examines the existing literature on the medicinal uses, phytochemistry and biological activities of E. cymosa. This study revealed that the bark, leaf juice, leaves, roots, seeds, stems, twigs and whole plant parts of E. cymosa are mainly used as aphrodisiac, laxative and ethnoveterinary medicines and as traditional medicines for gastro-intestinal problems, wounds, malaria, fever, typhoid, convulsions, epilepsy, toothache and respiratory infections. Phytochemical research revealed that the species is characterized by alkaloids, anthraquinones, essential oils, fatty acids, flavonoids, glycosides, phenolics, proanthocyanidins, pseudotannins, reducing sugars, saponins, steroids, tannins and terpenes. Ethnopharmacological research revealed that the extracts of E. cymosa and phytochemical compounds isolated from the species showed antibacterial, antidiabetic, antihyperglycaemic and antioxidant activities. Ehretia cymosa should be subjected to detailed phytochemical, pharmacological and toxicological evaluations aimed at correlating its medicinal uses with its phytochemistry and pharmacological properties.

Pendampingan Budidaya dan Pengolahan Nutrasetikal Daun Bangun-bangun Untuk Ibu Rumah Tangga

Warga dawis Arbey RT 04 RW 04 Kelurahan Sampangan Semarang sebagian besar adalah ibu rumah tangga usia produktif. Lingkungan di daerah ini masih belum dimanfaatkan secara optimal sehingga mendorong pentingnya didirikan kelompok usaha yang memanfaatkan pekarangan rumah. untuk budidaya tanaman obat. Salah satu contoh TOGA yang mudah ditanam adalah daun bangun-bangun (Coleus amboinicus). Daun bangun-bangun berkhasiat sebagai pelancar ASI dengan dibuat menjadi jus siap minum. Pengembangan produk jus daun bangun-bangun perlu didukung oleh sarana prasarana, organisasi, SDM (Sumber Daya Manusia) dan pemasaran. Kegiatan pengabdian ini bertujuan untuk memberikan ketrampilan pada warga membudidayakan, mengolah produk daun bangun-bangun dan memasarkannya. Terdapat 2 metode yang digunakan yaitu penyuluhan dan praktik langsung. Materi penyuluhan adalah cara budidaya, pengolahan dan pemasaran jus daun bangun-bangun. Metode kedua adalah praktik budidaya dan pembuatan jus daun bangun-bangun. Hasil dari kegiatan pengabdian ini yaitu 15 orang peserta berhasil membudidayakan tanaman bangun-bangun.Most of the residents of dawis Arbey RT 04 RW 04 Kelurahan Sampangan Semarang are housewives of productive age. The environment in this area is still not optimally utilized, thus encouraging the importance of establishing business groups that utilize house yards. for the cultivation of medicinal plants. One example of TOGA that is easy to plant is the leaves of bangun-bangun (Coleus amboinicus). The leaves of the wakes have the effect of facilitating breast milk by being made into ready-to-drink juice. The development of leaf juice products needs to be supported by infrastructure, organization, human resources (human resources) and marketing. This service activity aims to provide skills to residents to cultivate, process leaf-building products and market them. There are 2 methods used, namely counseling and direct practice. The extension material is the method of cultivating, processing and marketing the juice from the leaves. The second method is the practice of cultivating and making leaf juice. The result of this service activity was that 15 participants succeeded in cultivating bangun-bangun.

Effects of Xanthan gum on rheological properties of Aloe vera-Moringa leaf juice blends

Understanding the impacts of hydrocolloid agents on the rheological properties of nutraceutical beverages like Aloe vera-moringa juice blend is very useful for functional properties and product stability. To this end, the effects of xanthan gum on rheological properties in Aloe vera-moringa leaf juice blends were investigated using a Brookfield rheometer. Aloe vera-moringa leaf juice blends were prepared and then incorporated with xanthan gum at different ratios (w/w) of 0.2%, 0.4%, 0.6%, 0.8%, and 1%. The results revealed that the viscosity of Aloe vera-moringa juice blend was strongly affected by the xanthan gum addition. The viscosity profiles depicted a decreasing trend in viscosity when a shear rate increased. The Aloe vera-moringa leaf juice blend changes from a Newtonian fluid to the non-Newtonian fluid as the xanthan gum fraction increases. Moreover, shear stress was observed to increase with increasing in xanthan gum concentrations. For quantitative analysis, both Power-law and Bingham model equations were fitted to experimental data to easily describe the flow behaviour of the Aloe vera-moringa leaf juice blends. The juices added with xanthan gum of 0.4% to 1% were found to show a shear-thinning behaviour, since the flow behaviour index, n < 1. This implies that the apparent viscosity decreases as the shear rate increases. Keywords: Aloe vera juice, Moringa leaf juice, Xanthan gum, Rheological property, Nutraceutical beverage

Immunomodulatory Activities of Carica papaya L. Leaf Juice in a Non-Lethal, Symptomatic Dengue Mouse Model

The role of Carica papaya L. leaf juice in immune dysregulation caused by dengue virus infection remains unclear. This study aimed to investigate the immunomodulatory activities of the freeze-dried C. papaya leaf juice (FCPLJ) on AG129 mice infected with a clinical DENV-2 (DMOF015) isolate. The infected AG129 mice were orally treated with 500 and 1000 mg/kg/day of FCPLJ, for three days. Platelet, leukocyte, lymphocyte and neutrophil counts were microscopically determined. The level of plasma proinflammatory cytokines was measured by multiplex immunoassay. The levels of intracellular cytokines and viral RNA were determined by RT-qPCR technique. The results showed that the FCPLJ treatment increased the total white blood cell and neutrophil counts in the infected mice. The FCPLJ treatment decreased the level of GM-CSF, GRO-alpha, IL-1 beta, IL-6, MCP-1 and MIP-1 beta in the plasma of the infected mice. The intracellular IL-6 and viral RNA levels in the liver of infected mice were decreased by the FCPLJ treatment. In conclusion, this study supports the potential immunomodulatory role of the FCPLJ in a non-lethal, symptomatic dengue mouse model. Further studies on the action mechanism of the C. papaya leaf juice and its possible use as adjunctive dengue immunotherapy are warranted.

Kadar Kolesterol, Asam Urat dan Glukosa Darah Ayam Petelur yang Diberi Jus Daun Sirih (Piper betle Linn) pada Level yang Berbeda

ABSTRACT This study aims to assess cholesterol, uric acid and blood glucose levels in laying hens which are fed with the addition of betel leaf juice (Piper Bettle Linn). There were 16 chickens used in layer phase hens. The cage used is an individual cage equipped with a place for feeding and drinking water. The treatments in this study consisted of: (P0) commercial feed + 0 ml of betel leaf juice, (P1) commercial feed + 5 ml of betel leaf juice, (P2) commercial feed + 10 ml of betel leaf juice, and (P3) commercial feed + 15 ml of betel leaf juice. The variables observed were cholesterol, uric acid and blood glucose levels. The data obtained from the research results were analyzed using variance and continued with Duncan's multiple range test. The results showed that the administration of betel leaf juice had no significant effect (p <0.05) on cholesterol, uric acid and blood glucose levels in layer phase hens. Based on the results of the study, it can be concluded that the administration of betel leaf juice does not affect the amount of cholesterol, uric acid, and glucose levels in layer phase hens, but these conditions are still in normal physiological conditions. Keywords: uric acid, layer hens, glucose, cholesterol ABSTRAK Penelitian ini bertujuan untuk mengkaji tentang kadar kolesterol, asam urat dan glukosa darah ayam petelur yang diberi penambahan jus daun sirih (Piper betle Linn) pada level yang berbeda. Ayam yang digunakan sebanyak 16 ekor induk ayam petelur betina fase layer. Kandang yang digunakan yaitu kandang individu yang dilengkapi dengan tempat pakan dan air minum. Perlakuan pada penelitian ini terdiri atas: (P0) pakan basal + 0 ml jus daun sirih, (P1) pakan basal + 5 ml jus daun sirih, (P2) pakan basal + 10 ml jus daun sirih, dan (P3) pakan basal + 15 ml jus daun sirih. Variabel yang diamati adalah kadar kolesterol, asam urat, dan glukosa darah. Data yang diperoleh dari hasil penelitian dianalisis menggunakan sidik ragam dan dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa pemberian jus daun sirih tidak memberikan pengaruh yang nyata (p<0.05) terhdadap kadar kolesterol, asam urat dan glukosa darah ayam petelur fase layer. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian jus daun sirih tidak mempengaruhi jumlah kadar kolestero, asam urat, dan glukosa ayam petelur fase layer, namun kondisi tersebut masih dalam kondisi fisiologis normal. Kata Kunci : asam urat, ayam petelur, glukosa, kolesterol