Scanning and transmission electron microscopy of an unusual soil bacterium: application of the critical point drying method

Micron (1969) ◽  
1971 ◽  
Vol 3 (4) ◽  
pp. 474-485
Author(s):  
V. Bystricky ◽  
H.G. Fromme ◽  
M. Pfautsch ◽  
G. Pfefferkorn
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Critical Point ◽  
Soil Bacterium ◽  
Drying Method ◽  
Transmission Electron ◽  
Critical Point Drying

Scanning and Transmission Electron Microscopy of Human Lung in a Patient with Sarcoid-like Reaction

1974 ◽  
Vol 32 ◽  
pp. 14-15
Author(s):  
John H. L. Watson ◽  
Jessica Goodwin ◽  
E. Osborne Coates
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Hypersensitivity Reaction ◽  
Light Microscopy ◽  
Critical Point ◽  
Clinical Evidence ◽  
Human Lung ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Transmission Electron

Biopsies of lung were taken at operation from a patient with semi-acute diffuse pulmonary infiltrates for study by TEM and SEM. Tissue by light microscopy showed non-caseating granulomas consistent with sarcoidosis. Clinical evidence suggested a hypersensitivity reaction related to inhalation of substance of undetermined nature. Samples were fixed in glutaraldehyde, cacodylate-buffered. They were critical point dried and coated with Au-Pd for SEM, and were handled appropriately for TEM in Araldite. Sections were contrasted with uranyl acetate and lead citrate.

Li EXCESS Li4+xTi5-xO12-δ/C COMPOSITE USING SPRAY-DRYING METHOD AND ITS ELECTRODE PROPERTIES

2012 ◽  
Vol 05 (01) ◽  
pp. 1250001 ◽  
Author(s):  
DAISUKE YOSHIKAWA ◽  
NORIO SUZUKI ◽  
YOSHIHIRO KADOMA ◽  
KOICHI UI ◽  
NAOAKI KUMAGAI
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Spray Drying ◽  
Carbon Composite ◽  
Residual Carbon ◽  
Drying Method ◽  
Electrode Performance ◽  
Transmission Electron ◽  
Carbon Composite Material ◽  
Prepared Material

We have prepared a lithium excess carbon composite material, Li 4+x Ti 5-x O 12-δ/ C (LTO/C), using various amounts of sucrose as a carbon source by the spray-drying method. The prepared materials were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), and elemental analysis. The prepared material had the Li4Ti5O12 phase including 3.9–18.4 wt.% carbon. Transmission electron microscopy images and the selected area diffraction (SAD) pattern showed that the prepared materials consisted of a carbon nanonetwork in the LTO/C composite. The charge–discharge cycling tests were carried out using the R2032 coin-type cell under the following conditions; 1.2–3.0 V, 0.1 C–10 C (1 C = 175 mA g-1), 25°C. Based on the electrochemical results, the electrode performance of the prepared material was improved with increasing amounts of residual carbon, in particular, LTO/C including 6.2 wt.% residual carbon exhibited the best electrode performance of 156 mAh g-1 at 1 C during 50 cyclings when compared to the other materials.

A Preliminary Study of Thin Bird Disease

1981 ◽  
Vol 39 ◽  
pp. 602-603
Author(s):  
Ronald W. Davis ◽  
Samual Kenzy ◽  
Erik H. Stauber
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Weight Loss ◽  
Light Microscopy ◽  
Critical Point ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Transmission Electron ◽  
Half Strength ◽  
Preliminary Study

We have initiated a study of a disease that is usually fatal to domestic parakeets and canaries. We have called this syndrome “thin bird disease” for lack of a better name.Clinically, the disease is diagnosed after observing a bird that is thin, exhibits severe weight loss, lethargy, and ruffled feathers. Fecal smears of affected birds contain many very large, single-celled, rod-shaped bacteria measuring up to 1μm x 90μm.Post mortem examination of birds exhibiting external symptoms of the disease reveals an enlarged ventriculus (gizzard) that contains unmacerated seeds. A jelly-like mass is found in the ventriculus and pro-ventriculus of affected birds. This material contains bacteria morphologically identical to those found in fecal smears (Figs. 3, 4).Samples of ventriculus and pro-ventriculus from birds clinically diagnosed as diseased and normal were collected and processed for light microscopy, scanning and transmission electron microscopy. The specimens were fixed in half strength Karnovsky's fixative, post fixed in OsO4, and either embedded in plastic or critical point dried.

Rapid Chemical Dehydration of Mammalian Tissues for Scanning Electron Microscopy using 2 ,2-Dimethoxypropane

1976 ◽  
Vol 34 ◽  
pp. 340-341
Author(s):  
Morton D Maser ◽  
John J. Trimble
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Laboratory Mouse ◽  
Transmission Electron ◽  
Critical Point Drying ◽  
Mammalian Tissues ◽  
Cervical Dislocation ◽  
Structural Preservation ◽  
Scanning Electron

Muller and Jacks used 2,2-dimethoxypropane (DMP) as a dehydrating agent for biological specimens prior to embedment, ultrathin sectioning, and examination by transmission electron microscopy. Acidified DMP reacts endothermically with water to produce methanol and acetone. Muller and Jacks demonstrated excellent fine structural preservation in a variety of specimens dehydrated with DMP.We have used the same method, prior to critical point drying, to prepare specimens for scanning electron microscopy (SEM) . Portions of the trachea, small intestine, and kidney from a laboratory mouse killed by cervical dislocation were fixed for 16 hours in cold 3% glutaraldehyde in 0.1 M phosphate buffer, pH 7.2.

Electron microscopy of T1-bacteriophage adsorbed to clay minerals: application of the critical point drying method

1975 ◽  
Vol 21 (8) ◽  
pp. 1278-1282 ◽  
Author(s):  
Vojtech Bystricky ◽  
G. Stotzky ◽  
Milton Schiffenbauer
Keyword(s):  
Electron Microscopy ◽  
Critical Point ◽  
Clay Minerals ◽  
Surface Interaction ◽  
Drying Method ◽  
Critical Point Drying

A T1-bacteriophage appears to be strongly adsorbed by clay minerals. The critical point drying method has been used to facilitate visualization of this surface interaction by electron microscopy.

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