Rapid Chemical Dehydration of Mammalian Tissues for Scanning Electron Microscopy using 2 ,2-Dimethoxypropane
1976 ◽
Vol 34
◽
pp. 340-341
Keyword(s):
Muller and Jacks used 2,2-dimethoxypropane (DMP) as a dehydrating agent for biological specimens prior to embedment, ultrathin sectioning, and examination by transmission electron microscopy. Acidified DMP reacts endothermically with water to produce methanol and acetone. Muller and Jacks demonstrated excellent fine structural preservation in a variety of specimens dehydrated with DMP.We have used the same method, prior to critical point drying, to prepare specimens for scanning electron microscopy (SEM) . Portions of the trachea, small intestine, and kidney from a laboratory mouse killed by cervical dislocation were fixed for 16 hours in cold 3% glutaraldehyde in 0.1 M phosphate buffer, pH 7.2.
1978 ◽
Vol 36
(2)
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pp. 82-83
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1972 ◽
Vol 30
◽
pp. 150-151
1976 ◽
Vol 34
◽
pp. 444-445
1996 ◽
Vol 54
◽
pp. 602-603
2017 ◽
2021 ◽
pp. 1759-1829
2012 ◽
Vol 174-177
◽
pp. 508-511
1981 ◽
Vol 26
(2)
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pp. 103-114
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