Electron microscopy of T1-bacteriophage adsorbed to clay minerals: application of the critical point drying method

1975 ◽  
Vol 21 (8) ◽  
pp. 1278-1282 ◽  
Author(s):  
Vojtech Bystricky ◽  
G. Stotzky ◽  
Milton Schiffenbauer
Keyword(s):  
Electron Microscopy ◽  
Critical Point ◽  
Clay Minerals ◽  
Surface Interaction ◽  
Drying Method ◽  
Critical Point Drying

A T1-bacteriophage appears to be strongly adsorbed by clay minerals. The critical point drying method has been used to facilitate visualization of this surface interaction by electron microscopy.

The Critical Point Drying Method Applied to Scanning Electron Microscopy of L-929 Cells

1970 ◽  
Vol 28 ◽  
pp. 278-279
Author(s):  
Charles TurnbiLL ◽  
Delbert E. Philpott
Keyword(s):  
Electron Microscopy ◽  
Carbon Dioxide ◽  
Surface Tension ◽  
Critical Point ◽  
Freeze Drying ◽  
Attractive Alternative ◽  
Crystal Formation ◽  
Critical Point Drying ◽  
Time Required ◽  
Scanning Electron

The advent of the scanning electron microscope (SCEM) has renewed interest in preparing specimens by avoiding the forces of surface tension. The present method of freeze drying by Boyde and Barger (1969) and Small and Marszalek (1969) does prevent surface tension but ice crystal formation and time required for pumping out the specimen to dryness has discouraged us. We believe an attractive alternative to freeze drying is the critical point method originated by Anderson (1951; for electron microscopy. He avoided surface tension effects during drying by first exchanging the specimen water with alcohol, amy L acetate and then with carbon dioxide. He then selected a specific temperature (36.5°C) and pressure (72 Atm.) at which carbon dioxide would pass from the liquid to the gaseous phase without the effect of surface tension This combination of temperature and, pressure is known as the "critical point" of the Liquid.

Preservation of Plant Cell Surfaces For Scanning Electron Microscopy

1973 ◽  
Vol 31 ◽  
pp. 472-473
Author(s):  
Linda M. Sicko ◽  
Thomas E. Jensen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Critical Point ◽  
Filter Paper ◽  
Freeze Drying ◽  
Cell Surfaces ◽  
Air Drying ◽  
Popular Method ◽  
Critical Point Drying ◽  
Scanning Electron

The use of critical point drying is rapidly becoming a popular method of preparing biological samples for scanning electron microscopy. The procedure is rapid, and produces consistent results with a variety of samples. The preservation of surface details is much greater than that of air drying, and the procedure is less complicated than that of freeze drying. This paper will present results comparing conventional air-drying of plant specimens to critical point drying, both of fixed and unfixed material. The preservation of delicate structures which are easily damaged in processing and the use of filter paper as a vehicle for drying will be discussed.

scholarly journals Critical Point Drying: An Effective Drying Method for Direct Measurement of the Surface Area of a Pretreated Cellulosic Biomass

Polymers ◽  
2018 ◽  
Vol 10 (6) ◽  
pp. 676 ◽  
Author(s):  
Kyu-Young Kang ◽  
Kyung-Ran Hwang ◽  
Ji-Yeon Park ◽  
Joon-Pyo Lee ◽  
Jun-Seok Kim ◽  
...  
Keyword(s):  
Critical Point ◽  
Surface Area ◽  
Direct Measurement ◽  
Cellulosic Biomass ◽  
Drying Method ◽  
Critical Point Drying
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