The activity of cell-wall degrading enzymes in tomato roots infected with Pyrenochaeta lycopersici and the effect of sugar concentrations in these roots on disease development

1976 ◽  
Vol 9 (3) ◽  
pp. 313-320 ◽  
Author(s):  
P.W. Goodenough ◽  
R.J. Kempton
2006 ◽  
Vol 84 (9) ◽  
pp. 1395-1404 ◽  
Author(s):  
Inmaculada Jorge ◽  
Juan A. Navas-Cortés ◽  
Rafael M. Jiménez-Díaz ◽  
Manuel Tena

Production of cell wall degrading enzymes (CWDEs) polygalacturonase (PG), pectate lyase (PL), and xylanase was studied in chickpeas ( Cicer arietinum L. ‘P-2245’) inoculated with Fusarium oxysporum f. sp. ciceris (Padwick) Matuo & K. Sato races 0 (mildly virulent, causing a yellowing syndrome) and 5 (highly virulent, causing a wilting syndrome) by the water-culture method. These CWDEs were similarly produced in both syndromes. PG and PL were the only enzymes occurring in roots and stems and attained the highest specific activity, this being generally higher for race 5 than for race 0. Gel filtration chromatography revealed a similar complement of in planta expressed pectinase isoforms, dominated by an endo-PG and two endo-PLs, the endo-PLs being differentially expressed by the two races. CWDE activities in roots and stems were positively correlated with development of yellowing and wilting. Exceptions to this were PG in stems, which was negatively correlated with the development of yellowing, and PG in roots, which showed a negative trend with development of either syndrome. The levels of CWDEs that significantly correlated with disease development were adequately described by exponential functions of disease progress. Results have implications for the role played by CWDEs in the early and later stages of pathogenesis in chickpea fusarium wilt.


2019 ◽  
Vol 377 ◽  
pp. 120325 ◽  
Author(s):  
Antonio Zuorro ◽  
Veronica Malavasi ◽  
Giacomo Cao ◽  
Roberto Lavecchia

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