Long-term semi-continuous production of recombinant baculovirus protein in a repeated (fed-)batch two-stage reactor system

1996 ◽  
Vol 18 (6) ◽  
pp. 460-466 ◽  
Author(s):  
Frank L.J. van Lier ◽  
Johannes P.T.W. van den Hombergh ◽  
Cornelis D. de Gooijer ◽  
Mirjam M. den Boer ◽  
Just M. Vlak ◽  
...  
2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Ruijuan Ma ◽  
Zhen Zhang ◽  
Zhuzhen Tang ◽  
Shih-Hsin Ho ◽  
Xinguo Shi ◽  
...  

AbstractCo-production of multiple compounds is an efficient approach to enhance the economic feasibility of microalgae-based metabolites production. In this study, Chlorella sorokiniana FZU60 was cultivated under different bioprocess strategies to enhance the co-production of lutein and protein. Results showed that both lutein and protein content (7.72 and 538.06 mg/g, respectively) were highest at the onset of nitrogen deficiency under batch cultivation. Semi-batch III strategy, with 75% microalgal culture replacement by fresh medium, obtained similar content, productivity, and yield of lutein and protein as batch cultivation, demonstrating that it can be used for stable and continuous production. Fed-batch II strategy, feeding with 1/3 modified BG11 medium, achieved super-high lutein and protein yield (28.81 and 1592.77 mg/L, respectively), thus can be used for high-output production. Besides, two-stage strategy, combining light intensity shift and semi-batch cultivation, gained extremely high lutein and protein productivity (15.31 and 1080.41 mg/L/day, respectively), thereby is a good option for high-efficiency production. Moreover, the fed-batch II and two-stage strategy achieved high-quality lutein and protein, thus are promising for the co-production of lutein and protein in C. sorokiniana FZU60 for commercial application.


BMJ Open ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. e044219
Author(s):  
J X Harmeling ◽  
Kevin Peter Cinca ◽  
Eleni-Rosalina Andrinopoulou ◽  
Eveline M L Corten ◽  
M A Mureau

IntroductionTwo-stage implant-based breast reconstruction is the most commonly performed postmastectomy reconstructive technique. During the first stage, a tissue expander creates a sufficiently large pocket for the definite breast implant placed in the second stage. Capsular contracture is a common long-term complication associated with implant-based breast reconstruction, causing functional complaints and often requiring reoperation. The exact aetiology is still unknown, but a relationship between the outer surface of the implant and the probability of developing capsular contracture has been suggested. The purpose of this study is to determine whether polyurethane-covered implants result in a different capsular contracture rate than textured implants.Methods and analysisThe Textured Implants versus Polyurethane-covered Implants (TIPI) trial is a multicentre randomised controlled trial with a 1:1 allocation rate and a follow-up of 10 years. A total of 321 breasts of female adults undergoing a two-stage breast reconstruction will be enrolled. The primary outcome is capsular contracture at 10-year follow-up which is graded with the modified Baker classification. It is analysed with survival analysis using a frailty model for clustered interval-censored data, with both an intention-to-treat and per-protocol approach. Secondary outcomes are other complication rates, surgical revision rate, patient satisfaction and quality of life and user-friendliness. Outcomes are measured 2 weeks, 6 months, 1, 2, 3, 5 and 10 years postoperatively. Interim analysis is performed when 1-year, 3-year and 5-year follow-up is completed.Ethics and disseminationThe trial has been reviewed and approved by the Medical Research Ethics Committee of the Erasmus MC, University Medical Centre Rotterdam (MEC-2018-126) and locally by each participating centre. Written informed consent will be obtained from each study participant. The results will be disseminated by publication in peer-reviewed journals.Trial registrationNTR7265.


2010 ◽  
Vol 22 (2) ◽  
pp. 230-236 ◽  
Author(s):  
Rohan Jain ◽  
Ashish Pathak ◽  
T.R. Sreekrishnan ◽  
M.G. Dastidar

2021 ◽  
Author(s):  
Pawan Agrawal ◽  
Sharifa Yousif ◽  
Ahmed Shokry ◽  
Talha Saqib ◽  
Osama Keshtta ◽  
...  

Abstract In a giant offshore UAE carbonate oil field, challenges related to advanced maturity, presence of a huge gas-cap and reservoir heterogeneities have impacted production performance. More than 30% of oil producers are closed due to gas front advance and this percentage is increasing with time. The viability of future developments is highly impacted by lower completion design and ways to limit gas breakthrough. Autonomous inflow-control devices (AICD's) are seen as a viable lower completion method to mitigate gas production while allowing oil production, but their effect on pressure drawdown must be carefully accounted for, in a context of particularly high export pressure. A first AICD completion was tested in 2020, after a careful selection amongst high-GOR wells and a diagnosis of underlying gas production mechanisms. The selected pilot is an open-hole horizontal drain closed due to high GOR. Its production profile was investigated through a baseline production log. Several AICD designs were simulated using a nodal analysis model to account for the export pressure. Reservoir simulation was used to evaluate the long-term performance of short-listed scenarios. The integrated process involved all disciplines, from geology, reservoir engineering, petrophysics, to petroleum and completion engineering. In the finally selected design, only the high-permeability heel part of the horizontal drain was covered by AICDs, whereas the rest was completed with pre-perforated liner intervals, separated with swell packers. It was considered that a balance between gas isolation and pressure draw-down reduction had to be found to ensure production viability for such pilot evaluation. Subsequent to the re-completion, the well could be produced at low GOR, and a second production log confirmed the effectiveness of AICDs in isolating free gas production, while enhancing healthy oil production from the deeper part of the drain. Continuous production monitoring, and other flow profile surveys, will complete the evaluation of AICD effectiveness and its adaptability to evolving pressure and fluid distribution within the reservoir. Several lessons will be learnt from this first AICD pilot, particularly related to the criticality of fully integrated subsurface understanding, evaluation, and completion design studies. The use of AICD technology appears promising for retrofit solutions in high-GOR inactive strings, prolonging well life and increasing reserves. Regarding newly drilled wells, dedicated efforts are underway to associate this technology with enhanced reservoir evaluation methods, allowing to directly design the lower completion based on diagnosed reservoir heterogeneities. Reduced export pressure and artificial lift will feature in future field development phases, and offer the flexibility to extend the use of AICD's. The current technology evaluation phases are however crucial in the definition of such technology deployments and the confirmation of their long-term viability.


2015 ◽  
Vol 47 (13) ◽  
pp. 1174-1179 ◽  
Author(s):  
Angela Sobiesierski ◽  
Robert Thomas ◽  
Philip Buckle ◽  
David Barrow ◽  
Peter M. Smowton

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Bu-Chun Si ◽  
Jia-Ming Li ◽  
Zhang-Bing Zhu ◽  
Yuan-Hui Zhang ◽  
Jian-Wen Lu ◽  
...  

2019 ◽  
Vol 19 (1) ◽  
pp. 141-157 ◽  
Author(s):  
Paula Guimaraes ◽  
Ricardo P.C. Leal ◽  
Peter Wanke ◽  
Matthew Morey

Purpose This paper aims to investigate the long-term impact of shareholder activism on Brazilian listed companies. Design/methodology/approach This study uses a sample of 194 companies in 2010, 2012 and 2014 and a two-stage data envelopment analysis to generate an efficiency score based on corporate governance, ownership structure and financial characteristics of companies. In the second stage, the study applies a bootstrap truncated regression to identify whether there is a relationship between the efficiency scores and a company-level activism index. Findings The results show a negative correlation between the efficiency scores and the activism index, suggesting that activist shareholders tend to target less efficient companies. A time analysis over the period 2010-2014 does not offer evidence of impacts of activism on changes of the efficiency scores. Practical implications Activist shareholders target less efficient companies. Shareholder activism increased after regulation that facilitated shareholder voting and required greater company transparency was introduced. Originality/value The two-stage nature of the procedure used in the analysis ascertains that this result is not spurious, assuring data separability between productive resources and contextual variables. This study contributes to the scarce literature on activism in emerging markets.


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