D.9 Recognition and killing of allogenic and virus-infected lymphoid cells by channel catfish peripheral blood lymphocytes

1994 ◽  
Vol 18 ◽  
pp. S81 ◽  
Keyword(s):  
Channel Catfish ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Blood Lymphocytes

Characterization of anti-hapten antibodies generated in vitro by channel catfish peripheral blood lymphocytes

1992 ◽  
Vol 16 (2-3) ◽  
pp. 139-151 ◽  
Author(s):  
Frederik W. van Ginkel ◽  
Norman W. Miller ◽  
Craig J. Lobb ◽  
L.William Clem
Keyword(s):  
Channel Catfish ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes

scholarly journals Sezary cell morphology induced in peripheral blood lymphocytes: re- evaluation

Blood ◽  
1989 ◽  
Vol 73 (6) ◽  
pp. 1603-1607
Author(s):  
AC Chu ◽  
JF Morris
Keyword(s):  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Lymphocytes ◽  
Epidermal Cells ◽  
Lymphoid Cells ◽  
T Helper ◽  
Normal Peripheral Blood ◽  
Blood Lymphocytes ◽  
Driven System ◽  
Sézary Cells

In this study we examined the effect of mitogens and epidermal cells in inducing a Sezary cell morphology in normal peripheral blood lymphocytes. Peripheral blood mononuclear cells from six healthy volunteers were stimulated with the mitogens phytohemaglutinin and concanavalin A, and also cocultivated with human epidermal cell cultures. Incubation times with mitogens and epidermal cells were four days and stimulation of the lymphocytes by mitogens was confirmed by standard 3H-thymidine uptake. Standard transmission electron microscopy showed that in the mitogen-driven system 20% to 60% (33 +/- 15%) and in the epidermal cell-driven system 5% to 15% (8 +/- 4%) of the lymphoid cells exhibited mild to moderate indentation of the nuclei with nuclear contour indices (NCI) of 4.6 to 6.5 but no Sezary cells were observed (cells with NCI greater than 6.5 and up to 19.2). In the mitogen- stimulated preparation 2% to 5% (3 +/- 1%) of the lymphoid cells showed nuclear multilobulation resembling the cells seen in adult T cell lymphoma/leukemia. Incubation of mononuclear cells for longer periods of up to 4 weeks with mitogens and exogenous IL-2 resulted in no further morphologic changes. Using an indirect immunogold technique at the electron microscopic level, the cells showing nuclear indentation or lobulation were shown to bear both T helper (CD4) and T suppressor (CD8) cell phenotypes in a similar ratio to the total numbers of T helper and T suppressor cells present. Mitogens and epidermal cells are thus not able to induce a morphologic change to Sezary cells in normal peripheral blood lymphocytes.

scholarly journals Sezary cell morphology induced in peripheral blood lymphocytes: re- evaluation

Blood ◽  
1989 ◽  
Vol 73 (6) ◽  
pp. 1603-1607 ◽  
Author(s):  
AC Chu ◽  
JF Morris
Keyword(s):  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Lymphocytes ◽  
Epidermal Cells ◽  
Lymphoid Cells ◽  
T Helper ◽  
Normal Peripheral Blood ◽  
Blood Lymphocytes ◽  
Driven System ◽  
Sézary Cells

Abstract In this study we examined the effect of mitogens and epidermal cells in inducing a Sezary cell morphology in normal peripheral blood lymphocytes. Peripheral blood mononuclear cells from six healthy volunteers were stimulated with the mitogens phytohemaglutinin and concanavalin A, and also cocultivated with human epidermal cell cultures. Incubation times with mitogens and epidermal cells were four days and stimulation of the lymphocytes by mitogens was confirmed by standard 3H-thymidine uptake. Standard transmission electron microscopy showed that in the mitogen-driven system 20% to 60% (33 +/- 15%) and in the epidermal cell-driven system 5% to 15% (8 +/- 4%) of the lymphoid cells exhibited mild to moderate indentation of the nuclei with nuclear contour indices (NCI) of 4.6 to 6.5 but no Sezary cells were observed (cells with NCI greater than 6.5 and up to 19.2). In the mitogen- stimulated preparation 2% to 5% (3 +/- 1%) of the lymphoid cells showed nuclear multilobulation resembling the cells seen in adult T cell lymphoma/leukemia. Incubation of mononuclear cells for longer periods of up to 4 weeks with mitogens and exogenous IL-2 resulted in no further morphologic changes. Using an indirect immunogold technique at the electron microscopic level, the cells showing nuclear indentation or lobulation were shown to bear both T helper (CD4) and T suppressor (CD8) cell phenotypes in a similar ratio to the total numbers of T helper and T suppressor cells present. Mitogens and epidermal cells are thus not able to induce a morphologic change to Sezary cells in normal peripheral blood lymphocytes.

Changes in topoisomerase I activity after irradiation of lymphoid cells

1985 ◽  
Vol 5 (10-11) ◽  
pp. 907-912 ◽  
Author(s):  
Alan Johnstone ◽  
Ruth McNerney
Keyword(s):  
Peripheral Blood ◽  
Topoisomerase I ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Dna Breaks ◽  
Γ Irradiation ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Nuclear Extracts

The activity of topoisomerase I in nuclear extracts increased about three-fold 5 min after γ-irradiation (840–2500 rads) of human peripheral blood lymphocytes or cultured lymphoblastoid cells. The change may reflect modification of the enzyme by nuclear ADP-ribosyl transferase, which is known to be activated by DNA breaks.

scholarly journals ИЗУЧЕНИЕ ВЛИЯНИЯ НАНОЧАСТИЦ СЕРЕБРА IN VITRO НА УРОВЕНЬ ЭКСПРЕССИИ МОЛЕКУЛЯРНЫХ МАРКЕРОВ АКТИВАЦИИ ЛИМФОЦИТОВ И МАРКЕРА АУТОИММУННОГО ПРОЦЕССА ПЕРИФЕРИЧЕСКОЙ КРОВИ БОЛЬНЫХ ВИРУСНОЙ ПАТОЛОГИЕЙ РОГОВИЦЫ

2015 ◽  
Vol 5 (02) ◽  
pp. 8
Author(s):  
V. A. Ulyanov ◽  
L. N. Velichko ◽  
A. V. Bogdanova ◽  
M. B. Makarova
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Markers ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Blood Lymphocytes ◽  
Tissue Therapy ◽  
Nanoparticles Of Silver

<p>The influence of the nanoparticles of silver on the expression of molecular markers activation of lymphoid cells CD7<sup>+</sup>, CD25<sup>+</sup>, CD38<sup>+</sup>, CD45<sup>+</sup>, CD54<sup>+</sup>, CD95<sup>+</sup>, CD150<sup>+</sup> and CD5<sup>+</sup> – marker of the autoimmune process, as well as on phagocytic activity of neutrophils in patients with viral pathologies of the cornea was studied <em>in vitro</em>. In the Laboratory of Immunology, SI Institute of Eye Diseases and Tissue Therapy NAMS of Ukraine was developed technique of cultivation of peripheral blood lymphocytes with immunomodulation drugs, followed by determination of changes in the level of expression of molecular markers of lymphocyte activation. Assessment of the level of expression of molecular markers of activation of peripheral blood lymphocytes was performed method using a panel of monoclonal antibodies, CD5<sup>+</sup>, CD7<sup>+</sup>, CD25<sup>+</sup>, CD38<sup>+</sup>, CD45<sup>+</sup>, CD54<sup>+</sup>, CD95<sup>+</sup> and CD 150<sup>+</sup>. The study was conducted <em>in vitro</em> with the peripheral lymphocytes the blood of 23 patients of viral pathology of the cornea. Our studies of the effects of nanosilver particles <em>in vitro</em> on the state of expression of molecular markers of activation of peripheral blood lymphocytes and phagocytic activity of neutrophils in patients with viral corneal pathology, showed a significant increase in the level of expression of the CD7<sup>+</sup>, CD25<sup>+</sup>, CD45<sup>+</sup> and phagocytic activity of neutrophils after application silver nanoparticles.</p> <p><em>Key words: immune system, nanoparticles of silver, monoclonal antibodies, molecular markers activation.</em></p>

scholarly journals Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes [see comments]

Blood ◽  
1992 ◽  
Vol 80 (11) ◽  
pp. 2735-2739 ◽  
Author(s):  
PM Chaudhary ◽  
EB Mechetner ◽  
IB Roninson
Keyword(s):  
Multidrug Resistance ◽  
Peripheral Blood ◽  
Fluorescent Dyes ◽  
Efflux Pump ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
P Glycoprotein

P-glycoprotein (P-gp), the product of the MDR1 (multidrug resistance) gene, is a transmembrane efflux pump for different lipophilic compounds, including many anticancer drugs and fluorescent dyes. We have previously reported that the efflux of fluorescent dyes from lymphoid cells of human bone marrow was directly correlated with the cellular P-gp content. In the present study, we show that human peripheral blood lymphocytes (PBL) also express P-gp, and that P-gp expression correlates with the efflux of fluorescent dyes from PBL. This efflux was suppressed not only by chemical inhibitors of P-gp but also by a P-gp-specific monoclonal antibody UIC2, thus providing direct evidence that it was mediated by P-gp. We have also characterized dye efflux and UIC2 reactivity in specific PBL subsets. P-gp was expressed in the majority of CD56+, CD8+, and CD20+ lymphocytes, but in less than one half of CD4+ cells. P-gp-mediated dye efflux was highly heterogeneous relative to the expression of CD56RA, CD56RO, Leu-8, and HLA-DR antigens. No significant P-gp activity was detectable in CD14+ monocytes. MDR1 expression in normal lymphocytes may be a determinant of multidrug resistance in the corresponding malignancies.

scholarly journals Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes [see comments]

Blood ◽  
1992 ◽  
Vol 80 (11) ◽  
pp. 2735-2739 ◽  
Author(s):  
PM Chaudhary ◽  
EB Mechetner ◽  
IB Roninson
Keyword(s):  
Multidrug Resistance ◽  
Peripheral Blood ◽  
Fluorescent Dyes ◽  
Efflux Pump ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
P Glycoprotein

Abstract P-glycoprotein (P-gp), the product of the MDR1 (multidrug resistance) gene, is a transmembrane efflux pump for different lipophilic compounds, including many anticancer drugs and fluorescent dyes. We have previously reported that the efflux of fluorescent dyes from lymphoid cells of human bone marrow was directly correlated with the cellular P-gp content. In the present study, we show that human peripheral blood lymphocytes (PBL) also express P-gp, and that P-gp expression correlates with the efflux of fluorescent dyes from PBL. This efflux was suppressed not only by chemical inhibitors of P-gp but also by a P-gp-specific monoclonal antibody UIC2, thus providing direct evidence that it was mediated by P-gp. We have also characterized dye efflux and UIC2 reactivity in specific PBL subsets. P-gp was expressed in the majority of CD56+, CD8+, and CD20+ lymphocytes, but in less than one half of CD4+ cells. P-gp-mediated dye efflux was highly heterogeneous relative to the expression of CD56RA, CD56RO, Leu-8, and HLA-DR antigens. No significant P-gp activity was detectable in CD14+ monocytes. MDR1 expression in normal lymphocytes may be a determinant of multidrug resistance in the corresponding malignancies.

scholarly journals Studies on correlations between immunophenotype and the indices of metabolic enzyme activity of blood lymphocytes in children with hypertrophy of the pharyngeal tonsils

2020 ◽  
Vol 22 (1) ◽  
pp. 165-170
Author(s):  
L. M. Kurtasova ◽  
N. A. Shakina ◽  
T. V. Lubnina
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Metabolic Enzyme ◽  
Control Group ◽  
Healthy Children ◽  
Blood Lymphocytes ◽  
Positive Correlations ◽  
Flow Cytofluorimetry ◽  
Activity Indices

The objective of our study was to evaluate correlation between the immune pheno-type and activity indices of NAD (P)-dependent dehydrogenases in peripheral blood lymphocytes in young children with hypertrophy of the pharyngeal tonsil (HPT). We have examined 57 children, 1-3 years of age, with hypertrophy of the pharyngeal tonsils (HPT). The control group consisted of 35 healthy children of the same age. The numbers of CD3+, CD4+, CD8+, CD19+, CD16+/56+ lymphoid cells in peripheral blood were determined by flow cytofluorimetry technique. Activity of NAD (P)-dependant dehydrogenases in peripheral blood lymphocytes was studied using bioluminescent method as described elsewhere (А. Savchenko, L. Suntsova, 1989). Correlation analysis has revealed an increase of positive correlations, a decrease of the correlation strength, and emergence of new connections between phenotype and activity indices of NAD (P)-dependent dehydrogenases in peripheral blood lymphocytes in children with hypertrophy of pharyngeal tonsils (HPT). Specific correlation patterns between the phenotype and activity indices of NAD (P)-dependent dehydrogenases in peripheral blood lymphocytes have been revealed in children with hypertrophy of pharyngeal tonsils (HPT).

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