scholarly journals Studies on correlations between immunophenotype and the indices of metabolic enzyme activity of blood lymphocytes in children with hypertrophy of the pharyngeal tonsils

2020 ◽  
Vol 22 (1) ◽  
pp. 165-170
Author(s):  
L. M. Kurtasova ◽  
N. A. Shakina ◽  
T. V. Lubnina
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Metabolic Enzyme ◽  
Control Group ◽  
Healthy Children ◽  
Blood Lymphocytes ◽  
Positive Correlations ◽  
Flow Cytofluorimetry ◽  
Activity Indices

The objective of our study was to evaluate correlation between the immune pheno-type and activity indices of NAD (P)-dependent dehydrogenases in peripheral blood lymphocytes in young children with hypertrophy of the pharyngeal tonsil (HPT). We have examined 57 children, 1-3 years of age, with hypertrophy of the pharyngeal tonsils (HPT). The control group consisted of 35 healthy children of the same age. The numbers of CD3+, CD4+, CD8+, CD19+, CD16+/56+ lymphoid cells in peripheral blood were determined by flow cytofluorimetry technique. Activity of NAD (P)-dependant dehydrogenases in peripheral blood lymphocytes was studied using bioluminescent method as described elsewhere (А. Savchenko, L. Suntsova, 1989). Correlation analysis has revealed an increase of positive correlations, a decrease of the correlation strength, and emergence of new connections between phenotype and activity indices of NAD (P)-dependent dehydrogenases in peripheral blood lymphocytes in children with hypertrophy of pharyngeal tonsils (HPT). Specific correlation patterns between the phenotype and activity indices of NAD (P)-dependent dehydrogenases in peripheral blood lymphocytes have been revealed in children with hypertrophy of pharyngeal tonsils (HPT).

scholarly journals Chromosome Aberrations in Peripheral Blood Lymphocytes of Croatian Hospital Staff Occupationally Exposed to Low Levels of Ionising Radiation

2008 ◽  
Vol 59 (4) ◽  
pp. 251-259 ◽  
Author(s):  
Vilena Kašuba ◽  
Ružica Rozgaj ◽  
Anamarija Jazbec
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Direct Detection ◽  
Hospital Staff ◽  
Peripheral Blood Lymphocytes ◽  
Ionising Radiation ◽  
Control Group ◽  
Blood Lymphocytes ◽  
Occupationally Exposed ◽  
Low Levels

Chromosome Aberrations in Peripheral Blood Lymphocytes of Croatian Hospital Staff Occupationally Exposed to Low Levels of Ionising RadiationMedical staff is an occupational group exposed to different agents suspected to induce genetic damage. Among them ionising radiation is the most studied. Cytogenetic analysis of human chromosomes in peripheral lymphocytes allows direct detection of mutation in somatic cells. This study investigated the cytogenetic effects of low-level ionising x-radiation in 48-hour peripheral blood lymphocyte cultures sampled from 765 hospital staff occupationally exposed to several agents known or suspected to induce chromosome damage and compared them with 200 control subjects. The exposed subjects were divided in eight (8) groups according to their specialities and job titles. The exposed groups manifested an increase in all types of chromosome aberrations. Acentric fragments were the most frequent chromosome-type aberration. Dicentric chromosomes were statistically significant only in urologists/gynaecologists. Age and smoking significantly influenced the incidence of dicentrics in the exposed groups. The frequency of ring chromosomes was low in all exposed groups (range: 0-2), and none were found in the control group. These findings indicate the importance of periodic medical checkups of hospital staff occupationally exposed to low doses of ionising radiation. The purpose is to create an individual cytogenetic register, where changes could evidence individual risks.

scholarly journals Sezary cell morphology induced in peripheral blood lymphocytes: re- evaluation

Blood ◽  
1989 ◽  
Vol 73 (6) ◽  
pp. 1603-1607
Author(s):  
AC Chu ◽  
JF Morris
Keyword(s):  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Lymphocytes ◽  
Epidermal Cells ◽  
Lymphoid Cells ◽  
T Helper ◽  
Normal Peripheral Blood ◽  
Blood Lymphocytes ◽  
Driven System ◽  
Sézary Cells

In this study we examined the effect of mitogens and epidermal cells in inducing a Sezary cell morphology in normal peripheral blood lymphocytes. Peripheral blood mononuclear cells from six healthy volunteers were stimulated with the mitogens phytohemaglutinin and concanavalin A, and also cocultivated with human epidermal cell cultures. Incubation times with mitogens and epidermal cells were four days and stimulation of the lymphocytes by mitogens was confirmed by standard 3H-thymidine uptake. Standard transmission electron microscopy showed that in the mitogen-driven system 20% to 60% (33 +/- 15%) and in the epidermal cell-driven system 5% to 15% (8 +/- 4%) of the lymphoid cells exhibited mild to moderate indentation of the nuclei with nuclear contour indices (NCI) of 4.6 to 6.5 but no Sezary cells were observed (cells with NCI greater than 6.5 and up to 19.2). In the mitogen- stimulated preparation 2% to 5% (3 +/- 1%) of the lymphoid cells showed nuclear multilobulation resembling the cells seen in adult T cell lymphoma/leukemia. Incubation of mononuclear cells for longer periods of up to 4 weeks with mitogens and exogenous IL-2 resulted in no further morphologic changes. Using an indirect immunogold technique at the electron microscopic level, the cells showing nuclear indentation or lobulation were shown to bear both T helper (CD4) and T suppressor (CD8) cell phenotypes in a similar ratio to the total numbers of T helper and T suppressor cells present. Mitogens and epidermal cells are thus not able to induce a morphologic change to Sezary cells in normal peripheral blood lymphocytes.

scholarly journals Sezary cell morphology induced in peripheral blood lymphocytes: re- evaluation

Blood ◽  
1989 ◽  
Vol 73 (6) ◽  
pp. 1603-1607 ◽  
Author(s):  
AC Chu ◽  
JF Morris
Keyword(s):  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Lymphocytes ◽  
Epidermal Cells ◽  
Lymphoid Cells ◽  
T Helper ◽  
Normal Peripheral Blood ◽  
Blood Lymphocytes ◽  
Driven System ◽  
Sézary Cells

Abstract In this study we examined the effect of mitogens and epidermal cells in inducing a Sezary cell morphology in normal peripheral blood lymphocytes. Peripheral blood mononuclear cells from six healthy volunteers were stimulated with the mitogens phytohemaglutinin and concanavalin A, and also cocultivated with human epidermal cell cultures. Incubation times with mitogens and epidermal cells were four days and stimulation of the lymphocytes by mitogens was confirmed by standard 3H-thymidine uptake. Standard transmission electron microscopy showed that in the mitogen-driven system 20% to 60% (33 +/- 15%) and in the epidermal cell-driven system 5% to 15% (8 +/- 4%) of the lymphoid cells exhibited mild to moderate indentation of the nuclei with nuclear contour indices (NCI) of 4.6 to 6.5 but no Sezary cells were observed (cells with NCI greater than 6.5 and up to 19.2). In the mitogen- stimulated preparation 2% to 5% (3 +/- 1%) of the lymphoid cells showed nuclear multilobulation resembling the cells seen in adult T cell lymphoma/leukemia. Incubation of mononuclear cells for longer periods of up to 4 weeks with mitogens and exogenous IL-2 resulted in no further morphologic changes. Using an indirect immunogold technique at the electron microscopic level, the cells showing nuclear indentation or lobulation were shown to bear both T helper (CD4) and T suppressor (CD8) cell phenotypes in a similar ratio to the total numbers of T helper and T suppressor cells present. Mitogens and epidermal cells are thus not able to induce a morphologic change to Sezary cells in normal peripheral blood lymphocytes.

Melatonin a Promising Candidate for DNA Double-Stranded Breaks Reduction in Patients Undergoing Abdomen-Pelvis Computed Tomography Examinations

2020 ◽  
Vol 20 (7) ◽  
pp. 859-864
Author(s):  
Ali Eskandari ◽  
Aziz Mahmoudzadeh ◽  
Alireza Shirazi ◽  
Farid Esmaely ◽  
Carla Carnovale ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Oral Dose ◽  
Single Oral Dose ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Promising Candidate ◽  
Blood Lymphocytes ◽  
Ct Examination

Background and Objective: Cancer incidence is 24% higher in children and young adults exposed to Computed Tomography (CT) scans than those unexposed. Non-repairing of ionizing radiation-induced DNA Double-Strand Breaks (DSBs) can initiate carcinogenesis. In the present study, we aimed to investigate the radioprotective potential of melatonin against DSBs in peripheral blood lymphocytes of patients undergoing abdomen-pelvis CT examinations. Methods: This double-blind, placebo-controlled clinical trial was conducted on thirty patients. These patients were divided into two groups; group one (control) patients who have undergone the CT examination received a single oral dose of placebo, while in group two, patients received a single oral dose of 100mg melatonin. In both the groups, blood samples were collected 5-10min before and 30 minutes after the CT examination. The lymphocytes from these samples were isolated and DSBs were analyzed using γH2AX immunofluorescence microscopy. Results: Compared to the control group, the use of melatonin 1h before the CT examination caused a significant reduction in γH2AX-foci, indicating a reduction in DSBs. In addition, no side effect was observed in patients following 100mg melatonin administration. Conclusion: For the first time, this study has shown that melatonin has protective effects against radiationinduced genotoxicity in peripheral blood lymphocytes of patients undergoing abdomen-pelvis CT examinations. Therefore, melatonin can be considered as a promising candidate for reducing DSBs in patients undergoing abdomen-pelvis CT examinations.

Genetic variation and risk of DNA damage in peripheral blood lymphocytes of Iranian formaldehyde-exposed workers

2017 ◽  
Vol 37 (7) ◽  
pp. 690-696
Author(s):  
Rezvan Zendehdel ◽  
Parviz Abdolmaleki ◽  
Fatemeh Javani Jouni ◽  
Mohammad Mazinani
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Control Population ◽  
Control Group ◽  
Special Role ◽  
Effective Parameters ◽  
Blood Lymphocytes ◽  
Exposed Population

Formaldehyde (FA) has given positive results for the genetic damage evaluated in human peripheral blood lymphocytes. Polymorphism plays a special role in the toxicity of chemicals. DNA damage in blood cells was evaluated in workers who were occupationally exposed to FA. This study tested the association of alcohol dehydrogenase III (ADH3) and manganese-superoxide dismutase (Mn-SOD) gene polymorphism, with DNA damage of peripheral blood lymphocytes. Gene polymorphisms were evaluated in 54 workers from melamine dinnerware workshops and 34 workers from dairy production workshops. The control and exposed population were matched based on age, smoking, work history and socioeconomic status. DNA damage was evaluated by alkaline comet assay in the peripheral blood lymphocytes. ADH3 and Mn-SOD genotypes were determined in all workers using polymerase chain reaction–restriction fragment length polymorphism method. DNA damage of lymphocyte cells was significantly higher ( p < 0.001) in the exposed individuals in comparison with the control population. The examinations of the exposed group had revealed significantly higher ( p < 0.01) variant of Val/Val of Mn-SOD compared to control group. In workers with ADH32–2 variant, significantly higher levels of DNA damage ( p < 0.01) were observed than the control population with the same genotype. It could be concluded that enzymes involved in the metabolism of FA and oxidative stress controlling are two important effective parameters for DNA damage of peripheral blood lymphocytes in exposed people. These results are regarded as a pioneer investigation for the management of health risks assessment.

scholarly journals Effects of sevoflurane exposure on apoptosis and cell cycle of peripheral blood lymphocytes, and immunologic function

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhimin Ji ◽  
Wanjun Wu ◽  
Fan Zhou ◽  
Junfang Hu ◽  
Qiuping Xu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Operating Room ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Venous Blood ◽  
Peripheral Blood Lymphocytes ◽  
Exposed Group ◽  
Control Group ◽  
Blood Lymphocytes

Abstract Background Waste anesthetic gases (WAGs) leaked from new-type halogenated inhalational anesthetics such as sevoflurane have been were reported to pose a risk for the health of operating room personnel. The effects of WAGs on peripheral blood lymphocytes, however, remain yet controversial. The present study was undertaken to examine the effects of occupational sevoflurane exposure on the peripheral blood lymphocytes of medical personnel who work in the operating room. Methods A cohort of 56 medical residents were divided into exposed group (n = 28) and control group (non-exposed group) (n = 28). Gas chromatography was used to measure the concentration of sevoflurane in the medical resident’s breathing zone during surgeries under inhalation anesthesia in the exposure group. The gas collection lasted an hour. Peripheral blood lymphocytes were isolated from venous blood, and then apoptosis and cell cycle were analyzed by flow cytometry. EDTA-anticoagulated whole blood was harvested to analyze the lymphocyte subsets by flow cytometry. Immunoglobulins (IgA, IgM, IgG) were quantified by immunoturbidimetry. Results The average concentration of sevoflurane in the exposed group was 1.03 ppm with a range from 0.03 ppm to 2.24 ppm. No significant effects were found on the apoptosis rates or cell cycles of peripheral blood lymphocytes in the exposed group relative to the control group (P > 0.05). Similarly, there were no significant differences in the lymphocyte subsets or the levels of immunoglobulins (IgA, IgM, IgG) between the two groups (P > 0.05). Conclusions Occupational exposure to low-level sevoflurane has no significant effect on the peripheral blood lymphocytes of operating room staff, but this conclusion needs to be confirmed by multicenter and long-term follow-up studies with large samples. Trial registration number and date of registration ChiCTR2000040772, December 9, 2020 (Retrospective registration).

scholarly journals Effect of Sirolimus on the Level of Peripheral Blood Lymphocyte Autophagy in Children With Systemic Lupus Erythematosus

2021 ◽  
Vol 9 ◽  
Author(s):  
Xinliang Wang ◽  
Qingxiao Su ◽  
Zhiyan Dou ◽  
Xue Zhao ◽  
Naiqi Zhang ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Treatment Group ◽  
Lupus Erythematosus ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Complement C3 ◽  
Healthy Children ◽  
Systemic Lupus ◽  
Traditional Treatment ◽  
Blood Lymphocytes

Background: To observe the changes of autophagy-related protein levels in peripheral blood lymphocytes before and after sirolimus treatment in children with systemic lupus erythematosus (SLE).Methods: Children with SLE were randomly divided into two groups, 28 in the traditional treatment group and 28 in the sirolimus group. Fifteen healthy children who were in the same period were collected as the normal control group. Clinical laboratory indexes, the percentage of routine lymphocytes, complement C3, complement C4, serum Anti-dsDNA and SLEDAI were detected.Results: At 3 and 6 months after treatment, compared with the traditional treatment group, the percentage of routine lymphocytes in the sirolimus group increased (P = 0.03), SLEDAI score and positive rate of Anti-dsDNA decreased (P = 0.01). Compared with normal children, the expression of microtubule-associated protein 1 light chain 3 (LC3) protein in peripheral blood lymphocytes was significantly higher (P = 0.006); peripheral blood expression of P62/SQSTM1 (sequestosome 1) protein in lymphocytes decreased (P = 0.02).Conclusion: Sirolimus can play a role in the treatment of systemic lupus erythematosus by regulating the level of autophagy.

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