Determination of unbound fraction of disopyramide in plasma: A comparison of equilibrium dialysis, ultrafiltration through dialysis membranes and ultrafree anticonvulsant drug filters

1982 ◽  
Vol 7 (1) ◽  
pp. 7-14 ◽  
Author(s):  
R.L.G. Norris ◽  
J.T. Ahokas ◽  
P.J. Ravenscroft
Keyword(s):  
Equilibrium Dialysis ◽  
Anticonvulsant Drug ◽  
Unbound Fraction ◽  
Dialysis Membranes

scholarly journals Comparison of Microscale Ultracentrifugation and Equilibrium Dialysis for the Determination of the Unbound Fraction of Theophylline in Human Serum

1989 ◽  
Vol 26 (2) ◽  
pp. 185-188 ◽  
Author(s):  
K McKenzie ◽  
I D Watson ◽  
M J Stewart
Keyword(s):  
Human Serum ◽  
Equilibrium Dialysis ◽  
Cost Effective ◽  
Free Drug ◽  
Effective Technique ◽  
Unbound Fraction ◽  
Cost Effective Technique ◽  
Dialysis Method

A microscale ultracentrifugation procedure for the measurement of free theophylline in serum is described and evaluated by comparison with an equilibrium dialysis method. Provided an ultracentrifuge is available, micro-ultracentrifugation is a simple, cost-effective technique for the routine determination of free drug fractions.

scholarly journals Determination of dolutegravir's unbound fraction in human plasma using validated equilibrium dialysis and LC-MS/MS methods

2018 ◽  
Vol 479 ◽  
pp. 56-65 ◽  
Author(s):  
David Metsu ◽  
Thomas Lanot ◽  
François Fraissinet ◽  
Mélanie Picot ◽  
Didier Concordet ◽  
...  
Keyword(s):  
Human Plasma ◽  
Equilibrium Dialysis ◽  
Unbound Fraction

Tears as the Best Practical Indicator of the Unbound Fraction of an Anticonvulsant Drug

Epilepsia ◽  
1979 ◽  
Vol 20 (6) ◽  
pp. 705-710 ◽  
Author(s):  
Francesco Monaco ◽  
Roberto Mutani ◽  
Camillo Mastropaolo ◽  
Massimo Tondi
Keyword(s):  
Anticonvulsant Drug ◽  
Unbound Fraction

A Review on Analytical Method for Determination of Lamotrigine in Bulk and Pharmaceutical Dosage Form

2021 ◽  
pp. 229-234
Author(s):  
Rutuja S Nalkar ◽  
Suhas S Siddheshwar ◽  
Mahesh H Kolhe
Keyword(s):  
Side Effects ◽  
Blood Cells ◽  
Anticonvulsant Drug ◽  
Mood Stabilizer ◽  
Stevens Johnson Syndrome ◽  
Partial Seizures ◽  
Pharmaceutical Dosage Form ◽  
Johnson Syndrome ◽  
Clonic Seizures

Lamotrigine is an anticonvulsant drug used in the treatment of epilepsy & bipolar disorder/major affective disorder (manic depression). Lamotrigine is and antiepileptic drug of phenyltriazine class. For epilepsy it is used to treat the partial seizures, primary and secondary tonic-clonic seizures, and seizures associated with the Lennox-Gastuat syndrome and are chemically unrelated to the other anticonvulsants. Lamotrigine is a phenyltriazine that has comparatively few side-effects and it does not requires blood monitoring/observance in monotherapy. It additionally acts as a mood stabilizer. Common side-effects of lamotrigine include, nausea, sleepiness, headache, vomiting, trouble/bother with co-ordination and rash. Serious side-effects include in, lack of red blood cells, accumulated in risk of suicide, Stevens-Johnson syndrome and allergy. It issues that use of lamotrigine throughout pregnancy or breastfeeding it’s going to lead/result in harm/damage.

Development and Validation of HPLC Determination of related Substances in A Novel Anticonvulsant agent Epimidin

2021 ◽  
pp. 3223-3231
Author(s):  
Hanna I. Severina ◽  
Svitlana M. Gubar ◽  
Ivan V. Bezruk ◽  
Anna S. Materiienko ◽  
Liudas Ivanauskas ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Phosphate Buffer Solution ◽  
Anticonvulsant Drug ◽  
Strong Acid ◽  
Gradient Elution ◽  
Stress Conditions ◽  
Drug Candidate ◽  
Buffer Solution ◽  
Related Substances

1-(4-methoxyphenyl)-5-[2-[4-(4-methoxyphenyl)piperazin-1-yl]-2-oxo-ethyl]pyrazolo[3,4-d]pyrimidin-4-one has been reported as a promising new anticonvulsant drug candidate with a code name “Epimidin”. A new HPLC method for the related substances determination of potential active pharmaceutical ingredient has been developed and validated. The method uses ACE C18 column (250x4.6mm, 5µm) and gradient elution. Mobile phase consisted of a mixture of methanol R (mobile phase A) and phosphate buffer solution with triethanolamine, adjusted to pH 7.0 (mobile phase B). During the analysis, the ratio of mobile phases was changing according to a gradient mode at a flow rate of 1ml/min. The DAD detection was set at 240nm. The method was validated according to the ICH guidelines and requirements of State Pharmacopoeia of Ukraine. Drug substance was thoroughly explored for stability assessments under various stress conditions such as high temperature, as well as the influence of strong acid and base and oxidizing agents. The obtained solutions were analyzed by HPLC and LC/MS. It has been shown that the substance Epimidin was not resistant to the action of peroxide, alkali and acid decomposition – the mentioned stress conditions lead to the formation of unidentified impurities.

scholarly journals Interaction of rice (Oryza sativa) lectin with N-acetylglucosaminides. Fluorescence studies

1985 ◽  
Vol 229 (3) ◽  
pp. 687-692 ◽  
Author(s):  
F Tabary ◽  
J P Frénoy
Keyword(s):  
Oryza Sativa ◽  
Binding Sites ◽  
Wheat Germ ◽  
Equilibrium Dialysis ◽  
Blue Shift ◽  
Association Constants ◽  
Fluorescence Studies ◽  
Saccharide Binding ◽  
Binding Enthalpy

The interaction of lectin isolated from rice (Oryza sativa) embryos with N-acetylglucosaminides was studied by equilibrium dialysis and fluorescence. Equilibrium dialysis with 4-methylumbelliferyl-(GlcNac)2 showed that rice lectin (Mr 38000) contains four equivalent saccharide-binding sites. Addition of the N-acetylglucosaminides GlcNac, (GlcNac)2 and (GlcNac)3 enhanced the intrinsic fluorescence of rice lectin and this was accompanied by a 10nm blue-shift of its maximum fluorescence with (GlcNac)2 and (GlcNac)3. These changes in intensity allowed determination of the association constants, which increased with the number of saccharide units: at 20 degrees C, Ka = (1.3 +/- 0.1) X 10(3), (5.1 +/- 0.4) X 10(4) and (2.6 +/- 0.1) X 10(5) M−1 for GlcNac, (GlcNac)2 and (GlcNac)3 respectively. The binding enthalpy, delta H0, for the three glucosaminides were very low and ranged from −12.1 to −20.6 kJ X mol-1. The results are compared with those obtained with wheat-germ agglutinin, another GlcNac-specific gramineaous lectin.

scholarly journals Electrochemical characterization and determination of carbamazepine as pharmaceutical standard and tablet content on gold electrode

2014 ◽  
Vol 68 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Nemanja Trisovic ◽  
Bojan Bozic ◽  
Slobodan Petrovic ◽  
Svetlana Tadic ◽  
Milka Avramov-Ivic
Keyword(s):  
Cyclic Voltammetry ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Gold Electrode ◽  
Anticonvulsant Drug ◽  
Voltammetric Techniques ◽  
Anodic Behaviour

The anodic behaviour of carbamazepine (CBZ), an anticonvulsant drug, has been studied on gold electrode in 0.1 mol dm-3 phosphate buffer of pH 7.0 by using cyclic voltammetry. It has been found that the value of the oxidative current of pure CBZ at +0.90 V is a linear function of the concentration in a range from 1.0?10-7 to 1.0?10?4 mol dm?3. The detection of CBZ in the concentration of 1.0?10-8 mol dm-3 is among the lowest that have been reported for this drug using voltammetric techniques. CBZ as a content of tablet Galepsine? has been quantitatively determined. It has also been demonstrated that the modification of gold electrode with bovine serum albumin (BSA) results in a decrease of the oxidative peak current due to the binding of the drug to BSA.

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