Purification of bovine conglutinin using pepsin digestion

1981 ◽  
Vol 18 (2) ◽  
pp. 85-89 ◽  
Author(s):  
M.A. Maire ◽  
M. Barnet ◽  
P.H. Lambert
Keyword(s):  
Pepsin Digestion

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

Collagen characterization in volume-overload- and pressure-overload-induced cardiac hypertrophy in minipigs

1993 ◽  
Vol 265 (2) ◽  
pp. H434-H438 ◽  
Author(s):  
J. Harper ◽  
E. Harper ◽  
J. W. Covell
Keyword(s):  
Cardiac Hypertrophy ◽  
Porcine Model ◽  
Pressure Overload ◽  
Volume Overload ◽  
Heart Tissue ◽  
Canine Heart ◽  
Cross Link ◽  
Collagen Types ◽  
Collagen Concentration ◽  
Pepsin Digestion

Previous studies in several different species have shown reduced extractability of collagens in some types of cardiac hypertrophy (volume overload) but not others (pressure overload). The objective of the present study was to examine collagen proteins from the same species (minipigs) with both pressure-overload- and volume-overload-induced cardiac hypertrophy. Hypertrophy was induced by two methods: thoracic banding of the aorta to create pressure overload and arteriovenous shunt to cause volume overload in a porcine model. Collagen types I, III, IV, and V were isolated by pepsin digestion from normal and hypertrophied pig left ventricle tissues. Types I and III collagens from normal and hypertrophied samples, when separated from types IV and V, were digested with cyanogen bromide (CB), and the peptides were examined. Collagen concentration was increased in myocardium removed from hearts subjected to volume overload and unchanged in hearts subjected to pressure overload. The extractability of total collagen was unaffected in pressure-overloaded left ventricles but lower in samples from volume-overloaded hearts. CB digestion cleaved all of the types I and III collagens into similar smaller CB peptides with the exception of a 100-kDa peptide that was observed in both control and hypertrophied hearts. This peptide corresponds to one of the high-molecular-weight peptides found in canine heart tissue. The mature collagen cross-link hydroxylysylpyridinoline (HP) was identified in normal and hypertrophied types I and III collagen from porcine sources. Pressure-overload- and volume-overload-induced cardiac hypertrophy in the pig produced different alterations in the extracellular matrix.(ABSTRACT TRUNCATED AT 250 WORDS)

Ultrastructure, organization and cytochemistry of cytoplasmic crystalline inclusions in Origanum dictamnus L. leaf chlorenchyma cells

1983 ◽  
Vol 64 (1) ◽  
pp. 231-244
Author(s):  
A.M. Bosabalidis ◽  
D. Papadopoulos
Keyword(s):  
Rectangular Prism ◽  
Square Lattice ◽  
Tetragonal Symmetry ◽  
Pepsin Digestion ◽  
Crystalline Inclusions ◽  
Double Helices ◽  
Diffraction Patterns ◽  
Crystallographic Planes

In the cytoplasm of chlorenchyma cells in primary Origanum dictamnus leaves, rectangular prism-like crystals occur, which, as shown by pepsin digestion, are proteinaceous. In sections they usually show either a square lattice or striations running parallel to the short or long axis. In both cases the interspacings are estimated to be about 100 A, suggesting that the arrangement of the crystallographic planes possesses tetragonal symmetry. High magnifications of the crystalline inclusions together with analysis of their diffraction patterns showed that the striations are composed of double helices of protein macromolecules. In leaves 3–4 mm long, eroded crystals are often observed. When leaves are larger than 5 mm, no crystalline bodies can be identified in chlorenchyma cells. It is possible that they represent a storage form of protein, which is used later by the developing cells.

Evidence For Four Classes of Microtubules in Individual Cells

1967 ◽  
Vol 2 (2) ◽  
pp. 169-192
Author(s):  
O. BEHNKE ◽  
A. FORER
Keyword(s):  
Colchicine Treatment ◽  
Negative Staining ◽  
The Other ◽  
Pepsin Digestion ◽  
Sperm Tail ◽  
Staining Techniques ◽  
Cytoplasmic Microtubules ◽  
Experimental Treatments

Experiments were performed on crane-fly spermatids (Nephrotoma suturalis Loew), ratsperm, and rat tracheal cilia to test whether all microtubules respond in the same way to different treatments. Crane-fly spermatids contain cytoplasmic microtubules, accessory tubules, and the 9 + 2 complex of tubules; rat sperm and rat tracheal cilia contain only the 9 + 2 tubules. Crane-fly spermatid tubules responded to the experimental treatments as follows. After colchicine treatment, or storage at 0 °C, the cytoplasmic microtubules disappeared, while the 9 + 2 tubules were normal. After storage at 50 °C the cytoplasmic microtubules disappeared, and then the 9 + 2 tubules were affected: first the central tubules and B-tubules were affected, and later the A-tubules. After brief pepsin treatment, the 9 doublet tubules disappeared, while the other tubules appeared normal; after prolonged pepsin treatment the accessory, central, and cytoplasmic tubules disappeared. After negative staining at pH 7, the cytoplasmic microtubules were never seen, the central tubules were only sometimes seen, the B-tubules were sometimes fragmented, and the A-tubules were intact. On the basis of these responses, it was concluded that there are 4 classes of tubules in crane-fly spermatids, namely cytoplasmic microtubules; accessory tubules and central tubules (of the 9 + 2 complex); B-tubules (of the 9 + 2 complex); and A-tubules (of the 9 + 2 complex). At least some of the different responses appeared to be due to intrinsic physical and/or chemical differences between the tubules themselves. Pepsin digestion and negative staining of rat sperm tails gave results similar to those with crane-fly spermatids. In addition, the 9 + 2 tubules responded differently to pepsin digestion at different points along their length. This gradient of sensitivity was attributed to synthesis of new tubules occurring at one end of the sperm tail. Pepsin digestion and negative staining of rat tracheal cilia gave results similar to those with crane-fly spermatids and rat sperm tails. All the tubules had a similar substructure, as revealed by negative-staining techniques. It was concluded that microtubules are proteinaceous, at least in part, and that microtubules are different in composition from membranes. It is suggested that the walls of the B-tubules are composed of two materials--(1) the portions adjacent to the A-tubules, and (2) the remaining portion.

Chromopeptides from Phytochrome and Phycocyanin. NM R Studies o f the Pfr and Pr Chromophore of Phytochrome and E,Z Isomeric Chromophores of Phycocyanin

1983 ◽  
Vol 38 (5-6) ◽  
pp. 359-368 ◽  
Author(s):  
Fritz Thümmler ◽  
Wolfhart Rüdiger ◽  
Edmund Cmiel ◽  
Siegfried Schneider
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Avena Sativa ◽  
Maximum Amount ◽  
Acid Interaction ◽  
Spirulina Maxima ◽  
Nmr Spectrum ◽  
Pepsin Digestion ◽  
H Nmr ◽  
Amino Acid Interaction

Chromopeptides were prepared by pepsin digestion of C-phycocyanin isolated from the cyano­bacterium Spirulina maxima and of phytochrome isolated from seedlings of Avena sativa L. The chromopeptides were characterized by amino acid analysis. The ZZZ configurated chromophore of the phycocyanin peptide was transformed into its ZZE configurated isomer by the method of Falk et al. (Mh. Chemie 111, 159- 175, 1980) which had previously been applied to biliverdins. The 500 MHz 1HNMR spectrum of the ZZE configurated chromopeptides confirmed that its chromophore has the 15 E configuration. Irradiation yielded the ZZZ configurated isomer for which the 1H NMR spectrum was also recorded. Native phytochrome was irradiated at 660 nm to yield the maximum amount of the Pfr from (about 75% of total phytochrome). By digestion in the dark the previously described Pfr chromopeptide was obtained. The 500 MHz 1H NMR spectrum was compared with that of the ZZE phycocyanin peptide. It confirmed the 15 E con­figuration of the Pfr chromopeptide. Irradiation yielded the 15 Z configurated Pr chromopeptide. Comparison of the high resolution 1HNMR spectra of Pfr and Pr chromopeptides revealed that not only the chromophore resonances but also those of some amino acids are changed by the Pfr → Pr chromopeptide phototransformation. The results are discussed in terms of chromophore amino acid interaction.

Equine F(ab')2-based antivenom preparation by simultaneous caprylic acid fractionation and pepsin digestion

Toxicon ◽  
2019 ◽  
Vol 159 ◽  
pp. S11
Author(s):  
Tihana Kurtović ◽  
Maja Lang Balija ◽  
Monika Tunjić ◽  
Marija Brgles ◽  
Dora Sviben ◽  
...  
Keyword(s):  
Caprylic Acid ◽  
Pepsin Digestion
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