Specificity of naturally occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus: Determination by an enzyme linked immunosorbent assay

1986 ◽  
Vol 12 (5-6) ◽  
pp. 251-256 ◽  
Author(s):  
Mitsuko Tauchi ◽  
Y. Kanai ◽  
H. Hashimoto ◽  
S. Hirose
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Immunosorbent Assay ◽  
Lupus Erythematosus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Systemic Lupus ◽  
Naturally Occurring ◽  
Naturally Occurring Antibodies

Enzyme-linked immunosorbent assay for detection of antibodies to extractable nuclear antigens in systemic lupus erythematosus, with nylon as solid phase.

1983 ◽  
Vol 29 (5) ◽  
pp. 823-827 ◽  
Author(s):  
M Ishaq ◽  
R Ali
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Immunosorbent Assay ◽  
Lupus Erythematosus ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Prolonged Storage ◽  
Antibody Activity ◽  
Systemic Lupus ◽  
Nuclear Antigens ◽  
As Solid Phase

Abstract In this enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against extractable nuclear antigens (ENA) in sera of patients with systemic lupus erythematosus (SLE), nylon is used as solid phase for antigen binding instead of the commonly used polystyrene surface. Optimal conditions for activation of the nylon beads, antigen coating, and other relevant factors have been investigated. We compared the incidence of anti-ENA antibodies in SLE, using chromogenic and fluorogenic enzyme substrates. Of SLE patients, 54% were positive for anti-ENA antibodies when chromogenic substrate was used as compared with 68% for fluorogenic substrate. Antibody activity against Sm and RNP antigens was distinguished on the basis of ribonuclease sensitivity of the RNP antigen. The method described offers advantages such as decreased background activity, increased surface area, facility for prolonged storage of antigen-coated solid phase, and miniaturization of the assay.

Procalcitonin and C-reactive protein as markers of infection in systemic lupus erythematosus: the controversy continues

Lupus ◽  
2018 ◽  
Vol 28 (11) ◽  
pp. 1329-1336 ◽  
Author(s):  
E El-serougy ◽  
H S Zayed ◽  
N M Ibrahim ◽  
L A Maged
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Bacterial Infection ◽  
Immunosorbent Assay ◽  
Lupus Erythematosus ◽  
High Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
C Reactive Protein ◽  
Systemic Lupus ◽  
Reactive Protein ◽  
Symptoms And Signs

Objective The objective of this paper is to investigate the utility of serum procalcitonin (PCT) and C-reactive protein (CRP) as markers of infection in systemic lupus erythematosus (SLE) patients. Patients and methods Sixty-nine SLE patients with symptoms and signs of infection proved by culture and/or a favorable response to antibiotics and 69 SLE patients without infection were included. Serum PCT and plasma high-sensitivity CRP were assessed by an enzyme-linked immunosorbent assay. Results SLE patients with infection had a significantly higher level of CRP than those without infection ((median (IQR) 104.5 (25.5–100.9) and 10.3 (5.4–23.1) mg/l, respectively), p<0.001). Conclusion Serum PCT could not differentiate SLE patients with or without bacterial infection in this study, while the utility of CRP as a marker of infection has been confirmed.

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