Fluorescent in situ hybridization (FISH) as a tool to identify chromosomal aberrations in human tumor cells

1993 ◽  
Vol 66 (2) ◽  
pp. 157
Author(s):  
Rachel A. Jesudasan ◽  
M. Rezaur Rahman ◽  
K.L. Ying ◽  
C. Patrick Reynolds ◽  
Eri S. Srivatsan
Keyword(s):  
In Situ Hybridization ◽  
Tumor Cells ◽  
Chromosomal Aberrations ◽  
Fluorescent In Situ Hybridization ◽  
Human Tumor ◽  
Human Tumor Cells

scholarly journals De Novo Balanced Translocation t (7;16) (p22.1; p11.2) Associated with Autistic Disorder

2008 ◽  
Vol 2008 ◽  
pp. 1-5 ◽  
Author(s):  
Nadia Bayou ◽  
Ridha M'rad ◽  
Ahlem Belhaj ◽  
Hussein Daoud ◽  
Lamia Ben Jemaa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Causal Relationship ◽  
Chromosomal Aberrations ◽  
Autistic Disorder ◽  
Fluorescent In Situ Hybridization ◽  
De Novo ◽  
Balanced Translocation ◽  
Bac Clones ◽  
High Incidence

The high incidence of de novo chromosomal aberrations in a population of persons with autism suggests a causal relationship between certain chromosomal aberrations and the occurrence of isolated idiopathic autism. We report on the clinical and cytogenetic findings in a male patient with autism, no physical abnormalities and a de novo balanced (7;16)(p22.1;p16.2) translocation. G-banded chromosomes and fluorescent in situ hybridization (FISH) were used to examine the patient's karyotype as well as his parents'. FISH with specific RP11-BAC clones mapping near 7p22.1 and 16p11.2 was used to refine the location of the breakpoints. This is, in the best of our knowledge, the first report of an individual with autism and this specific chromosomal aberration.

scholarly journals The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1465 ◽  
Author(s):  
Arutha Kulasinghe ◽  
Yenkai Lim ◽  
Joanna Kapeleris ◽  
Majid Warkiani ◽  
Ken O’Byrne ◽  
...  
Keyword(s):  
Lung Cancer ◽  
In Situ Hybridization ◽  
Tumor Cells ◽  
Anaplastic Lymphoma Kinase ◽  
Fluorescent In Situ Hybridization ◽  
Small Cell ◽  
Small Cell Lung ◽  
Anaplastic Lymphoma ◽  
Nsclc Patients

Tumor tissue biopsy is often limited for non-small cell lung cancer (NSCLC) patients and alternative sources of tumoral information are desirable to determine molecular alterations such as anaplastic lymphoma kinase (ALK) rearrangements. Circulating tumor cells (CTCs) are an appealing component of liquid biopsies, which can be sampled serially over the course of treatment. In this study, we enrolled a cohort of ALK-positive (n = 8) and ALK-negative (n = 12) NSCLC patients, enriched for CTCs using spiral microfluidic technology and performed DNA fluorescent in situ hybridization (FISH) for ALK. CTCs were identified in 12/20 NSCLC patients ranging from 1 to 26 CTCs/7.5 mL blood. Our study revealed that 3D imaging of CTCs for ALK translocations captured a well-defined separation of 3′ and 5′ signals indicative of ALK translocations and overlapping 3′/5′ signal was easily resolved by imaging through the nuclear volume. This study provides proof-of-principle for the use of 3D DNA FISH in the determination of CTC ALK translocations in NSCLC.

In situ DNA methylation analysis of human tumor cells, mapping of methylcytosine rich bands

1994 ◽  
Vol 77 (2) ◽  
pp. 150
Author(s):  
Agnès Barbin ◽  
Alain Niveleau ◽  
Bernard Dutrillaux ◽  
Bernard Malfoy ◽  
Claire A. Bourgeois
Keyword(s):  
Dna Methylation ◽  
Tumor Cells ◽  
Human Tumor ◽  
Methylation Analysis ◽  
Human Tumor Cells ◽  
Dna Methylation Analysis

1796: Analysis of Chromosomal Aneuploidy in Patients with Oligoasthenoteratozoospermia(OAT) Using Fluorescent in-situ Hybridization

2007 ◽  
Vol 177 (4S) ◽  
pp. 596-597
Author(s):  
Joseph P. Alukal ◽  
Bobby B. Najari ◽  
Wilson Chuang ◽  
Lata Murthy ◽  
Monica Lopez-Perdomo ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chromosomal Aneuploidy
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