Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells

2017 ◽  
pp. 133-141
Author(s):  
Marianne Oulhen ◽  
Emma Pailler ◽  
Vincent Faugeroux ◽  
Françoise Farace
Keyword(s):  
In Situ Hybridization ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Fluorescent In Situ Hybridization

Improvement of specific detection of circulating tumor cells using combined CD45 staining and fluorescence in situ hybridization

2014 ◽  
Vol 433 ◽  
pp. 69-75 ◽  
Author(s):  
Ning Ning ◽  
Ting Zhan ◽  
Yujuan Zhang ◽  
Qian Chen ◽  
Fengzhi Feng ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Specific Detection

Microsieve lab-chip device for rapid enumeration and fluorescence in situ hybridization of circulating tumor cells

Lab on a Chip ◽  
2012 ◽  
Vol 12 (21) ◽  
pp. 4388 ◽  
Author(s):  
Li Shi Lim ◽  
Min Hu ◽  
Mo Chao Huang ◽  
Wai Chye Cheong ◽  
Alfred Tau Liang Gan ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Rapid Enumeration

scholarly journals The Use of Three-Dimensional DNA Fluorescent In Situ Hybridization (3D DNA FISH) for the Detection of Anaplastic Lymphoma Kinase (ALK) in Non-Small Cell Lung Cancer (NSCLC) Circulating Tumor Cells

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1465 ◽  
Author(s):  
Arutha Kulasinghe ◽  
Yenkai Lim ◽  
Joanna Kapeleris ◽  
Majid Warkiani ◽  
Ken O’Byrne ◽  
...  
Keyword(s):  
Lung Cancer ◽  
In Situ Hybridization ◽  
Tumor Cells ◽  
Anaplastic Lymphoma Kinase ◽  
Fluorescent In Situ Hybridization ◽  
Small Cell ◽  
Small Cell Lung ◽  
Anaplastic Lymphoma ◽  
Nsclc Patients

Tumor tissue biopsy is often limited for non-small cell lung cancer (NSCLC) patients and alternative sources of tumoral information are desirable to determine molecular alterations such as anaplastic lymphoma kinase (ALK) rearrangements. Circulating tumor cells (CTCs) are an appealing component of liquid biopsies, which can be sampled serially over the course of treatment. In this study, we enrolled a cohort of ALK-positive (n = 8) and ALK-negative (n = 12) NSCLC patients, enriched for CTCs using spiral microfluidic technology and performed DNA fluorescent in situ hybridization (FISH) for ALK. CTCs were identified in 12/20 NSCLC patients ranging from 1 to 26 CTCs/7.5 mL blood. Our study revealed that 3D imaging of CTCs for ALK translocations captured a well-defined separation of 3′ and 5′ signals indicative of ALK translocations and overlapping 3′/5′ signal was easily resolved by imaging through the nuclear volume. This study provides proof-of-principle for the use of 3D DNA FISH in the determination of CTC ALK translocations in NSCLC.

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