Enzyme-linked immunosorbent assay for IgG and IgM antibodies against human parvovirus B19: use of monoclonal antibodies and viral antigen propagated in vitro

1989 ◽  
Vol 26 (2) ◽  
pp. 171-181 ◽  
Author(s):  
Yaegashi Nobuo ◽  
Shiraishi Hiroyuki ◽  
Tada Kohtaro ◽  
Yajima Akira ◽  
Sugamura Kazuo
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Viral Antigen ◽  
Parvovirus B19 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Parvovirus B19 ◽  
Igm Antibodies

scholarly journals Human parvovirus B19-induced anaemia in pre-school children in Ilorin, Nigeria

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Oluwaseyi S. Ashaka ◽  
Olajide O. Agbede ◽  
Adesuyi A. Omoare ◽  
Samuel K. Ernest
Keyword(s):  
Differential Diagnosis ◽  
Immunosorbent Assay ◽  
School Children ◽  
Parvovirus B19 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Parvovirus B19 ◽  
Igm Antibodies ◽  
Parvovirus B19 Infection ◽  
Assay Technique

Sera collected from 57 anaemic and 115 non-anaemic age-matched pre-school children in Ilorin, Nigeria, between November 2014 and December 2015 were assayed for human parvovirus B19-specific IgM antibodies by using the enzyme linked immunosorbent assay technique. A total of 17 (29.8%) anaemic children and 18 (15.7%) non-anaemic children were positive for parvovirus B19 infection. Infection with parvovirus B19 is common in this population, and screening for the virus during differential diagnosis is recommended.

scholarly journals Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Results

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

scholarly journals Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay.

1986 ◽  
Vol 24 (4) ◽  
pp. 522-526 ◽  
Author(s):  
L J Anderson ◽  
C Tsou ◽  
R A Parker ◽  
T L Chorba ◽  
H Wulff ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Parvovirus B19 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Parvovirus B19

scholarly journals Simple Histidine-Rich Protein 2 Double-Site Sandwich Enzyme-Linked Immunosorbent Assay for Use in Malaria Drug Sensitivity Testing

2005 ◽  
Vol 49 (8) ◽  
pp. 3575-3577 ◽  
Author(s):  
Harald Noedl ◽  
Jan Bronnert ◽  
Kritsanai Yingyuen ◽  
Bernhard Attlmayr ◽  
Herwig Kollaritsch ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Drug Sensitivity ◽  
Inhibitory Concentration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sensitivity Testing ◽  
Drug Sensitivity Test ◽  
Sensitivity Tests

ABSTRACT A simple double-site sandwich enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum in vitro drug sensitivity tests based on measuring histidine-rich protein 2 (HRP2) is presented. The ELISA uses two commercial monoclonal antibodies and provides a drastically cheaper alternative to the test kits previously used in the HRP2 drug sensitivity test. The assay is simple to establish and perform. The sensitivity is comparable and the drug sensitivity results very closely match those obtained with the commercial ELISA kits (R 2 = 0.979; P < 0.001; mean log difference at the 50% inhibitory concentration = 0.07).

scholarly journals PREVALENCE OF HUMAN PARVOVIRUS B19 IgG AND IgM ANTIBODIES AMONG PREGNANT WOMEN ATTENDING ANTENATAL CLINIC AT FEDERAL TEACHING HOSPITAL IDO-EKITI, NIGERIA.

2021 ◽  
Vol 15 (2) ◽  
pp. 10-15
Author(s):  
Richard Yomi Akele ◽  
Jennifer Tamuno Abelekum ◽  
Bernard Oluwapelum Oluboyo ◽  
Janet Funmilayo Akinseye ◽  
Seyi Samson Enitan ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Pregnant Women ◽  
Teaching Hospital ◽  
Parvovirus B19 ◽  
Epidemiological Data ◽  
Antenatal Clinic ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Parvovirus B19 ◽  
Igm Antibodies

Background: Human Parvovirus B19 (B19V) is a DNA virus, transmitted through respiratory secretions, hand-to-mouth-contact, blood transfusion and trans-placental transfer. It causes high mortality and morbidity in pregnant women, thus contributing to poor maternal and child health. B19V has been neglected due to dearth of epidemiological data. The aim of this study was to determine the sero-prevalence of Human Parvovirus B19 antibodies among pregnant women attending antenatal clinic at Federal Teaching Hospital Ido-Ekiti, Nigeria. Materials and Methods: This cross-sectional study enrolled pregnant women attending Federal Teaching Hospital Ido-Ekiti from January to May 2019 to obtain sero-epidemiological data. One hundred and twenty-two (122) consenting pregnant women were enrolled following institutional ethical approval. They were administered structured questionnaire and venous blood was collected in plain tubes for serum extraction. Sera samples were analyzed for IgG and IgM antibodies using the enzyme linked immunosorbent assay method. Percentages, median, chi-square and multivariate analysis were carried out using SPSS version 17. Results: The prevalence of IgG was 44.3% (54/122), IgM 41.8% (51/122), and IgG/IgM 28.7% (35/122) leaving 55.7% (68/122) of the population uninfected. The median age of participants was 22 (Interquartile range 18-25) years among which 36-45years had the highest prevalence which was not statistically significant (p=0.09 4.75). There was association between miscarriage, still birth, history of blood transfusion and prevalence of Human Parvovirus B19 (p<0.05). Conclusion: There is a high Prevalence of B19V among pregnant women attending antenatal clinic in this study. This underscores the need for testing and immunization of pregnant women against B19V.

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