Nucleotide sequence of the entire protein coding region of canine distemper virus polymerase-associated (P) protein mRNA

1985 ◽  
Vol 3 (4) ◽  
pp. 367-372 ◽  
Author(s):  
T. Barrett ◽  
S.B. Shrimpton ◽  
S.E.H. Russell
Keyword(s):  
Nucleotide Sequence ◽  
Canine Distemper Virus ◽  
Canine Distemper ◽  
Coding Region ◽  
Protein Coding ◽  
P Protein

scholarly journals Canine distemper virus detection in asymptomatic and non vaccinated dogs

2010 ◽  
Vol 30 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Helen L. Del Puerto ◽  
Anilton C. Vasconcelos ◽  
Luciana Moro ◽  
Fabiana Alves ◽  
Gissandra F. Braz ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Canine Distemper Virus ◽  
Early Stage ◽  
Virus Detection ◽  
Vero Cells ◽  
Canine Distemper ◽  
Coding Region ◽  
Protein Coding ◽  
Dissociation Curves

A quantitative real time polymerase chain reaction (PCR) revealed canine distemper virus presence in peripheral blood samples from asymptomatic and non vaccinated dogs. Samples from eleven domestic dogs with no signs of canine distemper and not vaccinated at the month of collection were used. Canine distemper virus vaccine samples in VERO cells were used as positive controls. RNA was isolated with Trizol®, and treated with a TURBO DNA-free kit. Primers were designed for canine distemper virus nucleocapsid protein coding region fragment amplification (84 bp). Canine b-actin (93 bp) was utilized as the endogenous control for normalization. Quantitative results of real time PCR generated by ABI Prism 7000 SDS Software showed that 54.5% of dogs with asymptomatic canine distemper were positive for canine distemper virus. Dissociation curves confirmed the specificity of the real time PCR fragments. This technique could detect even a few copies of viral RNA and identificate subclinically infected dogs providing accurate diagnosis of this disease at an early stage.

Nucleotide sequence of the coat protein coding region of the potyvirus tobacco vein-banding mosaic virus

1994 ◽  
Vol 138 (1-2) ◽  
pp. 17-25 ◽  
Author(s):  
B. -Y. Chang ◽  
C. R. Huang ◽  
S. -D. Yeh ◽  
J. -K. Chiang ◽  
L. -M. Hung ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Coding Region ◽  
Protein Coding

scholarly journals Phosphorylation of Canine Distemper Virus P Protein by Protein Kinase C-ζ and Casein Kinase II

Virology ◽  
1997 ◽  
Vol 232 (1) ◽  
pp. 198-206 ◽  
Author(s):  
Zheng Liu ◽  
Clayton C. Huntley ◽  
Bishnu P. De ◽  
Tapas Das ◽  
Amiya K. Banerjee ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Canine Distemper Virus ◽  
Casein Kinase Ii ◽  
Casein Kinase ◽  
Canine Distemper ◽  
Kinase C ◽  
P Protein

scholarly journals The complete sequence of the mouse skeletal alpha-actin gene reveals several conserved and inverted repeat sequences outside of the protein-coding region.

1986 ◽  
Vol 6 (1) ◽  
pp. 15-25 ◽  
Author(s):  
M C Hu ◽  
S B Sharp ◽  
N Davidson
Keyword(s):  
Amino Acids ◽  
Nucleotide Sequence ◽  
Inverted Repeat ◽  
Actin Gene ◽  
Coding Region ◽  
Specific Expression ◽  
Protein Coding ◽  
Repeat Sequences ◽  
Actin Genes ◽  
Alpha Actin

The complete nucleotide sequence of a genomic clone encoding the mouse skeletal alpha-actin gene has been determined. This single-copy gene codes for a protein identical in primary sequence to the rabbit skeletal alpha-actin. It has a large intron in the 5'-untranslated region 12 nucleotides upstream from the initiator ATG and five small introns in the coding region at codons specifying amino acids 41/42, 150, 204, 267, and 327/328. These intron positions are identical to those for the corresponding genes of chickens and rats. Similar to other skeletal alpha-actin genes, the nucleotide sequence codes for two amino acids, Met-Cys, preceding the known N-terminal Asp of the mature protein. Comparison of the nucleotide sequences of rat, mouse, chicken, and human skeletal muscle alpha-actin genes reveals conserved sequences (some not previously noted) outside of the protein-coding region. Furthermore, several inverted repeat sequences, partially within these conserved regions, have been identified. These sequences are not present in the vertebrate cytoskeletal beta-actin genes. The strong conservation of the inverted repeat sequences suggests that they may have a role in the tissue-specific expression of skeletal alpha-actin genes.

scholarly journals ‘Rescue’ of mini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins

2005 ◽  
Vol 86 (4) ◽  
pp. 1077-1081 ◽  
Author(s):  
D. D. Brown ◽  
F. M. Collins ◽  
W. P. Duprex ◽  
M. D. Baron ◽  
T. Barrett ◽  
...  
Keyword(s):  
Measles Virus ◽  
Canine Distemper Virus ◽  
Infectious Virus ◽  
Canine Distemper ◽  
Rinderpest Virus ◽  
P Protein ◽  
Level Of Activity ◽  
L Proteins ◽  
Helper Plasmids ◽  
Negative Sense

Chloramphenicol acetyltransferase (CAT)-expressing negative-sense mini-genomic constructs of measles virus (MV) and rinderpest virus (RPV) were rescued by standard technology with helper plasmids expressing the nucleocapsid (N), phospho- (P) and large (L) proteins of MV, canine distemper virus (CDV) or RPV in order to determine whether the proteins of different viruses can function together. Homogeneous sets consisting of N, P and L plasmids derived from one virus were able to generate reporter gene expression from either mini-genomic construct. Heterogeneous sets of proteins from different viruses were not functional, with the exception that a low level of activity was obtained when MV N and P protein were combined with RPV L protein in the rescue of the MV mini-genomic construct, or CDV N was combined with RPV P and L in the rescue of the RPV mini-genome. However, only homogeneous sets of plasmids were able to rescue infectious virus from full-length anti-genome-expressing plasmids.

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