New peptide containing T-helper cell epitope of foot-and-mouth-disease virus and optionally B-lymphocyte epitope, useful in vaccine and for potentiating hormone activity

Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals that has caused tremendous economic losses worldwide. In this study, we designed a chimeric nanoparticles (CNPs) vaccine that displays the predominant epitope of the serotype O foot-and-mouth disease virus (FMDV) VP1 131-160 on the surface of MS2 phage. The recombinant protein was expressed inEscherichia Coliand can self-assemble into CNPs with diameter at 25–30 nmin vitro. A tandem repeat peptide epitopes (TRE) was prepared as control. Mice were immunized with CNPs, TRE and commercialized synthetic peptide vaccines (PepVac), respectively. The ELISA results showed that CNPs stimulated a little higher specific antibody levels to PepVac, but was significantly higher than the TRE groups. Moreover, the results from specific IFN-γ responses and lymphocyte proliferation test indicated that CNP immunized mice exhibited significantly enhanced cellular immune response compared to TRE. These results suggested that the CNPs constructed in current study could be a potential alternative vaccine in future FMDV control.

Download Full-text

Peer Review #1 of "Immunogenicity evaluation of MS2 phage-mediated chimeric nanoparticle displaying an immunodominant B cell epitope of foot-and-mouth disease virus (v0.1)"

10.7287/peerj.4823v0.1/reviews/1 ◽

2018 ◽

Author(s):

D Rowlands

Keyword(s):

B Cell ◽

Peer Review ◽

Disease Virus ◽

Cell Epitope ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

B Cell Epitope ◽

Mouth Disease Virus ◽

Foot And Mouth

Download Full-text

Inclusion of a specific T cell epitope increases the protection conferred against foot-and-mouth disease virus in pigs by a linear peptide containing an immunodominant B cell site

Virology Journal ◽

10.1186/1743-422x-9-66 ◽

2012 ◽

Vol 9 (1) ◽

pp. 66 ◽

Cited By ~ 13

Author(s):

Carolina Cubillos ◽

Beatriz G de la Torre ◽

Juan Bárcena ◽

David Andreu ◽

Francisco Sobrino ◽

...

Keyword(s):

T Cell ◽

B Cell ◽

Disease Virus ◽

Cell Epitope ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

T Cell Epitope ◽

Linear Peptide ◽

Mouth Disease Virus ◽

Foot And Mouth

Download Full-text

Expression and purification of a soluble B lymphocyte stimulator mutant modified with the T-helper cell epitope

Biotechnology Letters ◽

10.1007/s10529-006-9139-y ◽

2006 ◽

Vol 28 (20) ◽

pp. 1649-1654

Author(s):

Huiguang Gao ◽

Weiling Fu ◽

Rongfen Li ◽

Linfeng Chen ◽

Qing Ji ◽

...

Keyword(s):

B Lymphocyte ◽

Cell Epitope ◽

T Helper Cell ◽

Helper Cell ◽

T Helper ◽

Expression And Purification ◽

B Lymphocyte Stimulator

Download Full-text

A mutant of Asia 1 serotype of Foot-and-mouth disease virus with the deletion of an important antigenic epitope in the 3A protein

Canadian Journal of Microbiology ◽

10.1139/w10-112 ◽

2011 ◽

Vol 57 (3) ◽

pp. 169-176 ◽

Cited By ~ 2

Author(s):

Shuang Li ◽

Mingchun Gao ◽

Runxiang Zhang ◽

Ge Song ◽

Jun Song ◽

...

Keyword(s):

Disease Virus ◽

Nonstructural Protein ◽

Cell Epitope ◽

Foot And Mouth Disease ◽

Viral Disease ◽

In Vitro Transcription ◽

Mouth Disease ◽

Mouth Disease Virus ◽

Foot And Mouth

Foot-and-mouth disease is a highly contagious viral disease of cloven-hoofed animals. The availability of a vaccine for differentiating infected from vaccinated animals (DIVA) is crucial for the control and eradication of Foot-and-mouth disease virus (FMDV). Because traditional inactivated vaccines may contain trace nonstructural proteins interfering with the DIVA, we hypothesized that mutant FMDV with deletion of immunodominant epitopes may be valuable. Our previous study has generated a full-length cDNA clone (pBSAs) of FMDV serotype Asia 1 isolated in China. In this study, a B-cell epitope was identified in the 3A region of a nonstructural protein (NSP) by anti-FMDV cattle sera. Furthermore, we generated recombinant FMDV (rvAs-3A14D) by selectively deleting 14 amino acids (position 91–104) in the 3A region of the NSP. Following in vitro transcription and transfection in BHK-21 cells, we successfully rescued the rvAs-3A14D from BHK-21 cells. Characterization of the rvAs-3A14D revealed that the infectivity, antigenicity, and replication kinetics in BHK-21 cells and virulence in mice of the rvAs-3A14D were similar to that of its parent virus. Notably, the mutant rvAs-3A14D only replicated well in BHK-21 but did poorly in primary calf kidney cells. These data suggest that the recombinant FMDV with deletion of this epitope in the NSP may be potentially used as a candidate inactivated vaccine. Therefore, the application of the marker vaccine and differential diagnostic tests may open a promising new avenue for the development of a vaccine for DIVA.

Download Full-text

Salmonella Vaccine Vector System for Foot-and-Mouth Disease Virus and Evaluation of Its Efficacy with Virus-Like Particles

Vaccines ◽

10.3390/vaccines9010022 ◽

2021 ◽

Vol 9 (1) ◽

pp. 22

Author(s):

Yong Zhi ◽

Hyun Jung Ji ◽

Huichen Guo ◽

Jae Hyang Lim ◽

Eui-Baek Byun ◽

...

Keyword(s):

Disease Virus ◽

Cell Epitope ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Vector System ◽

Th2 Cells ◽

Virus Like Particles ◽

Mouth Disease Virus ◽

Foot And Mouth ◽

Ifn Γ

Foot-and-mouth disease virus (FMDV) causes a highly contagious and devastating disease in livestock animals and has a great potential to cause severe economic loss worldwide. The major antigen of FMDV capsid protein, VP1, contains the major B-cell epitope responsible for effectively eliciting protective humoral immunity. In this study, irradiated Salmonella Typhimurium (KST0666) were used as transgenic vectors containing stress-inducible plasmid pRECN-VP1 to deliver the VP1 protein from FMDV-type A/WH/CHA/09. Mice were orally inoculated with ATOMASal-L3 harboring pRECN-VP1, and FMDV virus-like particles, where (VLPFMDV)-specific humoral, mucosal, and cellular immune responses were evaluated. Mice vaccinated with attenuated Salmonella (KST0666) expressing VP1 (named KST0669) showed high levels of VLP-specific IgA in feces and IgG in serum, with high FMDV neutralization titer. Moreover, KST0669-vaccinated mice showed increased population of IFN-γ (type 1 T helper cells; Th1 cells)-, IL-5 (Th2 cells)-, and IL-17A (Th17 cells)-expressing CD4+ as well as activated CD8+ T cells (IFN-γ+CD8+ cells), detected by stimulating VLPFMDV. All data indicate that our Salmonella vector system successfully delivered FMDV VP1 to immune cells and that the humoral and cellular efficacy of the vaccine can be easily evaluated using VLPFMDV in a Biosafety Level I (BSL1) laboratory.

Download Full-text

Designing Functionally Versatile, Highly Immunogenic Peptide-Based Multiepitopic Vaccines against Foot-and-Mouth Disease Virus

Vaccines ◽

10.3390/vaccines8030406 ◽

2020 ◽

Vol 8 (3) ◽

pp. 406

Author(s):

Sira Defaus ◽

Mar Forner ◽

Rodrigo Cañas-Arranz ◽

Patricia de León ◽

María J. Bustos ◽

...

Keyword(s):

T Cell ◽

Disease Virus ◽

Animal Health ◽

Unmet Need ◽

Cell Epitope ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

T Cell Epitopes ◽

Mouth Disease Virus ◽

Foot And Mouth

A broadly protective and biosafe vaccine against foot-and-mouth disease virus (FMDV) remains an unmet need in the animal health sector. We have previously reported solid protection against serotype O FMDV afforded by dendrimeric peptide structures harboring virus-specific B- and T-cell epitopes, and also shown such type of multivalent presentations to be advantageous over simple B-T-epitope linear juxtaposition. Chemically, our vaccine platforms are modular constructions readily made from specified B- and T-cell epitope precursor peptides that are conjugated in solution. With the aim of developing an improved version of our formulations to be used for on-demand vaccine applications, we evaluate in this study a novel design for epitope presentation to the immune system based on a multiple antigen peptide (MAP) containing six immunologically relevant motifs arranged in dendrimeric fashion (named B2T-TB2). Interestingly, two B2T units fused tail-to-tail into a single homodimer platform elicited higher B- and T-cell specific responses than former candidates, with immunization scores remaining stable even after 4 months. Moreover, this macromolecular assembly shows consistent immune response in swine, the natural FMDV host, at reduced dose. Thus, our versatile, immunogenic prototype can find application in the development of peptide-based vaccine candidates for various therapeutic uses using safer and more efficacious vaccination regimens.

Download Full-text