Long-lasting T-cell reactivity to Mycobacterium leprae antigens in human volunteers vaccinated with killed M. leprae

Vaccine ◽  
1993 ◽  
Vol 11 (11) ◽  
pp. 1108-1112 ◽  
Author(s):  
Abu Salim Mustafa ◽  
Fredrik Oftung
Keyword(s):  
T Cell ◽  
Mycobacterium Leprae ◽  
Cell Reactivity ◽  
Human Volunteers ◽  
T Cell Reactivity

scholarly journals Recombinant polypeptide of Mycobacterium leprae as a potential tool for serological detection of leprosy

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Marcelo dos Santos Barbosa ◽  
Iara Beatriz Andrade de Sousa ◽  
Simone Simionatto ◽  
Sibele Borsuk ◽  
Silvana Beutinger Marchioro
Keyword(s):  
Large Scale ◽  
Mycobacterium Leprae ◽  
Detection Methods ◽  
Recombinant Polypeptide ◽  
Cell Reactivity ◽  
Tertiary Structures ◽  
Leprosy Patients ◽  
T Cell Reactivity ◽  
Prevention Methods ◽  
Scale Detection

AbstractCurrent prevention methods for the transmission of Mycobacterium leprae, the causative agent of leprosy, are inadequate as suggested by the rate of new leprosy cases reported. Simple large-scale detection methods for M. leprae infection are crucial for early detection of leprosy and disease control. The present study investigates the production and seroreactivity of a recombinant polypeptide composed of various M. leprae protein epitopes. The structural and physicochemical parameters of this construction were assessed using in silico tools. Parameters like subcellular localization, presence of signal peptide, primary, secondary, and tertiary structures, and 3D model were ascertained using several bioinformatics tools. The resultant purified recombinant polypeptide, designated rMLP15, is composed of 15 peptides from six selected M. leprae proteins (ML1358, ML2055, ML0885, ML1811, ML1812, and ML1214) that induce T cell reactivity in leprosy patients from different hyperendemic regions. Using rMLP15 as the antigen, sera from 24 positive patients and 14 healthy controls were evaluated for reactivity via ELISA. ELISA-rMLP15 was able to diagnose 79.17% of leprosy patients with a specificity of 92.86%. rMLP15 was also able to detect the multibacillary and paucibacillary patients in the same proportions, a desirable addition in the leprosy diagnosis. These results summarily indicate the utility of the recombinant protein rMLP15 in the diagnosis of leprosy and the future development of a viable screening test.

T-Cell Reactivity to GAD 65 in Insulin Dependent Diabetes

1995 ◽  
Vol 89 (s33) ◽  
pp. 14P-14P
Author(s):  
M Hawa ◽  
T Lohmann ◽  
M Londei ◽  
D Leslie
Keyword(s):  
T Cell ◽  
Insulin Dependent Diabetes ◽  
Cell Reactivity ◽  
Gad 65 ◽  
Insulin Dependent ◽  
T Cell Reactivity

scholarly journals 345 PERSISTENT HEPATITIS C VIRUS (HCV) INFECTION IMPAIRS HCV-SPECIFIC CYTOTOXIC T CELL REACTIVITY THROUGH Mcl-1/Bim IMBALANCE DUE TO CD127 DOWN-REGULATION

2013 ◽  
Vol 58 ◽  
pp. S143-S144
Author(s):  
J.R. Larrubia ◽  
M.U. Lokhande ◽  
S. García-Garzón ◽  
J. Miquel ◽  
A. González-Praetorious ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
T Cell ◽  
Hcv Infection ◽  
Cytotoxic T Cell ◽  
Down Regulation ◽  
Cell Reactivity ◽  
T Cell Reactivity

Association of T-cell reactivity with β-cell function in recent onset type 1 diabetes patients

2010 ◽  
Vol 34 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Christian Pfleger ◽  
Guido Meierhoff ◽  
Hubert Kolb ◽  
Nanette C. Schloot
Keyword(s):  
Type 1 Diabetes ◽  
T Cell ◽  
Cell Function ◽  
Β Cell ◽  
Cell Reactivity ◽  
Recent Onset ◽  
Onset Type ◽  
Β Cell Function ◽  
T Cell Reactivity

Cytotoxic T cell reactivity and HLA-B35 binding of the variantPlasmodium falciparum circumsporozoite protein CD8+ CTL epitope in naturally exposed Kenyan adults

1997 ◽  
Vol 27 (8) ◽  
pp. 1952-1957 ◽  
Author(s):  
Venkatachalam Udhayakumar ◽  
John M. Ongecha ◽  
Ya-Ping Shi ◽  
Michael Aidoo ◽  
A. S. S. Orago ◽  
...  
Keyword(s):  
T Cell ◽  
Circumsporozoite Protein ◽  
Cytotoxic T Cell ◽  
Cell Reactivity ◽  
Ctl Epitope ◽  
T Cell Reactivity

scholarly journals Age Distribution for T Cell Reactivity to Vaccinia Virus in a Healthy Population

2004 ◽  
Vol 38 (1) ◽  
pp. 86-89 ◽  
Author(s):  
Szu‐Min Hsieh ◽  
Sung‐Ching Pan ◽  
Shey‐Ying Chen ◽  
Pei‐Fang Huang ◽  
Shan‐Chwen Chang
Keyword(s):  
T Cell ◽  
Vaccinia Virus ◽  
Age Distribution ◽  
Healthy Population ◽  
Cell Reactivity ◽  
T Cell Reactivity

scholarly journals Diagnostic value of specific T cell reactivity to drugs in 95 cases of drug induced liver injury

Gut ◽  
1997 ◽  
Vol 41 (4) ◽  
pp. 534-540 ◽  
Author(s):  
V A J Maria ◽  
R M M Victorino
Keyword(s):  
T Cell ◽  
Liver Injury ◽  
Mononuclear Cells ◽  
Diagnostic Value ◽  
Drug Induced ◽  
Cell Reactivity ◽  
Peripheral Blood Mononuclear ◽  
Drug Induced Liver Injury ◽  
Prostaglandin Inhibitor ◽  
T Cell Reactivity

Background—Diagnosis of drug induced liver injury is usually based on a temporal relation between drug intake and clinical picture as well as on the exclusion of alternative causes. More precise diagnosis has been attempted by using in vitro specific T cell reactivity to drugs but the test has never reached general acceptability because of frequent negative results which could be explained, in part, by prostaglandin producing suppressor cells (PPSC).Aim—To analyse the diagnostic value of a modified test where lymphocyte responses to drugs are detected in the presence of a prostaglandin inhibitor.Patients—Ninety five patients with a clinical diagnosis of drug induced liver injury, 106 healthy controls, 35 individuals with recent exposure to the same drugs without adverse effects, and 15 patients with liver disease unrelated to drugs.Methods—Peripheral blood mononuclear cells (PBMC) were cultured in the presence of drugs alone and in the presence of drugs and a prostaglandin inhibitor. Responses were assessed by3H-thymidine incorporation in lymphocytes. Results were expressed as counts per minute and as stimulation indexes (SI).Results—When PBMC were stimulated with drugs alone, lymphocyte sensitisation to drugs (SI>2) was detected in 26% of the cases. This was noticeably increased (56%) when a prostaglandin inhibitor was added to the cultures. No reactivity was found in controls. In patients with possible sensitivity to several drugs, lymphocyte reactivity was detected to only one drug. The severity of the lesions, as assessed by aminotransferase concentrations and disease duration, was lower in patients with evidence of PPSC.Conclusions—This new approach is useful for the diagnosis of drug induced liver injury, particularly in patients exposed to more than one drug; furthermore, the presence of putative PPSC is associated with less severe forms of drug induced hepatitis.

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