Epstein-Barr virus DNA detection in gingival tissues of patients undergoing surgical extractions

Objectives: Epstein–Barr virus is a tumorigenic virus and has been extensively studied as a causative agent for Hodgkin lymphoma. Although immunostaining of the tumor biopsy is the standard method for diagnosis of Epstein–Barr virus-driven Hodgkin lymphoma, the invasiveness of the procedure renders it difficult and less desirable for the patients. Therefore, we designed this study to evaluate the efficiency of plasma Epstein–Barr virus DNA detection as an alternative diagnostic and prognostic method for Epstein–Barr virus–associated Hodgkin lymphoma. Methods: This analytical cross-sectional study was conducted during March 2017 to December 2018 including 43 Hodgkin lymphoma patients diagnosed histopathologically followed by the latent membrane protein-1 immunohistochemistry to determine their Epstein–Barr virus association. Plasma Epstein–Barr virus DNA in these samples was measured using quantitative polymerase chain reaction (qPCR). Results: Of total, 29 (67.44%) patients tested positive for plasma Epstein–Barr virus DNA. On comparing results of latent membrane protein-1 immunohistochemistry (IHC) with plasma Epstein–Barr virus DNA, plasma Epstein–Barr virus DNA was found in 25 of 30 patients with latent membrane protein-1 expression and 4 of 13 patients without latent membrane protein-1 expression. The sensitivity and the specificity of plasma Epstein–Barr virus DNA detection with respect to latent membrane protein-1 IHC were found to be 83.33% and 69.23%, respectively ( p = 0.0014). Conclusion: Determination of plasma Epstein–Barr virus DNA was found to be highly sensitive and specific in characterizing Epstein–Barr virus–associated Hodgkin lymphoma, suggesting that this diagnostic method holds promise as an alternative and more convenient method of diagnosis compared with tissue biopsy.

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Positive Predictive Value of Epstein-Barr Virus DNA Detection in HIV-Related Primary Central Nervous System Lymphoma

Clinical Infectious Diseases ◽

10.1086/425132 ◽

2004 ◽

Vol 39 (9) ◽

pp. 1396-1397 ◽

Cited By ~ 5

Author(s):

P. Cinque ◽

A. Cingolani ◽

S. Bossolasco ◽

A. Antinori

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Positive Predictive Value ◽

Predictive Value ◽

Epstein Barr Virus ◽

Dna Detection ◽

Central Nervous System Lymphoma ◽

Barr Virus ◽

Epstein Barr

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DNA detection with PCR (11th) Epstein-Barr virus in paraffin-embedded tissue

Japanese Journal of Radiological Technology ◽

10.6009/jjrt.kj00001353992 ◽

1996 ◽

Vol 52 (10) ◽

pp. 1531

Author(s):

Takashi Arai ◽

Hideaki Yamamoto ◽

Nobuo Hirosawa ◽

Hideyuki Kojima ◽

Katsumi Ikei

Keyword(s):

Epstein Barr Virus ◽

Dna Detection ◽

Barr Virus ◽

Epstein Barr

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Epstein-Barr Virus Infection Is Predictive of CNS Involvement in Systemic AIDS-Related Non-Hodgkin’s Lymphomas

Journal of Clinical Oncology ◽

10.1200/jco.2000.18.19.3325 ◽

2000 ◽

Vol 18 (19) ◽

pp. 3325-3330 ◽

Cited By ~ 69

Author(s):

Antonella Cingolani ◽

Roberta Gastaldi ◽

Lucia Fassone ◽

Francesco Pierconti ◽

Maria Letizia Giancola ◽

...

Keyword(s):

Epstein Barr Virus ◽

Dna Detection ◽

Molecular Heterogeneity ◽

Cns Involvement ◽

Predictive Values ◽

Barr Virus ◽

Ebv Infection ◽

Ebv Dna ◽

Epstein Barr ◽

Hodgkin's Lymphomas

PURPOSE: This study aimed at correlating Epstein-Barr virus (EBV) infection of systemic AIDS-related non-Hodgkin lymphomas (AIDS-NHL) with the development of a CNS localization of the tumor. PATIENTS AND METHODS: Demographic, epidemiologic, clinical, histologic, and virologic features were collected for all systemic AIDS-NHL patients included in the study (n = 50). Pathologic specimens were classified according to the working formulation for NHL and the Revised European-American Lymphoma classification. EBV infection in tumor tissue samples was studied by EBV small encoded RNA in situ hybridization; EBV-DNA detection in CSF was carried out by nested polymerase chain reaction using Epstein-Barr nuclear antigen-1–specific primers. In addition, selected EBV-positive lymphomas were subjected to a detailed characterization of EBV molecular heterogeneity. RESULTS: Eleven patients had a CNS involvement at some point during their clinical history (four at diagnosis and seven at relapse). Thirty patients (11 with CNS involvement and 19 without) harbored EBV infection of the tumor. Sensitivity, specificity, and positive and negative predictive values of EBV-DNA detection in CSF for CNS involvement by lymphoma were 90%, 100%, 100%, and 97.6%, respectively. Factors significantly predictive of CNS involvement were EBV infection of the tumor (P = .003), an extranodal disease at diagnosis other than CNS (P = .006), and a non-CNS relapse (P = .01). In four cases of CNS involvement, EBV-DNA in CSF preceded any other sign of disease by a mean of 35 days. CONCLUSION: These results show that EBV infection of the tumor clone significantly increases the risk of CNS involvement by systemic AIDS-NHL, without regard of specific molecular features. The detection of EBV-DNA in the CSF of AIDS-NHL patients may select cases with higher risk of CNS involvement and, therefore, may prove useful in the therapeutic stratification of these tumors.

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Use of transoral nasopharyngeal brush biopsy for Epstein-Barr virus DNA detection of local recurrence of nasopharyngeal carcinoma after radiotherapy

Head & Neck ◽

10.1002/hed.24216 ◽

2015 ◽

Vol 38 (S1) ◽

pp. E1301-E1304 ◽

Cited By ~ 9

Author(s):

Jacky Wai-Kei Lam ◽

Jimmy Yu-Wai Chan ◽

Wai-Kuen Ho ◽

Raymond King-Yin Tsang

Keyword(s):

Nasopharyngeal Carcinoma ◽

Local Recurrence ◽

Epstein Barr Virus ◽

Dna Detection ◽

Brush Biopsy ◽

Barr Virus ◽

Epstein Barr

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496. DNA detection of Epstein-Barr virus with PCR : EBV DNA in peripheral blood

Japanese Journal of Radiological Technology ◽

10.6009/jjrt.kj00003326295 ◽

1994 ◽

Vol 50 (8) ◽

pp. 1411

Author(s):

Takashi Arai ◽

Hideaki Yamamoto ◽

Satoshi Kobayashi ◽

Hideyuki Kojima ◽

Katsumi Ikei

Keyword(s):

Peripheral Blood ◽

Epstein Barr Virus ◽

Dna Detection ◽

Barr Virus ◽

Ebv Dna ◽

Epstein Barr

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A Quantitative Ultrastructural Study of Peripheral Blood Lymphocytes Containing Parallel Tubular Arrays in Epstein-Barr Virus and Cytomegalo-Virus Mononucleosis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100098769 ◽

1981 ◽

Vol 39 ◽

pp. 454-455

Author(s):

C. M. Payne ◽

P. M. Tennican

Keyword(s):

Peripheral Blood ◽

Lymphocyte Count ◽

Epstein Barr Virus ◽

Absolute Lymphocyte Count ◽

Peripheral Blood Lymphocytes ◽

Clinical State ◽

Barr Virus ◽

The Absolute ◽

Epstein Barr ◽

Tubular Arrays

In the normal peripheral circulation there exists a sub-population of lymphocytes which is ultrastructurally distinct. This lymphocyte is identified under the electron microscope by the presence of cytoplasmic microtubular-like inclusions called parallel tubular arrays (PTA) (Figure 1), and contains Fc-receptors for cytophilic antibody. In this study, lymphocytes containing PTA (PTA-lymphocytes) were quantitated from serial peripheral blood specimens obtained from two patients with Epstein -Barr Virus mononucleosis and two patients with cytomegalovirus mononucleosis. This data was then correlated with the clinical state of the patient.It was determined that both the percentage and absolute number of PTA- lymphocytes was highest during the acute phase of the illness. In follow-up specimens, three of the four patients' absolute lymphocyte count fell to within normal limits before the absolute PTA-lymphocyte count.In one patient who was followed for almost a year, the absolute PTA- lymphocyte count was consistently elevated (Figure 2). The estimation of absolute PTA-lymphocyte counts was determined to be valid after a morphometric analysis of the cellular areas occupied by PTA during the acute and convalescent phases of the disease revealed no statistical differences.

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Studies of Epstein-Barr Virus Antigens Using Immunoperoxidase and Immunofluorescence Techniques

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010011533x ◽

1975 ◽

Vol 33 ◽

pp. 342-343

Author(s):

R. Stephens ◽

K. Traul ◽

D. Woolf ◽

P. Gaudreau

Keyword(s):

Electron Microscopy ◽

Nasopharyngeal Carcinoma ◽

Epstein Barr Virus ◽

Barr Virus ◽

If Technique ◽

Infected Cells ◽

Virus Antigens ◽

Epstein Barr ◽

Virus Capsid Antigen ◽

Antigen Reactivity

A number of antigens have been found associated with persistent EBV infections of lymphoblastoid cells. Identification and localization of these antigens were principally by immunofluorescence (IF) techniques using sera from patients with nasopharyngeal carcinoma (NPC), Burkitt lymphoma (BL), and infectious mononucleosis (IM). Our study was mainly with three of the EBV related antigens, a) virus capsid antigen (VCA), b) membrane antigen (MA), and c) early antigens (EA) using immunoperoxidase (IP) techniques with electron microscopy (EM) to elucidate the sites of reactivity with EBV and EBV infected cells.Prior to labeling with horseradish peroxidase (HRP), sera from NPC, IM, and BL cases were characterized for various reactivities by the indirect IF technique. Modifications of the direct IP procedure described by Shabo and the indirect IP procedure of Leduc were made to enhance penetration of the cells and preservation of antigen reactivity.

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