Ciliary neurotrophic factor supports target-deprived preganglionic sympathetic spinal cord neurons

1989 ◽  
Vol 105 (3) ◽  
pp. 316-320 ◽  
Author(s):  
Dieter Blottner ◽  
Wolfgang Brüggemann ◽  
Klaus Unsicker
Keyword(s):  
Spinal Cord ◽  
Neurotrophic Factor ◽  
Ciliary Neurotrophic Factor ◽  
Spinal Cord Neurons

Survival in vitro of motoneurons identified or purified by novel antibody-based methods is selectively enhanced by muscle-derived factors

Development ◽  
1991 ◽  
Vol 111 (1) ◽  
pp. 221-232 ◽  
Author(s):  
E. Bloch-Gallego ◽  
M. Huchet ◽  
H. el M'Hamdi ◽  
F.K. Xie ◽  
H. Tanaka ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Monoclonal Antibody ◽  
Growth Factors ◽  
Neurotrophic Factor ◽  
Ciliary Neurotrophic Factor ◽  
Floor Plate ◽  
New Method ◽  
Motoneuron Survival ◽  
Muscle Extract

Motoneurons were identified in vitro by a new method using the SC1 monoclonal antibody. They constituted up to 30% of total neurons in cultures of whole spinal cord from 4.5-day chicken embryos, and survived for at least 5 days in the presence of muscle extract, but not in its absence. By contrast, other neurons and floor-plate cells survived without muscle-derived factors. Motoneurons were purified to homogeneity by ‘panning’ on dishes coated with SC1 antibody; they developed rapidly even in the absence of other spinal cells. Concentrations of muscle extract required for half-maximal motoneuron survival were indistinguishable in pure and mixed cultures, suggesting that muscle-derived factors act directly on motoneurons. Other purified growth factors tested, including ciliary neurotrophic factor, did not have the survival-promoting activity of muscle.

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