Fractionation of ovalbumin glycopeptide AC-C by high-pressure liquid chromatography Determination of structure by 1H-NMR spectroscopy

1983 ◽  
Vol 759 (3) ◽  
pp. 214-221 ◽  
Author(s):  
C CECCARINI ◽  
P LORENZONI ◽  
P ATKINSON
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
High Pressure Liquid Chromatography ◽  
1H Nmr Spectroscopy

A Study of the Metabolome of Ricinus communis for Forensic Applications

2010 ◽  
Vol 63 (1) ◽  
pp. 8 ◽  
Author(s):  
Simon P. B. Ovenden ◽  
Benjamin R. Gordon ◽  
Christina K. Bagas ◽  
Bob Muir ◽  
Simone Rochfort ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
East Coast ◽  
Ricinus Communis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Nmr Data

Investigations were undertaken to ascertain the appropriateness of studying the metabolome of Ricinus communis for cultivar and provenance determination. Seeds from 14 R. communis specimens (a total of 56 seeds) collected from the east coast of Australia were analyzed by high pressure liquid chromatography with UV detection (HPLC-UV), liquid chromatography–mass spectrometry (LC-MS), and 1H NMR spectroscopy. The collected data were then analyzed using principle component analysis (PCA). For HPLC-UV analysis, six R. communis specimens were unambiguously identified by PCA as belonging to separate classes relating to specimen. LC-MS data allowed unique ions to be identified for four specimens. Conversely 10 specimens were unambiguously segregated in the PCA of the 1H NMR data. The ratio of ricinine 1 to demethylricinine analogues 2 and 3 was found to be important for specimen determination. These combined analyses suggested that a combination of HPLC-UV and 1H NMR in conjunction with PCA could allow for specimen differentiation.

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