Ether-water partitioning and permeability through nude mouse skin in vitro. I. Urea, thiourea, glycerol and glucose

A class of 1-((benzo[d]thiazol-2-ylamino)(phenyl)methyl)naphthalen-2-ol derivatives (4a-t) has been synthesized in good yields through a three component coupling reaction. The newly synthesized compounds were evaluated for their in vitro antiproliferative activity against five cell lines such as DU145 (human prostate cancer), MDA-MB-B231 (human breast cancer), SKOV3 (human ovarian cancer), B16-F10 (mouse skin melanoma) and CHO-K1 (Chinese hamster ovary cells), a noncancerous cell line. In vitro inhibitory activity indicates that compounds 4a, 4b, 4c, 4d, 4g, 4j, and 4o exhibited potent anti-proliferative behavior. Among them, compounds 4g, 4j and 4o found to be the most active members exhibiting remarkable growth inhibitory activity. Molecular docking facilitates to investigate the probable binding mode and key active site interactions in tubulins α and β proteins. The docking results are complementary to experimental results.

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Immature rat seminiferous tubules reconstructed in vitro express markers of Sertoli cell maturation after xenografting into nude mouse hosts

MHR Basic science of reproductive medicine ◽

10.1093/molehr/gap081 ◽

2009 ◽

Vol 16 (2) ◽

pp. 97-110 ◽

Cited By ~ 19

Author(s):

K. Gassei ◽

J. Ehmcke ◽

M.A. Wood ◽

W.H. Walker ◽

S. Schlatt

Keyword(s):

Sertoli Cell ◽

Nude Mouse ◽

Cell Maturation ◽

Seminiferous Tubules ◽

Immature Rat

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Chromatic intervention and biocompatibility assay for biosurfactant derived from Balanites aegyptiaca (L.) Del

Scientific Reports ◽

10.1038/s41598-021-83573-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Vishal Panchariya ◽

Vishal Bhati ◽

Harishkumar Madhyastha ◽

Radha Madhyastha ◽

Jagdish Prasad ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Skin Fibroblast ◽

Mouse Skin ◽

Fibroblast Cells ◽

Toxicity Assay ◽

Balanites Aegyptiaca ◽

Hemolysis Assay ◽

Full Factorial ◽

Human Rbcs

AbstractExtraction of biosurfactants from plants is advantageous than from microbes. The properties and robustness of biosurfactant derived from the mesocarp of Balanites aegyptiaca have been reported. However, the dark brown property of biosurfactant and lack of knowledge of its biocompatibility limits its scope. In the present work, the decolorization protocol for this biosurfactant was optimized using hydrogen peroxide. The hemolytic potential and biocompatibility based on cell toxicity and proliferation were also investigated. This study is the first report on the decolorization and toxicity assay of this biosurfactant. For decolorization of biosurfactant, 34 full factorial design was used, and the data were subjected to ANOVA. Results indicate that 1.5% of hydrogen peroxide can decolorize the biosurfactant most efficiently at 40 °C in 70 min at pH 7. Mitochondrial reductase (MTT) and reactive oxygen species (ROS) assays on M5S mouse skin fibroblast cells revealed that decolorized biosurfactant up to 50 µg/mL for 6 h had no significant toxic effect. Hemolysis assay showed ~ 2.5% hemolysis of human RBCs, indicating the nontoxic effect of this biosurfactant. The present work established a decolorization protocol making the biosurfactant chromatically acceptable. Biocompatibility assays confirm its safer use as observed by experiments on M5S skin fibroblast cells under in vitro conditions.

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The Nude Mouse Skin Phenotype: The Role of Foxn1 in Hair Follicle Development and Cycling

Experimental and Molecular Pathology ◽

10.1006/exmp.2001.2386 ◽

2001 ◽

Vol 71 (2) ◽

pp. 171-178 ◽

Cited By ~ 51

Author(s):

Lars Mecklenburg ◽

Motonobu Nakamura ◽

John P. Sundberg ◽

Ralf Paus

Keyword(s):

Hair Follicle ◽

Nude Mouse ◽

Mouse Skin ◽

Follicle Development ◽

Hair Follicle Development

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Resinless section immunogold electron microscopy of karyo-cytoskeletal frameworks of eukaryotic cells cultured in vitro. Absence of a salt-stable nuclear matrix from mouse plasmacytoma MPC-11 cells

Journal of Cell Science ◽

10.1242/jcs.98.1.107 ◽

1991 ◽

Vol 98 (1) ◽

pp. 107-122

Author(s):

X. Wang ◽

P. Traub

Keyword(s):

Electron Microscopy ◽

Nuclear Matrix ◽

Protein A ◽

Mouse Skin ◽

Nuclear Lamina ◽

Immunogold Electron Microscopy ◽

Cell Structures ◽

Critical Point Drying ◽

Cells Cultured

The karyo-cytoskeleton of cells cultured in vitro was investigated employing resinless section immunogold electron microscopy. Cells were entrapped in low-melting agarose, sequentially extracted with various buffers and digested with nucleases to obtain karyo-cytoskeletal frameworks and reacted with specific primary and gold-conjugated secondary antibodies or gold-conjugated protein A to decorate structural elements of these frameworks. Following embedment of the gold-labeled residual cell structures in diethylene glycol distearate and their sectioning, the embedding material was removed with organic solvent and the sections were finally subjected to CO2 critical point drying. When this technique was applied to mouse skin fibroblasts (MSF), it revealed a dense and salt-stable intranuclear network of fibrogranular material. Antibodies directed against vimentin and lamin B detected a cytoplasmic meshwork of intermediate filaments (IFs) and a nuclear lamina, respectively; the latter, however, only after removal of chromatin from nuclei by nuclease digestion of DNA. Intranuclear filaments free of adhering globular material were morphologically very similar to cytoplasmic vimentin filaments. By contrast, mouse plasmacytoma MPC-11 cells lacking detectable amounts of cytoplasmic IF proteins and lamins A and C were devoid of a salt-stable internal nuclear matrix. The same holds true for MPC-11 cells that had been treated with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate to induce vimentin synthesis and establish a cytoplasmically extended IF network. These findings were in accordance with the biochemical behavior of Triton X-100-treated MSF and MPC-11 cells and their appearance in immunofluorescence microscopy upon extraction with high ionic strength buffer. While the chromatin was quantitatively retained in the residual cell structures derived from MSF cells, in those obtained from MPC-11 cells the nuclear lamina was disrupted and the chromatin was released from the nuclei, suggesting that MPC-11 cells lack the salt-stable nuclear scaffold to which chromatin is normally anchored.

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