Antiamoebic activity of Piper longum fruits against Entamoeba histolytica in vitro and in vivo

1996 ◽  
Vol 50 (3) ◽  
pp. 167-170 ◽  
Author(s):  
Sheela Ghoshal ◽  
B.N.Krishna Prasad ◽  
V. Lakshmi
Keyword(s):  
Entamoeba Histolytica ◽  
Piper Longum ◽  
Antiamoebic Activity

scholarly journals In vitro and in vivo Antiamoebic Potential of Actinopyga lecanora (Jaeger)

2015 ◽  
Vol 18 (2) ◽  
pp. 118-120 ◽  
Author(s):  
Vijai Lakshmi ◽  
Sheela Ghosal
Keyword(s):  
Entamoeba Histolytica ◽  
Symptomatic Relief ◽  
Pharmaceutical Journal ◽  
Climatic Conditions ◽  
Isolation And Characterization ◽  
Histolytica Infection ◽  
Antiamoebic Activity

Human amoebiasis, due to Entamoeba histolytica infection, is mainly associated with morbidity thus affecting the quality of life and pace of development in the countries with warm climatic conditions. So far, the available drugs provide only symptomatic relief and they are not devoid of side effects. This leads to obtain novel molecules from natural sources having antiamoebic activity. The methanol extract of Actinopyga lecanora (Jaeger) displayed antiamoebic activity. It showed MIC 125 ?g/ml in our in-vitro studies, but when it was tested in rats, it revealed 88% inhibition of trophozoites at the dose of 900 mg/kg body weight against Entamoeba histolytica. Further work is in progress for the isolation and characterization of active molecules.Bangladesh Pharmaceutical Journal 18(2): 118-120, 2015

scholarly journals Antiprotozoal Activity Against Entamoeba histolytica of Flavonoids Isolated from Lippia graveolens Kunth

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2464
Author(s):  
Ramiro Quintanilla-Licea ◽  
Javier Vargas-Villarreal ◽  
María Julia Verde-Star ◽  
Verónica Mayela Rivas-Galindo ◽  
Ángel David Torres-Hernández
Keyword(s):  
Entamoeba Histolytica ◽  
Inhibitory Concentration ◽  
Public Health Problem ◽  
In Vitro Tests ◽  
Antiprotozoal Activity ◽  
Subsequent Work ◽  
Antiamoebic Activity ◽  
Work Up ◽  
First Time

Amebiasis caused by Entamoeba histolytica is nowadays a serious public health problem worldwide, especially in developing countries. Annually, up to 100,000 deaths occur across the world. Due to the resistance that pathogenic protozoa exhibit against commercial antiprotozoal drugs, a growing emphasis has been placed on plants used in traditional medicine to discover new antiparasitics. Previously, we reported the in vitro antiamoebic activity of a methanolic extract of Lippia graveolens Kunth (Mexican oregano). In this study, we outline the isolation and structure elucidation of antiamoebic compounds occurring in this plant. The subsequent work-up of this methanol extract by bioguided isolation using several chromatographic techniques yielded the flavonoids pinocembrin (1), sakuranetin (2), cirsimaritin (3), and naringenin (4). Structural elucidation of the isolated compounds was achieved by spectroscopic/spectrometric analyses and comparing literature data. These compounds revealed significant antiprotozoal activity against E. histolytica trophozoites using in vitro tests, showing a 50% inhibitory concentration (IC50) ranging from 28 to 154 µg/mL. Amebicide activity of sakuranetin and cirsimaritin is reported for the first time in this study. These research data may help to corroborate the use of this plant in traditional Mexican medicine for the treatment of dyspepsia.

Entamoeba histolytica: Chemoattractant activity for gerbil neutrophils in vivo and in vitro

1987 ◽  
Vol 64 (1) ◽  
pp. 12-23 ◽  
Author(s):  
K. Chadee ◽  
France Moreau ◽  
E. Meerovitch
Keyword(s):  
Entamoeba Histolytica

scholarly journals In Vitro Study of Antiamoebic Activity of Methanol Extracts ofArgemone mexicanaon Trophozoites ofEntamoeba histolyticaHM1-IMSS

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Joel H. Elizondo-Luévano ◽  
Rocío Castro-Ríos ◽  
Eduardo Sánchez-García ◽  
Magda E. Hernández-García ◽  
Javier Vargas-Villarreal ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Methanolic Extract ◽  
Parasitic Infection ◽  
In Vitro Study ◽  
Argemone Mexicana ◽  
Traditional Use ◽  
Antiparasitic Activity ◽  
Antiamoebic Activity ◽  
Amoebicidal Activity

Infections caused by parasites in humans represent one of the main public health concerns. Amoebiasis, a parasitic infection caused byEntamoeba histolytica(E. histolytica), is considered endemic in Mexico, whereArgemone mexicana(A. mexicana) has been used in traditional medicine to treat intestinal parasitic diseases. The objective of this work was to evaluate the potential biological activity ofA. mexicanaonE. histolytica. For this purpose, a methanolic extract was prepared fromA. mexicanaleaves, and a differential fractionation was carried out with solvents of different polarities. The inhibitory capacities of the extract and its fractions were evaluated in vitro using HM1-IMSS, a strain ofEntamoeba histolytica.A. mexicanaextract was found to have a growth-inhibiting activity forE. histolytica, showing IC50 = 78.39 μg/mL. The extract was characterized phytochemically, and the methanolic extract fractions were analyzed by liquid chromatography (HPLC) and mass spectrometry (MS). Berberine and jatrorrhizine were present in the active fractions, and these compounds may be responsible for the antiparasitic activity. The identification of amoebicidal activity ofA. mexicanaonE. histolyticagives support to the traditional use. Further studies with berberine and jatrorrhizine will be carried out to understand the mechanism involved.

scholarly journals Activity of Extracts and Procesterol from Calotropis gigantea against Entamoeba histolytica

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Shailendra Singh ◽  
Neelam Bharti ◽  
Manoj Chugh ◽  
Fehmida Naqvi ◽  
Amir Azam
Keyword(s):  
Entamoeba Histolytica ◽  
Chromatographic Separation ◽  
Chloroform Extract ◽  
Root Bark ◽  
Inhibitory Effects ◽  
Calotropis Gigantea ◽  
Growth Inhibitory ◽  
Antiamoebic Activity

Extracts from the root bark of Calotropis gigantea were subjected to bioactivity-guided fractionation using growth inhibitory effects against Entamoeba histolytica. The n-hexane soluble portion of the chloroform extract showed in vitro antiamoebic activity against the HK-9 strain of Entamoeba histolytica. Chromatographic separation of the chloroform extract afforded the known compound, procesterol, which showed activity against E. histolytica.

Entamoeba histolytica: The over expression of a mutated EhRabB protein produces a decrease of in vitro and in vivo virulence

2013 ◽  
Vol 133 (3) ◽  
pp. 339-345 ◽  
Author(s):  
L.J. Juárez-Hernández ◽  
R.M. García-Pérez ◽  
A. Salas-Casas ◽  
G. García-Rivera ◽  
E. Orozco ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Over Expression

scholarly journals Obtención de anticuerpos monoclonales de ratón contra proteasa de cisteína 5 recombinante de Entamoeba histolytica

2012 ◽  
pp. 3014-3023
Author(s):  
Juanita Trejos S ◽  
Jhon Castaño O
Keyword(s):  
Entamoeba Histolytica ◽  
Entamoeba Dispar

RESUMENObjetivo. Obtener anticuerpos monoclonales de ratón contra la proteasas de cisteína 5 (EhCP5) de Entamoeba histolytica. Materiales y métodos. Se inmunizaron ratones BALB/c por vía intraperitoneal con adyuvante de Freund completo e incompleto con la proteína recombinante EhCP5 obtenida a partir del cultivo de E.coli DH5α trasfectada con el vector recombinante pJC45 que expresa dicha proteína. Se seleccionó el animal con mejor respuesta de anticuerpos. Al cual se le extrajo su bazo como fuente de linfocitos B, los cuales se fusionaron utilizando PEG con células de mieloma de ratón SP2-0/Ag14. Se procedió a selección de los hibridomas y a la evaluación de los sobrenadantes de las colonias que crecieron a los 7 días mediante ELISA. Los hibridomas con valores más altos de anticuerpos específicos contra la proteína EhCP5r se seleccionaron, y los clones obtenidos por diluciones limitantes fueron expandidos. Resultados. A partir de un clon secretor estable se purifico el anticuerpo monoclonal anti EhCP5r del isotipo IgG1 por cromatografía de afinidad con proteína G. Los clones fueron expandidos in vivo e in vitro. Con el anticuerpo purificado se diseñaron tres sistemas de captura para evaluar la aplicabilidad del anticuerpo monoclonal anti EhCP5r como método inmunodiagnóstico. Conclusiones. Se logro la producción de un anticuerpo monoclonal específico contra EhCP5r que permite diferenciar Entamoeba histolytica de Entamoeba dispar.

scholarly journals In vitro study of antiamoebic activity of methanol extract of fruit of Pimpinella anisum on trophozoites of Entamoeba histolytica HM1-IMSS

2013 ◽  
Vol 12 (16) ◽  
pp. 2065-2068
Author(s):  
Y Quintilde ones Gutieacute rrez ◽  
M J Verde Star ◽  
C Rivas Morales ◽  
Or ◽  
A ay Caacute rdenas ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Methanol Extract ◽  
In Vitro Study ◽  
Vitro Study ◽  
Pimpinella Anisum ◽  
Antiamoebic Activity

In vivo and in vitro Antiasthmatic Studies of Plant Piper longum Linn.

2012 ◽  
Vol 8 (3) ◽  
pp. 192-197 ◽  
Author(s):  
Dhirender Kaushik ◽  
Ruby Rani ◽  
Pawan Kaushik ◽  
Disha Sacher ◽  
Jyoti Yadav
Keyword(s):  
Piper Longum

The Action of Ipecacuanha Alkaloids on Entamoeba histolytica and some other Entozoic Amoebae in Culture

Parasitology ◽  
1926 ◽  
Vol 18 (2) ◽  
pp. 206-223 ◽  
Author(s):  
Clifford Dobell ◽  
P. P. Laidlaw
Keyword(s):  
Experimental Evidence ◽  
Entamoeba Histolytica ◽  
Therapeutic Agent ◽  
Human Beings ◽  
High Dilution ◽  
Amoebic Dysentery ◽  
Complete Ignorance

It is now recognized, by all competent observers, that emetine is a specific therapeutic agent for the treatment of amoebic dysentery; but although it is known that a thorough course of emetine, in some form, will eradicate an infection with Entamoeba histolytica from most human beings, there is still complete ignorance as to how the cure is effected. The obvious hypothesis, that emetine is a specific poison for E. histolytica, has not hitherto been supported by adequate experimental evidence. It is true that observers have described experiments in which the alkaloid, in high dilution, has apparently killed various amoebae in vitro; but these experiments either ignored the peculiarly specific action of emetine in vivo on E. histolytica, or were carried out without adequate controls.

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