Puva can suppress the expression of cell adhesion molecules and surface antigens of peripheral blood mononuclear cells

Objective To examine the gene expression pattern of Peripheral Blood Mononuclear Cells （PBMCs）and to explore exercise-sensitive genes correlative to bone metabolism in PBMCs. Thus to provide a theoretical basis for exercise intervention to prevent and treat osteoporosis. Methods Uphill (+8° ) and downhill (-8° ) training were used for the exercise loading in two-month-old male Sprague-Dawley rats. The exercise method performed at 25 m/min, training 50 min/d, 5 d/wk, for 12 wk, respectively. Bone mineral density of distal femurs was measured using dual X-ray absorptiometry, and the expressed gene profile of PBMCs was examined using Gene Chip IVT Labeling Kit (Affymetrix). Results Compared with control (CON) group, the BMD of the femur in the downhill (DOWN) group was significantly increased. Compared with the uphill （UP）group, the BMD and BMC of the femur in the DOWN group were significantly increased. There were 38 genes detected differentially expressed between two exercise groups together with CON group. The expression of genes modified by running involved in immunity, cell proliferation，Rheumatoid arthritis，Cell adhesion molecules and Tnf signaling pathway. There were 105 differently expressed genes between the DOWN group and the UP group which were mainly enriched in biological processes and pathways such as response to hydrogen peroxide，lipopolysaccharide，cell factor and mechanical stimulus，Cell adhesion molecules，cell migration，collagen biosynthetic process and Tnf signaling pathway. Tnf, Cxcl2, Ccl2, Jun and Mmp9 as the key nodes of protein interaction network were identified as candidate genes related to bone metabolism and sensitive to exercise. Conclusions With weight gaining，age increasing and training time prolonging, long-term and high-intensity exercise will be harm for bone. At the same time and same running speed, downhill running conduces to increase bone density more than uphill running. It may be associated with differential expression of exercise-sensitive genes involved in bone metabolism in PBMCs.

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Altered adhesion molecules expression on peripheral blood mononuclear cells from patients with systemic sclerosis and clinical correlations

Clinical Rheumatology ◽

10.1007/s10067-009-1124-0 ◽

2009 ◽

Vol 28 (7) ◽

pp. 847-851 ◽

Cited By ~ 4

Author(s):

Heloisa Helena Braga Sawaya ◽

Romy Beatriz Christmann de Souza ◽

Solange Carrasco ◽

Claudia Goldenstein-Schainberg

Keyword(s):

Systemic Sclerosis ◽

Adhesion Molecules ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Clinical Correlations

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Examination of Peripheral Blood Mononuclear Cells and Sera from Thai Adults Naturally Infected with Malaria in Assays of Blastogenic Responsiveness to Mitogenic Lectins and Allogeneic Cell Surface Antigens

Infection and Immunity ◽

10.1128/iai.30.3.781-785.1980 ◽

1980 ◽

Vol 30 (3) ◽

pp. 781-785

Author(s):

Richard P. MacDermott ◽

Robert A. Wells ◽

Sandor Zolyomi ◽

Kathchrinnee Pavanand ◽

Pirom Phisphumvidhi ◽

...

Keyword(s):

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Surface Antigens ◽

Pokeweed Mitogen ◽

Mixed Leukocyte Reaction ◽

Cell Surface Antigens ◽

Peripheral Blood Mononuclear ◽

Allogeneic Cell ◽

Blood Mononuclear Cells

We have previously observed that Thai adults who are infected with malaria have a loss of peripheral blood T cells, and that patient sera contain lymphocytotoxic antibodies. In the present study, we examined peripheral blood mononuclear cells from Thai adults naturally infected with Plasmodium falciparum and Plasmodium vivax for the capacity to undergo blastogenesis in response to phytohemagglutinin, concanavalin A, pokeweed mitogen, and allogeneic cell surface antigens in a one-way mixed leukocyte reaction. In addition, sera from actively infected patients were examined with regard to suppressive capabilities toward normal lymphocyte blastogenesis by using the same assays. We found that patient mononuclear cells exhibited normal reactivity to phytohemagglutinin, concanavalin A, and pokeweed mitogen when compared with controls. However, peripheral blood mononuclear cells from patients had a decreased stimulatory capacity in the allogeneic mixed leukocyte reaction, and P. vivax , but not P. falciparum , lymphocytes exhibited decreased responsiveness in the mixed leukocyte reaction. Furthermore, sera from patients with active malaria induced decreased responsiveness by normal mononuclear cells to phytohemagglutinin and concanavalin A, but not pokeweed mitogen; pooled P. falciparum sera caused decreased responsiveness to allogeneic cell surface antigens in the mixed leukocyte reaction. These studies indicate that despite the lost of circulating T cells during the course of infection with malaria, blastogenic responsiveness remains intact, and that sera from patients with malaria are capable of exerting negative immunoregulatory effects.

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