In vivo 2D 1H NMR of mdx mouse muscle and myoblast cells during fusion: Evidence for a characteristic signal of long chain fatty acids

1993 ◽  
Vol 3 (5-6) ◽  
pp. 433-438 ◽  
Author(s):  
B. Gillet ◽  
B.-T. Doan ◽  
C. Verre-Serrie ◽  
B. Barbere ◽  
G. Berenger ◽  
...  
Keyword(s):  
Fatty Acids ◽  
1H Nmr ◽  
Mdx Mouse ◽  
Long Chain Fatty Acids ◽  
Mouse Muscle ◽  
Characteristic Signal ◽  
Myoblast Cells ◽  
Long Chain ◽  
Chain Fatty Acids

The effect of a blend of dietary unesterified and saturated long-chain fatty acids on the performance of dairy cows in mid-lactation

1990 ◽  
Vol 41 (1) ◽  
pp. 129 ◽  
Author(s):  
KR King ◽  
CR Stockdale ◽  
TE Trigg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Milk Fat ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acids ◽  
Long Chain Fatty Acid ◽  
Long Chain ◽  
Chain Fatty Acids

This experiment studied the effects of feeding a supplement of a blend of unesterified and saturated long-chain fatty acids on the productivity of dairy cows in mid-lactation. Twenty-three cows in their fourth month of lactation were individually fed ad libitum, a mixed balanced ration based on maize silage, lucerne hay and rolled grain. Varying quantities, up to 1020 g cow-1 day-1 of the fatty acid supplement, were mixed into the ration. Yields of milk and milk products were linearly related to total long-chain fatty acid intake. Milk fat content increased linearly while milk protein content averaged 3.59 (s.d. � 0.15)%. The marginal returns from feeding 1 kg of the supplement were 3.3 kg milk, 0.33 kg fat and 0.07 kg protein. The proportions of C 10:0, C12:0 and C 14:0 fatty acids in milk were decreased, while those of C 18:0 and C18:1 were increased as the result of feeding long-chain fatty acids. The concentration of lipid in plasma was increased, but acetate and D-(3)-hydroxybutyrate levels in blood remained unchanged with increased levels of dietary long-chain fatty acid. Efficiency of milk production was increased by 11% from feeding 1 kg of the supplement. In vivo digestibilities of dry matter, neutral and acid detergent fibres, and dietary long-chain fatty acids were unaffected by supplement.

scholarly journals Long chain fatty acids: in vitro cholecystokinin production and in vivo energy intake and body composition alteration

2011 ◽  
Vol 70 (OCE3) ◽  
Author(s):  
C. J. Harden ◽  
A. N. Jones ◽  
T. Maya-Jimenez ◽  
M. E. Barker ◽  
N. J. Hepburn ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Composition ◽  
Energy Intake ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

Long-chain fatty acids differentially alter lipogenesis in bovine and caprine mammary slices

2012 ◽  
Vol 80 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Laurence Bernard ◽  
Mohamad B. Montazer Torbati ◽  
Benoit Graulet ◽  
Christine Leroux ◽  
Yves Chilliard
Keyword(s):  
Fatty Acids ◽  
Mammary Tissue ◽  
Mrna Levels ◽  
Long Chain Fatty Acids ◽  
Expression Of Genes ◽  
Cultured Slices ◽  
Long Chain ◽  
Chain Fatty Acids ◽  
Mammary Tissues

Indirect comparisons from studies in vivo have suggested that caprine mammary tissue is less sensitive than bovine mammary tissue to the anti-lipogenic effect of long-chain fatty acids (LCFA), including specific rumen biohydrogenation (RBH) intermediates of polyunsaturated fatty acids (PUFA). Our objective was to investigate the effects on lipogenesis of 18-carbon LCFA differing in the degree of unsaturation and/or double bond conformation using cultured slices of bovine and caprine mammary tissues. Mammary tissues were collected from five multiparous Holstein × Normande cows and six multiparous Alpine goats in mid lactation. The expression of genes involved in milk component synthesis was measured in tissues collected at slaughter and after slice preparation:FASN, SCD1, CD36, SREBF1andPPARG1mRNA levels were higher in bovine than caprine samples, whereas the opposite was observed forCSN2mRNA levels. Bovine and caprine mammary slices were incubated for 20 h in a medium with BSA (control), cis-9-18 : 1, 18 : 2n-6, 18 : 3n-3, cis-9, trans-11-CLA, or trans-10, cis-12-CLA (the latter at 3 increasing concentrations: C1 (0·11 mm), C2 (0·16 mm), C3 (0·37 mm)). Lipogenesis was estimated by measuring the incorporation of14C-acetate into total lipid. Significant differences of individual LCFA (P < 0·05) were observed between species: bovine tissue showed a decrease in total lipogenesis with 18 : 2n-6, 18 : 3n-3, trans-10,cis-12-CLA (C2 and C3) while caprine tissue showed an increase after treatment with 18 : 3n-3, cis-9, trans-11-CLA or trans-10, cis-12-CLA (C3). These results were not related to the mRNA abundance of our set of genes in the mammary slices after incubation. In conclusion, this study demonstrates that caprine mammary slices reacted differently from bovine mammary slices to the anti-lipogenic activity of specific LCFA and suggests that regulation of lipogenesis via other genes and/or at protein level and enzyme activity may be involved.

scholarly journals Ruminal Fermentation In Vivo as Influenced by Long-Chain Fatty Acids

1986 ◽  
Vol 69 (5) ◽  
pp. 1293-1301 ◽  
Author(s):  
William Chalupa ◽  
Bonnie Vecchiarelli ◽  
Andrew E. Elser ◽  
D.S. Kronfeld ◽  
David Sklan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Long Chain Fatty Acids ◽  
Ruminal Fermentation ◽  
Long Chain ◽  
Chain Fatty Acids
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