Comparative study of skin testing with PPD and new tuberculins by the WHO Mantoux test

An improved multiple puncture technique (MPT) for tuberculin testing is applied with a nine-pointed plastic ring covered by a tube containing a specially concentrated liquid tuberculin. A high degree of positive and negative agreement was obtained with this test when compared to the Mantoux test (PPD, 5 TU). The reactions from the multiple puncture technique are discrete, single, and circular, and they are easy to read and measure. Erythema and induration from this test are smaller than from the Mantoux test (PPD, 5 TU). Children appear to show less fear and felt less pain with the multiple point test than with the Mantoux test. "Wet" tuberculin on multiple points eliminates two possible variables associated with "dry" tuberculin on the points–the length of time that the tines are held in the skin and the moisture content of the skin.

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Asthma in children. VIII. A comparative study of the direct and indirect (local passive transfer) methods of skin testing with the scratch and intradermal technics

Journal of Allergy ◽

10.1016/s0021-8707(29)90105-2 ◽

1929 ◽

Vol 1 (1) ◽

pp. 99-100

Keyword(s):

Comparative Study ◽

Skin Testing ◽

Passive Transfer ◽

Asthma In Children

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Comparative sensitivity of the test with tuberculosis recombinant allergen, containing ESAT6-CFP10 protein, and Mantoux test with 2 TU PPD-L in newly diagnosed tuberculosis children and adolescents in Moscow

10.1101/343665 ◽

2018 ◽

Author(s):

Liudmila Slogotskaya ◽

Elena Bogorodskaya ◽

Diana Ivanova ◽

Tatiana Sevostyanova

Keyword(s):

Children And Adolescents ◽

Mass Screening ◽

Skin Testing ◽

High Sensitivity ◽

High Specificity ◽

Cost Effective ◽

Mantoux Test ◽

Recombinant Allergen ◽

Newly Diagnosed ◽

Comparative Sensitivity

AbstractBackground. A group of Russian scientists has developed Diaskintest, which comprises Mycobacterium tuberculosis-specific recombinant proteins CFP10-ESAT6, for skin testing (0.2 µg/0.1 ml).Study purpose: to evaluate the comparative sensitivity of TST with 2 TU PPD-L and a skin test with tuberculous recombinant allergen (Diaskintest) containing the ESAT6-CFP10 protein in children and adolescents with newly diagnosed respiratory tuberculosis during mass screening in the primary medical service in Moscow, 2013–2016.Materials and methods. The trial was a comprehensive retrospective group study of children and adolescents diagnosed in Moscow with respiratory tuberculosis in 2013–2016, aged 0 to 17 years inclusive. From 441 patients selected for analysis 408 patients had both tests (TST with 2 TU PPD-L and Diaskintest) performed, in 193 patients both tests were given simultaneously, of them 162 patients were BCG-vaccinated.Results. Comparative results of both tests in 408 patients with tuberculosis: at cut-off 5 mm, both tests has similar sensitivity: Diaskintest 98.3 % (95 % CI 97.0–99.6 %), TST 98.0 % (95 % CI 96.7–99.4 %), at cut-off 10 mm, the sensitivity decreases for both tests: Diaskintest 90.0 % (95 % CI 87.0–93.0 %), TST 88.7 % (95 % CI 85.6–91.9 %), but at cut-off 15 mm, the decrease in sensitivity is statistically significant: for Diaskintest 61.5 % (95 % CI 56.7–66.3 %), and for TST 46.3 % (95 % CI 41.4–51.3 %), p <0.0001.The results of simultaneous setting of tests on different hands in 193 people (including 162 BCG-vaccinated), do not differ from the results for 408 people.The correlation between the results of Diaskintest and TST was significant in all groups.Conclusion. In children and adolescents with respiratory tuberculosis, Diaskintest of 0.2 µg/ml and the Mantoux test with 2 TU PPD-L have high sensitivity (98%) at a cut-off of 5 mm; however, at cut-off 15 mm sensitivity is significantly reduced, and the decrease is more pronounced in the Mantoux test. The advantage of Diaskintest is that, unlike the Mantoux test, it has high specificity under the conditions of mass BCG vaccination. The test is cost-effective, simple to carry out, and can be used in mass screening.

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COMPARATIVE STUDY OF SKIN TESTING WITH EXTRACTS OF MITE ALLERGENS FROM DIFFERENT MANUFACTURERS FOR SPECIFIC ALLERGY DIAGNOSTICS IN PATIENTS WITH RESPIRATORY ALLERGIC DISEASES

Asthma and allergy ◽

10.31655/2307-3373-2020-1-14-21 ◽

2020 ◽

pp. 14-21

Author(s):

A. Ye. Bogomolov ◽

S. V. Zaikov ◽

B. A. Stremedlovsky

Keyword(s):

Comparative Study ◽

Skin Testing ◽

Allergic Diseases ◽

Allergy Diagnostics

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Inhalant allergens in respiratory disorders: A comparative study on the intradermal skin testing, immunoglobulin E levels, and spirometry in South Indian population

Indian Journal of Immunology and Respiratory Medicine ◽

10.18231/2456-012x.2017.0006 ◽

2017 ◽

Vol 2 (2) ◽

pp. 45-49

Keyword(s):

Comparative Study ◽

Indian Population ◽

Immunoglobulin E ◽

Skin Testing ◽

South Indian Population ◽

Respiratory Disorders ◽

South Indian ◽

Inhalant Allergens

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A Comparative Study of Tuberculin Reactions to 2 TU and 5 TU of Purified Protein Derivative in a Tertiary Care Center

Journal of Medical Sciences and Health ◽

10.46347/jmsh.2021.v07i01.015 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Chinthana B S ◽

Beere Gowda Y C ◽

Narendra R R ◽

Shivaraja A ◽

Prasad K N V

Keyword(s):

Skin Testing ◽

Care Center ◽

Tertiary Care ◽

Tween 80 ◽

Mantoux Test ◽

Tuberculin Skin Testing ◽

Current Recommendation ◽

Purified Protein Derivative ◽

Institutional Ethics ◽

The Mean

Introduction: Globally, tuberculosis (TB) is the second leading cause of deaths related to an infectious disease, after HIV. Tuberculin skin testing (TST) which is also called Mantoux test or purified protein derivative (PPD) test remains a useful tool to diagnose TB in children. Current recommendation is to use 2 TU PPD RT23 for all diagnostic purposes. TST or Mantoux test is considered positive if the induration is 10 mm or more. Due to non-availability of 2 TU, unstandardized TST with 5, 10 TU RT23 is in practice which can cause problems in reading of test results. Objectives: The objectives of the study were to compare the tuberculin reaction sizes to 2 TU and 5 TU doses of RT 23 with Tween 80 at 46–48 h and 76–78 h. Methodology: All children who were aged < 18 years and are known case of TB were included in the study. Each child was administered dual intradermal injections with 2 TU and 5 TU doses of tuberculin on the volar aspect of the forearm until a wheal was visible at the injected area. The reactions to both the tests were read at 46–48 h and 70–72 h. Institutional Ethics Committee approval was taken before start of the study. The statistical analysis was performed using SPSS 22 version software. Results A total number of cases included in the study during the study period were 81 cases, out of which 52 (64.19%) were male and 29 (35.8%) were female. Among those 81 cases, the mean reaction to 2 TU at 46–48 h was 12.79 mm and at 76–78 h was 15.33 mm. The mean reaction to 5 TU at 46–48 h was 18.89 mm and at 76–78 h was 22.80 mm. Among the cases, the mean size of induration at 46–48 h was lesser than that of 76–78 h in both 2 TU and 5 TU and it was statistically significant. Reactions to 2 TU and 5 TU PPD can be made comparable using the equation, 5 TU = (2 TU × 0.599) + 11.233 at 46–48 h and 5 TU = (2 TU × 0.074) + 21.66 at 76–78 h. ConclusionThus to conclude, cutaneous hypersensitivity to 2 TU PPD can be made comparison using the equation, 5 TU = (2 TU × 0.

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