Asthma in children. VIII. A comparative study of the direct and indirect (local passive transfer) methods of skin testing with the scratch and intradermal technics

Passive transfer of tritiated thymidine-labeled lymphoid cells sensitized to the simple chemical DNFB into homologous guinea pigs resulted in positive contact skin reactions 24 hours after skin testing with DNFB. Labeled sensitized cells were found to accumulate at these sites, whereas, labeled nonsensitized lymphoid cells did not appear non-specifically in contact skin reaction sites. The labeled cells were small and large lymphocytes and immature cells of the lymphoid series. The maximum reactions were obtained at 24 hours, with an average of 3.4 per cent of the infiltrating mononuclear cells showing a label. At 48 hours, the macro- and microscopic reactions were similar to the 24 hour reactions but diminished in intensity, and the number of labeled cells in the infiltrates had decreased to 1 per cent of the total infiltrating mononuclear cells. ¼ to ⅓ of the labeled cells were found within the epidermis in the test skin sites. These data have indicated that contact sensitivity, like tuberculin sensitivity, required the sensitized cell to initiate the skin reaction and that the majority of the cellular infiltrate was the result of non-specific host response to injury.

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Inhalant allergens in respiratory disorders: A comparative study on the intradermal skin testing, immunoglobulin E levels, and spirometry in South Indian population

Indian Journal of Immunology and Respiratory Medicine ◽

10.18231/2456-012x.2017.0006 ◽

2017 ◽

Vol 2 (2) ◽

pp. 45-49

Keyword(s):

Comparative Study ◽

Indian Population ◽

Immunoglobulin E ◽

Skin Testing ◽

South Indian Population ◽

Respiratory Disorders ◽

South Indian ◽

Inhalant Allergens

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Exercise-Induced Asthma in Children: a Comparative Study of Free and Treadmill Running

Annals of Allergy Asthma & Immunology ◽

10.1016/s1081-1206(10)62963-8 ◽

1998 ◽

Vol 80 (3) ◽

pp. 232-236 ◽

Cited By ~ 14

Author(s):

Servando Garcia de la Rubia ◽

Manuel J Pajaron-Fernandez ◽

Manuel Sanchez-Solis ◽

Ignacio Martinez-Gonzalez Moro ◽

Domingo Perez-Flores ◽

...

Keyword(s):

Comparative Study ◽

Treadmill Running ◽

Asthma In Children ◽

Exercise Induced

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A comparative study on the effect of nebulized budesonide and levosalbutamol versus ipratropium bromide and levosalbutamol in the management of acute asthma in children aged 5-11 years

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20205507 ◽

2020 ◽

Vol 8 (1) ◽

pp. 65

Author(s):

Jose P. Cyril ◽

Baburaj S. ◽

Priya S. Nair ◽

Tinu A. Kuruvilla ◽

Bobby C. ◽

...

Keyword(s):

Comparative Study ◽

Ipratropium Bromide ◽

Study Groups ◽

Diagnostic Methods ◽

Maximum Effect ◽

Treatment Groups ◽

Chronic Inflammatory Condition ◽

The Mean ◽

Asthma In Children ◽

Group B

Background: Asthma is a non-communicable chronic inflammatory condition of lung airways. The availability of new diagnostic methods, introduction of a number of drugs, both oral and inhaled has revolutionized management of asthma in children. Goal was to achieve maximum effect with least amount of medication and allowing infrequent use of quick relievers. The present study was, therefore, designed to compare the effectiveness of single dose nebulization with combination of nebulized budesonide and levosalbutamol (group A) versus commonly used ipratropium bromide and levosalbutamol (group B) in children (5-11 years) with mild-moderate exacerbation of asthma.Methods: Was an observational comparative study involving 2 treatment groups of children in age group of (5-11) years, with mild-moderate exacerbation of asthma assessed by peak expiratory flow rate (PEFR) and pulmonary score (PS).Results: Of 160 children analyzed, post nebulization mean predicted PEFR improved in both of the study groups, and the mean PS decreased in both the groups post nebulization which was statistically significant (p<0.001). But when comparing between the groups, the mean percentage of improvement in predicted PEFR and PS were almost similar.Conclusions: Even though, both the groups gave the same end result the group in which budesonide was used had a higher recovery time with the least number of nebulization.

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BONE MARROW AS SOURCE OF CELLS IN REACTIONS OF CELLULAR HYPERSENSITIVITY

Journal of Experimental Medicine ◽

10.1084/jem.128.6.1425 ◽

1968 ◽

Vol 128 (6) ◽

pp. 1425-1435 ◽

Cited By ~ 53

Author(s):

David M. Lubaroff ◽

Byron H. Waksman

Keyword(s):

Bone Marrow ◽

Lymph Node ◽

Bone Marrow Cells ◽

Skin Testing ◽

Skin Lesions ◽

Passive Transfer ◽

Normal Bone ◽

Lymph Node Cells ◽

High Doses ◽

Marrow Cells

Splenectomy or thymectomy of adult Lewis rats following sensitization with tubercle bacilli did not affect their ability to develop delayed skin lesions upon skin testing with PPD. The presence of the thymus also had no significant effect on reactivity of the recipients in passive transfer experiments. The passive transfer of tuberculin hypersensitivity with sensitized lymph node cells to thymectomized, irradiated recipients depended on the simultaneous or prior injection of normal bone marrow cells. When lymph node cell transfer was performed shortly after irradiation and injection of marrow, high doses of marrow cells (3.5–4.0 x 108 were required to permit eliciting reactions of reasonable intensity. If, however, periods of 7–10 days elapsed between the injection of bone marrow and sensitized lymph node cells, lower doses of marrow were sufficient for comparable reactions. Normal thymus, spleen, lymph node, or peritoneal exudate cells, even at high doses could not be substituted for the bone marrow in producing good tuberculin reactions.

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