THE ACTIONS OF FOLLICULAR FLUID FACTORS ON STEROIDOGENESIS BY CULTURED OVARIAN GRANULOSA CELLS

1983 ◽  
pp. 127-131
Author(s):  
F. LEDWITZ-RIGBY ◽  
B.W. RIGBY
Keyword(s):  
Granulosa Cells ◽  
Follicular Fluid ◽  
Ovarian Granulosa Cells

scholarly journals LRH-1 High Expression in the Ovarian Granulosa Cells of PCOS Patients

2021 ◽  
Author(s):  
Xiao Yang ◽  
Qiumin Wang ◽  
Ying Wang ◽  
Tian Song ◽  
Yanjun Zheng ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Pearson Correlation ◽  
Statistical Significance ◽  
Control Group ◽  
Vitro Fertilization ◽  
Real Time Quantitative Pcr ◽  
Ovarian Granulosa Cells

Abstract Purpose: Polycystic ovary syndrome (PCOS) is considered as one of the most common endocrine disorder with heterogeneity. There are also reports that liver receptor homolog 1 [LRH-1 or nuclear receptor subfamily 5 group A member 2 (NR5A2)] plays an important role in the reproductive system. But up to now, there are no reports related to the link with PCOS and LRH-1. In this study, we aimed to detect the LRH-1 expression in the ovarian granulosa cell of PCOS patients and explore the potential relationship between LRH-1 and PCOS. Methods: 146 follicular fluid sample were collected in this study, including 72 from PCOS patients and 74 from control patients who underwent intracytoplasmic sperm injection (ICSI) or in vitro fertilization-embryo transfer (IVF-ET). The ovarian granulosa cells were extracted from the patient's follicular fluid by magnetic-activated cell sorting (MACS) method, and the real-time quantitative PCR (qRT-PCR) was used to measure the expression of LRH-1 in ovarian granulosa cells. Then we analyzed the correlation between the expression level of LRH-1 and the clinical characteristics of patient by using Pearson Correlation analysis. Results: The expression of LRH-1 was significantly higher in PCOS patients ovarian granulosa cells than that in the control patients [vs(1.38±0.47)vs(1.03±0.32), t=5.327, p<0.0001], and it was positively correlated with antral follicles counting (AFC) (r=0.3607, p<0.0001)and the serum AMH(r=0.2662, p=0.0012)\LH(r=0.2518, p=0.0022)\T(r=0.2516, p=0.0022) in all patients. No statistical significance between LRH-1 and BMI, FSH, HOMA-IR, DHE-S, progesterone. Conclusions: Compared with the control group, we found that LRH-1 was highly expressed in the ovarian granulosa cells of PCOS patients. Our study has revealed the relationship between the LRH-1 expression and PCOS, which suggested that LRH-1 may play an important role in ovulation disorders. While this finding provided new ideas for the study of the pathogenesis, it also provided a theoretical basis for the clinical diagnosis and treatment for PCOS.

scholarly journals Involvement of Serum Amyloid A1 in the Pathogenesis of Insulin Resistance in Ovarian Granulosa Cells in Polycystic Ovary Syndrome

2021 ◽  
Author(s):  
Qinling Zhu ◽  
Yue Yao ◽  
Lizhen Xu ◽  
Hasiximuke Wu ◽  
Wangsheng Wang ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Glucose Transporter ◽  
Polycystic Ovary ◽  
Serum Amyloid ◽  
Peripheral Tissues ◽  
Akt Phosphorylation ◽  
Ovarian Granulosa Cells ◽  
Serum Amyloid A1

Abstract Background: Insulin resistance (IR) contributes to ovarian dysfunctions in PCOS patients. Serum amyloid A1 (SAA1) is an acute phase protein produced primarily by the liver in response to inflammation. In addition to its role in inflammation, SAA1 may participate in IR development in peripheral tissues. Yet, expressional regulation of SAA1 in the ovary and its role in the pathogenesis of ovarian IR in PCOS remain elusive. Methods: Follicular fluid and granulosa cells were collected from PCOS and non-PCOS patients with and without IR to measure SAA1 abundance for analysis of its correlation with IR status. The effects of SAA1 on its own expression and insulin signaling pathway were investigated in cultured primary granulosa cells. Results: Ovarian granulosa cells were capable of producing SAA1, which could be induced by SAA1 per se. Moreover, the abundance of SAA1 significantly increased in granulosa cells and follicular fluid in PCOS patients with IR. SAA1 treatment significantly attenuated insulin-stimulated membrane translocation of glucose transporter 4 and glucose uptake in granulosa cells through induction of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression with subsequent inhibition of Akt phosphorylation. These effects of SAA1 could be blocked by inhibitors for toll-like receptors 2/4 (TLR 2/4) and nuclear factor kappa light chain enhancer of activated B (NF-κB). Conclusions: Human granulosa cells are capable of feedforward production of SAA1, which significantly increased in PCOS patients with IR. Excessive SAA1 reduces insulin sensitivity in granulosa cells via induction of PTEN and subsequent inhibition of Akt phosphorylation upon activation of TLR2/4 and NF-κB pathway. These findings highlight that local excessive synthesis of SAA1 is associated with IR development in granulosa cells of PCOS patients.

scholarly journals Effects of Multicycle Gonadotropin-releasing Hormone Antagonist Protocols on Oxidative Stress of Follicular Fluid and Ovarian Granulosa Cells

2020 ◽  
Author(s):  
Yucong Ma ◽  
Zhiming Zhao ◽  
Guimin Hao ◽  
Na Cui ◽  
Yanli Fan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Gonadotropin Releasing Hormone ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Releasing Hormone ◽  
Ovarian Granulosa Cells ◽  
Cycle Group

Abstract Background: Repeated controlled ovarian stimulation (COS) has adverse effects on clinical pregnancy outcomes in in vitro fertilization-embryo transfer (IVF-ET) patients. The effect of repeated antagonist protocols on oxidative stress (OS) in follicular fluid (FF) and ovarian granulosa cells (GC) remains unclear. Objective: To study the effects of repeated multicycle gonadotropin-releasing hormone antagonist (GnRH-ant) protocols on OS markers of FF and ovarian GC. Methods: A total of 145 patients were enrolled and divided into four groups: 1 cycle group (n = 42), 2 cycles group (n = 37), 3 cycles group (n = 45), and 4-5 cycles group (n = 21). The FF and ovarian GC of the patients were collected on the day of last egg retrieval. Levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in FF and ovarian GC were analyzed. Results: With the increase of GnRH-ant protocol cycles, the serum estradiol levels on human chorionic gonadotrophin (hCG) injection day, number of retrieved oocytes, 2PN embryos, and the rates of high-quality embryos, implantation and clinical pregnancy were all significantly decreased, while the gonadotropin (Gn) usage showed an increasing trend. Compared with 1 or 2 cycles, the 8-OHdG and SOD were significantly increased in the 3 to 5 cycles, while the CAT and GSH-Px levels were significantly decreased. Conclusion: Repeated COS with the use of GnRH-ant protocols results in OS and changes the follicle microenvironment of FF and GC, possibly leading to poor IVF outcomes in patients with 3-5 cycles of COS.

scholarly journals Mouse ovarian granulosa cells produce urokinase-type plasminogen activator, whereas the corresponding rat cells produce tissue-type plasminogen activator.

1987 ◽  
Vol 105 (2) ◽  
pp. 977-981 ◽  
Author(s):  
R Canipari ◽  
M L O'Connell ◽  
G Meyer ◽  
S Strickland
Keyword(s):  
Plasminogen Activator ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Messenger Rna ◽  
Tissue Type ◽  
Ovarian Granulosa Cells ◽  
Type Plasminogen Activator ◽  
Mouse Cells ◽  
The Difference

It is well established that rat ovarian granulosa cells produce tissue plasminogen activator (tPA). The synthesis and secretion of the enzyme are induced by gonadotropins, and correlate well with the time of follicular rupture in vivo. We have found that in contrast, mouse granulosa cells produce a different form of plasminogen activator, the urokinase-type (uPA). As with tPA synthesis in the rat, uPA production by mouse granulosa cells is induced by gonadotropins, dibutyryl cAMP, and prostaglandin E2. However, dexamethasone, a drug which has no effect on tPA synthesis in rat cells inhibits uPA synthesis in the mouse. Results of these determinations made in cell culture were corroborated by examining follicular fluid, which is secreted in vivo predominantly by granulosa cells, from stimulated rat and mouse ovarian follicles. Rat follicular fluid contained only tPA, and mouse follicular fluid only uPA, indicating that in vivo, granulosa cells from the two species are secreting different enzymes. The difference in the type of plasminogen activator produced by the rat and mouse granulosa cells was confirmed at the messenger RNA level. After hormone stimulation, only tPA mRNA was present in rat cells, whereas only uPA mRNA was found in mouse cells. Furthermore, the regulation of uPA levels in mouse cells occurs via transient modulation of steady-state levels of mRNA, a pattern similar to that seen with tPA in rat cells.

Sign in / Sign up

Export Citation Format

Share Document