Determination of Thymosin β4 In Cultured Cell Lines

1985 ◽  
pp. 1093-1096 ◽  
Author(s):  
E. HANNAPPEL ◽  
W. LEIBOLD
Keyword(s):  
Cell Lines ◽  
Thymosin Β4 ◽  
Cultured Cell

Phytochemical Constituents of Annona reticulata and their Cytotoxic Activity

2020 ◽  
Vol 17 (3) ◽  
pp. 206-210
Author(s):  
Ty Viet Pham ◽  
Thang Quoc Le ◽  
Anh Tuan Le ◽  
Hung Quoc Vo ◽  
Duc Viet Ho
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Structural Determination ◽  
Phytochemical Investigation ◽  
Ic50 Values ◽  
Phytochemical Constituents ◽  
First Time ◽  
Annona Reticulata

A phytochemical investigation of the leaves of Annona reticulata led to the isolation and structural determination of β-sitosterol (1), ent-pimara-8(14),15-dien-19-oic acid (2), ent-pimara- 8(14),15-dien-19-ol (3), quercetin (4), quercetin 3-O-α-L-arabinopyranoside (5), and a mixture of quercetin 3-O-β-D-galactopyranoside (6a) and quercetin 3-O-β-D-glucopyranoside (6b). Of these, compounds 2 and 3 were isolated from the genus Annona for the first time. Compound 3 showed strong cytotoxicity against SK-LU-1 and SW626 cell lines with IC50 values of 17.64 ± 1.07 and 19.79 ± 1.41 μg mL-1, respectively.

scholarly journals Apolipoprotein B mRNA editing. Direct determination of the edited base and occurrence in non-apolipoprotein B-producing cell lines.

1990 ◽  
Vol 265 (36) ◽  
pp. 22446-22452 ◽  
Author(s):  
K Boström ◽  
Z Garcia ◽  
K S Poksay ◽  
D F Johnson ◽  
A J Lusis ◽  
...  
Keyword(s):  
Cell Lines ◽  
Apolipoprotein B ◽  
Direct Determination ◽  
Mrna Editing

Lymphocytotoxicity inhibition: a reliable method for HL-A typing of cultured cell lines

1975 ◽  
Vol 14 (3-6) ◽  
pp. 241-246
Author(s):  
W.B. Bias ◽  
D.S. Borgaonkar ◽  
R.S. Kucherlapati ◽  
F.H. Ruddle
Keyword(s):  
Cell Lines ◽  
Reliable Method ◽  
Cultured Cell

scholarly journals An Unprecedented High Content of the Bioactive Flavone Tricin in Huperzia Medicinal Species Used by the Saraguro in Ecuador

2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100
Author(s):  
Chabaco Armijos ◽  
Jorge Ponce ◽  
Jorge Ramírez ◽  
Davide Gozzini ◽  
Paola Vita Finzi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Traditional Medicine ◽  
Cell Lines ◽  
Cancer Cell ◽  
Potent Inhibitor ◽  
Community Living ◽  
Medicinal Species ◽  
Chemopreventive Agent ◽  
Southern Ecuador

The flavone tricin (5,7,4′-trihydroxy-3′,5′-dimethoxyflavone) is considered to be a selective potent inhibitor of different cancer cell lines and a potential colorectal cancer chemopreventive agent. In this paper we describe a reliable UHPLC-UV-ESIMS method for the determination of tricin in Huperzia plants used in the traditional medicine of the Saraguro community living in Southern Ecuador. An unusually high amount of tricin was found in H. brevifolia and H. compacta, which exceeded the content of this flavone determined so far in other plants.

scholarly journals Cultured Cell Sublines Highly Susceptible to Prion Infection

2000 ◽  
Vol 74 (9) ◽  
pp. 4377-4386 ◽  
Author(s):  
Patrick J. Bosque ◽  
Stanley B. Prusiner
Keyword(s):  
Cell Culture ◽  
Infected Cell ◽  
Tumor Cell ◽  
Cell Lines ◽  
Uninfected Cell ◽  
Resistant Cell ◽  
Cultured Cell ◽  
Prion Infection ◽  
Blot Technique ◽  
Prion Propagation

ABSTRACT Cultured cell lines infected with prions produce an abnormal isoform of the prion protein (PrPSc). In order to derive cell lines producing sufficient quantities of PrPSc for most studies, it has been necessary to subclone infected cultures and select the subclones producing the largest amounts of PrPSc. Since postinfection cloning can introduce differences between infected and uninfected cell lines, we sought an approach to generate prion-infected cell lines that would avoid clonal artifacts. Using an improved cell blot technique, which permits sensitive and rapid comparison of PrPSc levels in multiple independent cell cultures, we discovered marked heterogeneity with regard to prion susceptibility in tumor cell sublines. We exploited this heterogeneity to derive sublines which are highly susceptible to prion infection and used these cells to generate prion-infected lines without further subcloning. These infected sublines can be compared to the cognate uninfected cultures without interference from cloning artifacts. We also used susceptible cell lines and our modified cell blot procedure to develop a sensitive and reproducible quantitative cell culture bioassay for prions. We found that the sublines were at least 100-fold more susceptible to strain RML prions than to strain ME7 prions. Comparisons between scrapie-susceptible and -resistant cell lines may reveal factors that modulate prion propagation.

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