5.7 Tissue Engineering and Selection of Cells

The human innate regenerative ability is known to be limited by the intensity of the insult together with the availability of progenitor cells, which may cause certain irreparable damage. It is only recently that the paradigm of tissue engineering found its way to the treatment of irreversibly affected body structures with the challenge of reconstructing the lost part. In the current review, we underline recent trials that target engineering of human craniofacial structures, mainly bone, cartilage, and teeth. We analyze the applied engineering strategies relative to the selection of cell types to lay down a specific targeted tissue, together with their association with an escorting scaffold for a particular engineered site, and discuss their necessity to be sustained by growth factors. Challenges and expectations for facial skeletal engineering are discussed in the context of future treatment.

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Fabrication 3d Tissue Engineering Scaffold Poly(Ethylene) Diacrylate Filled with Aramid Nanofiber: Mechanical Evaluation and Toxicity

International Journal of Innovative Technology and Exploring Engineering - Special Issue ◽

10.35940/ijitee.k2916.1081219 ◽

2019 ◽

Vol 8 (12) ◽

pp. 1997-2006

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

3D Structure ◽

Tissue Engineering Scaffold ◽

Digital Light Processing ◽

Technical Requirements ◽

Preliminary Study ◽

Poly Ethylene ◽

3D Printing Technique ◽

Selection Of

The selection of the optimum scaffold fabrication method becomes challenging due to a variety of manufacturing methods, existing biomaterials and technical requirements. Although, Digital light processing (DLP) 3D printing process is one of the SLA techniques which commonly used to fabricate tissue engineering scaffold, however, there is no report published on the fabrication of tissue engineering scaffold-based PEGDA filled with Aramid Nanofiber (ANFs). Hence, the feasible parameter setting for fabricating this material using DLP technique is currently unknown. This work aims to establish the feasible setting parameter via DLP 3D printing to fabricate PEGDA/ANFs 3D tissue engineering scaffold. Preliminary study has been done to identify the accurate composition and curing time setting in producing scaffold. In this work, the researcher has proved the potential and capability of these novel composition biomaterial PEGDA/ANFs to be print via DLP-3D printing technique to form a 3D structure which is not yet been established and has not reported elsewhere.

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Biomaterials for In Situ Tissue Regeneration: A Review

Biomolecules ◽

10.3390/biom9110750 ◽

2019 ◽

Vol 9 (11) ◽

pp. 750 ◽

Cited By ~ 21

Author(s):

Saba Abdulghani ◽

Geoffrey Mitchell

Keyword(s):

Tissue Engineering ◽

Tissue Regeneration ◽

Cell Function ◽

Predictive Modelling ◽

Ex Situ ◽

Design And Optimization ◽

Injured Region ◽

In Situ Tissue Engineering ◽

Selection Of

This review focuses on a somewhat unexplored strand of regenerative medicine, that is in situ tissue engineering. In this approach manufactured scaffolds are implanted in the injured region for regeneration within the patient. The scaffold is designed to attract cells to the required volume of regeneration to subsequently proliferate, differentiate, and as a consequence develop tissue within the scaffold which in time will degrade leaving just the regenerated tissue. This review highlights the wealth of information available from studies of ex-situ tissue engineering about the selection of materials for scaffolds. It is clear that there are great opportunities for the use of additive manufacturing to prepare complex personalized scaffolds and we speculate that by building on this knowledge and technology, the development of in situ tissue engineering could rapidly increase. Ex-situ tissue engineering is handicapped by the need to develop the tissue in a bioreactor where the conditions, however optimized, may not be optimum for accelerated growth and maintenance of the cell function. We identify that in both methodologies the prospect of tissue regeneration has created much promise but delivered little outside the scope of laboratory-based experiments. We propose that the design of the scaffolds and the materials selected remain at the heart of developments in this field and there is a clear need for predictive modelling which can be used in the design and optimization of materials and scaffolds.

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Lactoferrin Functionalized Biomaterials: Tools for Prevention of Implant-Associated Infections

Antibiotics ◽

10.3390/antibiotics9080522 ◽

2020 ◽

Vol 9 (8) ◽

pp. 522

Author(s):

Emoke Pall ◽

Alexandra Roman

Keyword(s):

Tissue Engineering ◽

Antimicrobial Peptide ◽

Multidrug Resistant ◽

Orthopedic Implant ◽

Bacterial Strains ◽

New Approach ◽

Biomedical Field ◽

Proper Material ◽

Selection Of ◽

Scientific Engineering

Tissue engineering is one of the most important biotechnologies in the biomedical field. It requires the application of the principles of scientific engineering in order to design and build natural or synthetic biomaterials feasible for the maintenance of tissues and organs. Depending on the specific applications, the selection of the proper material remains a significant clinical concern. Implant-associated infection is one of the most severe complications in orthopedic implant surgeries. The treatment of these infections is difficult because the surface of the implant serves not only as a substrate for the formation of the biofilm, but also for the selection of multidrug-resistant bacterial strains. Therefore, a promising new approach for prevention of implant-related infection involves development of new implantable, non-antibiotic-based biomaterials. This review provides a brief overview of antimicrobial peptide-based biomaterials—especially those coated with lactoferrin.

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