Application of MALDI-TOF for Bacterial Identification

2018 ◽  
pp. 101-112 ◽  
Author(s):  
Sachio Tsuchida
Keyword(s):  
Bacterial Identification ◽  
Maldi Tof

scholarly journals Impact of the MALDI-TOF as a tool for bacterial identification in the frequency of isolation of Aerococcus spp and Actinotignum schaalii in urinary tract infection

2019 ◽  
Vol 2 (2) ◽  
pp. 17-21
Author(s):  
Nona Romaní ◽  
Gema Fernández-Rivas ◽  
Sònia Molinos ◽  
Adrián Antuori ◽  
Jun Hao Wang ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Bacterial Identification ◽  
Maldi Tof

scholarly journals Usefulness of the MALDI-TOF MS technology with membrane filter protocol for the rapid identification of microorganisms in perioperative drainage fluids of hepatobiliary pancreatic surgery

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246002
Author(s):  
Kazuyuki Sogawa ◽  
Shigetsugu Takano ◽  
Takayuki Ishige ◽  
Hideyuki Yoshitomi ◽  
Shingo Kagawa ◽  
...  
Keyword(s):  
Membrane Filtration ◽  
High Reliability ◽  
Membrane Filter ◽  
Perioperative Complications ◽  
Bacterial Species ◽  
Bacterial Identification ◽  
Maldi Tof Ms ◽  
Drainage Fluid ◽  
Maldi Tof ◽  
Tof Ms

Surgical site infections (SSIs) are significant and frequent perioperative complications, occurring due to the contamination of the surgical site. The late detection of SSIs, especially organ/space SSIs which are the more difficult to treat, often leads to severe complications. An effective method that can identify bacteria with a high accuracy, leading to the early detection of organ/space SSIs, is needed. Ninety-eight drainage fluid samples obtained from 22 patients with hepatobiliary pancreatic disease were analyzed to identify microorganisms using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) with a new membrane filtration protocol and rapid BACpro® pretreatment compared to sole rapid BACpro® pretreatment. The levels of detail of rapid BACpro® pretreatment with or without filtration were also evaluated for the accuracy of bacterial identification. We found that reliable scores for E. coli and E. faecalis were obtained by inoculation with 1.0 × 104 CFU/ml after preparation of the membrane filter with rapid BACpro®, indicating approximately 10-folds more sensitive compared to sole rapid BACpro® pretreatment in drainage fluid specimens. Among 60 bacterial positive colonies in drainage fluid specimens, the MALDI-TOF MS and the membrane filtration with rapid BACpro® identified 53 isolates (88.3%) with a significantly higher accuracy, compared to 25 isolates in the rapid BACpro® pretreatment group (41.7%) (p < 0.001). Among the 78 strains, 14 enteric Gram-negative bacteria (93.0%) and 55 Gram-positive cocci (87.3%) were correctly identified by the membrane filtration with rapid BACpro® with a high reliability. This novel protocol could identify bacterial species within 30 min, at $2-$3 per sample, thus leading to cost and time savings. MALDI-TOF MS with membrane filter and rapid BACpro® is a quick and reliable method for bacterial identification in drainage fluids. The shortened analysis time will enable earlier selection of suitable antibiotics for treatment of organ/space SSIs to improve patients’ outcomes.

scholarly journals Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS

2015 ◽  
Vol 99 (13) ◽  
pp. 5547-5562 ◽  
Author(s):  
Dominik Ziegler ◽  
Joël F. Pothier ◽  
Julie Ardley ◽  
Romain Kouakou Fossou ◽  
Valentin Pflüger ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Root Nodule ◽  
Bacterial Identification ◽  
Protein Biomarkers ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Routine use of MALDI-TOF MS for anaerobic bacterial identification in clinical microbiology

Anaerobe ◽  
2018 ◽  
Vol 54 ◽  
pp. 191-196 ◽  
Author(s):  
Samantha Shannon ◽  
Daniel Kronemann ◽  
Robin Patel ◽  
Audrey N. Schuetz
Keyword(s):  
Clinical Microbiology ◽  
Bacterial Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Rapid and Accurate Bacterial Identification in Probiotics and Yoghurts by MALDI-TOF Mass Spectrometry

2011 ◽  
Vol 76 (8) ◽  
pp. M568-M572 ◽  
Author(s):  
Emmanouil Angelakis ◽  
Matthieu Million ◽  
Mireille Henry ◽  
Didier Raoult
Keyword(s):  
Mass Spectrometry ◽  
Bacterial Identification ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

2018 ◽  
Vol 15 (8) ◽  
pp. 1695 ◽  
Author(s):  
Pina Caputo ◽  
Maria Di Martino ◽  
Brunella Perfetto ◽  
Francesco Iovino ◽  
Giovanna Donnarumma
Keyword(s):  
Antibiotic Therapy ◽  
Staphylococcus Epidermidis ◽  
Bacterial Identification ◽  
Good Tool ◽  
Accurate Identification ◽  
Ionization Time ◽  
Maldi Tof ◽  
Crystal Violet Assay ◽  
Reference Strains ◽  
Producer Strains

For the management of Staphylococci coagulase-negative infection, often related to biofilm formation, rapid and accurate identification is necessary in choosing a correct antibiotic therapy. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is becoming increasingly important for bacterial identification over traditional methods. Our aim was to validate the use of MALDI to discriminate Staphylococcus epidermidis biofilm-producing strains. Clinical strains coming from suture wires were identified and their protein profiles were compared to that obtained from two ATCC reference strains (biofilm producer and non-producer). MALDI identified the eighteen isolates as S. epidermidis, combining sixteen profiles with the biofilm producer and two with the non-producer, confirming the results of crystal violet assay. Our data highlight that MALDI can be considered a good tool to discriminate between biofilm-producer and non-producer strains of S. epidermidis, thus helping to establish an effective antibiotic therapy.

scholarly journals A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

2019 ◽  
Vol 5 (1) ◽  
pp. 4 ◽  
Author(s):  
Robin Patel
Keyword(s):  
Mass Spectrometry ◽  
Filamentous Fungi ◽  
Bacterial Identification ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Fungal Identification ◽  
Routine Identification ◽  
Tof Ms

As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.

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