Rotaviruses, astroviruses, and sapoviruses as foodborne infections

Fresh vegetables and salads are increasingly implicated in outbreaks of foodborne infections, such as those caused by Listeria monocytogenes, a dangerous pathogen that can attach to the surfaces of the equipment creating robust biofilms withstanding the killing action of disinfectants. In this study, the antimicrobial efficiency of a natural plant terpenoid (thymol) was evaluated against a sessile population of a multi-strain L. monocytogenes cocktail developed on stainless steel surfaces incubated in lettuce broth, under optimized time and temperature conditions (54 h at 30.6 °C) as those were determined following response surface modeling, and in comparison, to that of an industrial disinfectant (benzalkonium chloride). Prior to disinfection, the minimum bactericidal concentrations (MBCs) of each compound were determined against the planktonic cells of each strain. The results revealed the advanced killing potential of thymol, with a concentration of 625 ppm (= 4 × MBC) leading to almost undetectable viable bacteria (more than 4 logs reduction following a 15-min exposure). For the same degree of killing, benzalkonium chloride needed to be used at a concentration of at least 20 times more than its MBC (70 ppm). Discriminative repetitive sequence-based polymerase chain reaction (rep-PCR) also highlighted the strain variability in both biofilm formation and resistance. In sum, thymol was found to present an effective anti-listeria action under environmental conditions mimicking those encountered in the salad industry and deserves to be further explored to improve the safety of fresh produce.

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Frequency of Antimicrobial Resistance in Shiga Toxin-Producing Escherichia coli (STEC) and Non-Typhoidal Salmonella (NTS) Clinical Infections and Association with Epidemiological Factors

Open Forum Infectious Diseases ◽

10.1093/ofid/ofx163.893 ◽

2017 ◽

Vol 4 (suppl_1) ◽

pp. S366-S366

Author(s):

Sanjana Mukherjee ◽

Rebekah Mosci ◽

Chase Anderson ◽

Brian Snyder ◽

James Collins ◽

...

Keyword(s):

Continuous Monitoring ◽

Antibiotic Prescription ◽

Epidemiological Data ◽

Chi Square ◽

Surveillance Network ◽

High Frequencies ◽

Drug Classes ◽

The Us ◽

Prescription Rates ◽

Foodborne Infections

Abstract Background STEC and NTS are leading causes of foodborne infections in the US. Monitoring resistance in these pathogens is essential to understand the distribution of resistance profiles and because of the high likelihood of horizontal transfer of resistance genes to other pathogens. Data involving resistance in clinical STEC and NTS isolates from Michigan is lacking. Methods Clinical STEC (n = 353) and NTS (n = 148) isolates from the MDHHS (2010–2014) were examined for resistance using disk diffusion, E-test or broth microdilution. Case information and epidemiological data for STEC isolates was extracted and associations with resistant infections were determined using chi square tests in SAS 9.3 and EpiInfo™ 7. Results Overall, 31 (8.8%, n = 353) STEC isolates were resistant to at least one antibiotic; high frequencies of resistance were observed for ampicillin (7.4%) and trimethoprim-sulfamethoxazole (4.0%). Resistance to ciprofloxacin (0.28%) and all three drug classes (0.28%) was less common. Preliminary results indicate that O157 resistance to ampicillin (4.8%) and trimethoprim-sulfamethoxazole (3.4%) was higher in Michigan compared with national frequencies (ampicillin = 2.7%, trimethoprim-sulfamethoxazole= 1.5%). Higher resistance frequencies were also observed in counties with high (11.3%) vs. low (7.7%) antibiotic prescription rates. For NTS, 23 (15.5%) isolates were resistant to ≥1 antibiotic. Resistance varied by serotype with high frequencies in Typhimurium (20%, n = 20), Newport (17.6%, n = 17) and Enteritidis (4.8%, n = 42); 11 (7.4%) NTS isolates were resistant to ≥3 antimicrobial classes. Conclusion Continuous monitoring of resistance in clinical STEC and NTS is warranted due to their importance as food pathogens. The identification of risk factors for resistance is crucial to develop alternative prevention practices to reduce the health burden of resistant infections in Michigan, which is not part of the FoodNet surveillance network. Disclosures All authors: No reported disclosures.

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Foodborne infections

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2008/172637 ◽

2008 ◽

Vol 19 (6) ◽

pp. 431-434 ◽

Cited By ~ 1

Keyword(s):

Foodborne Infections

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The Impact of Foodborne Infections and Toxins on International Trade

Microbial Food Contamination ◽

10.1201/9781420039030.sec5 ◽

2000 ◽

Author(s):

Isaac Klinger

Keyword(s):

International Trade ◽

The Impact ◽

Foodborne Infections

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Resistance profile to antimicrobials of Salmonella spp. isolated from human infections

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132004000200005 ◽

2004 ◽

Vol 47 (2) ◽

pp. 193-197 ◽

Cited By ~ 2

Author(s):

Elaine Regina Delicato ◽

Jane Martha Graton Mikcha ◽

Sueli Aparecida Fernandes ◽

Jacinta Sanchez Pelayo

Keyword(s):

Public Health ◽

Antimicrobial Resistance ◽

High Resistance ◽

Salmonella Spp ◽

Resistance Profile ◽

The Public ◽

Human Infections ◽

Foodborne Infections

The purpose of this study was to analyse the profile of antimicrobial resistance among 21 strains of Salmonella isolated from patients with gastroenteritis symptom. It was observed that S. enteritidis was the serotype prevalent. These strains were sensitive to the majority of the antimicrobials tested, however, high resistance was observed in S. typhimurium and S. enterica subsp. enterica serotype 4,5,12:i:-. Surveillance and an efficient monitoring should be priority for the public health for the containment of antimicrobial resistance in foodborne infections.

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Incidence and Susceptibility to Antimicrobial Agents of Listeria spp. and Listeria monocytogenes Isolated from Poultry Carcasses in Porto, Portugal

Journal of Food Protection ◽

10.4315/0362-028x-65.12.1888 ◽

2002 ◽

Vol 65 (12) ◽

pp. 1888-1893 ◽

Cited By ~ 24

Author(s):

PATRÍCIA ANTUNES ◽

CRISTINA RÉU ◽

JOÃO CARLOS SOUSA ◽

NAZARÉ PESTANA ◽

LUÍSA PEIXE

Keyword(s):

Listeria Monocytogenes ◽

Antimicrobial Agents ◽

Polymerase Chain Reaction Method ◽

Poultry Products ◽

High Incidence ◽

Biochemical Identification ◽

Polymerase Chain ◽

The City ◽

Butcher Shops ◽

Foodborne Infections

The occurrence of Listeria spp. and Listeria monocytogenes in 63 samples of Portuguese poultry carcasses obtained from two local butcher shops and one canteen in the city of Porto, Portugal, and the susceptibility of these bacteria to antimicrobial agents allowed for use in human or animal therapeutics were evaluated. All poultry samples were contaminated with Listeria spp., and L. monocytogenes was isolated from 41% (26 of 63) of the samples. Other Listeria species, including L. innocua, L. welshimeri, and L. seeligeri, were also isolated from poultry samples. A multiplex polymerase chain reaction method was used for the identification of all of the Listeria isolates; this method showed total conformity with the conventional method of biochemical identification and proved to be more reliable, faster, and less arduous. In addition, high percentages of Listeria spp. (84%) and L. monocytogenes (73%) isolates were found to be resistant to one or more antimicrobial agents of different groups, and 12 different resistance profiles were recorded. The frequency of the resistance of L. monocytogenes isolates to enrofloxacin and clindamycin is notable. The results of this study suggest a high incidence of L. monocytogenes on Portuguese poultry products available for consumers and indicate that poultry could be a potential vehicle of foodborne infections due to strains of L. monocytogenes that are resistant to antimicrobial agents.

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