LIGHT-PENETRATION CHANGES IN RAT MAMMARY-GLAND TISSUE IN VITRO AFTER OXYTOCIN ADMINISTRATION

1968 ◽  
Vol 42 (4) ◽  
pp. 599-600
Author(s):  
M. LIS ◽  
J. BÍLEK ◽  
I. SKALA ◽  
J. SLABA
Keyword(s):  
Mammary Gland ◽  
Light Penetration ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Rat Mammary Gland

BIOASSAY OF OXYTOCIN BY CHANGES IN LIGHT PENETRATION THROUGH RAT MAMMARY GLAND TISSUE IN VITRO

1971 ◽  
Vol 51 (4) ◽  
pp. 771-NP ◽  
Author(s):  
J. BÍLEK ◽  
I. MIKULÁŠ ◽  
J. SLABA ◽  
I. SKÁLA
Keyword(s):  
Mammary Gland ◽  
Wheatstone Bridge ◽  
Light Penetration ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Rat Mammary Gland

SUMMARY A method for the bioassay of oxytocin in the lactating rat mammary gland in vitro is described. The changes in transparency induced by oxytocin in a flat segment of the gland are recorded by means of a CdS photoresistor and a simple Wheatstone bridge. The sensitivity of the assay in routine use is about 0·5–10 μu. oxytocin, the index of precision being about 0·1.

Spectral Characterization of Fluorescently Labeled Catechol Estrogen 3,4-Quinone-Derived N7 Guanine Adducts and Their Identification in Rat Mammary Gland Tissue

1998 ◽  
Vol 11 (11) ◽  
pp. 1339-1345 ◽  
Author(s):  
Ryszard Jankowiak ◽  
Dan Zamzow ◽  
Douglas E. Stack ◽  
Rosa Todorovic ◽  
Ercole L. Cavalieri ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Spectral Characterization ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Guanine Adducts ◽  
Catechol Estrogen ◽  
Rat Mammary Gland

THE METABOLISM OF TESTOSTERONE BY TISSUE FROM NORMAL AND NEOPLASTIC RAT BREAST

1964 ◽  
Vol 29 (2) ◽  
pp. 103-110 ◽  
Author(s):  
R. J. B. KING ◽  
J. GORDON ◽  
JANET E. HELFENSTEIN
Keyword(s):  
Mammary Gland ◽  
Quantitative Difference ◽  
Late Pregnancy ◽  
Hydroxysteroid Dehydrogenase ◽  
Qualitative And Quantitative ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Rat Mammary Gland ◽  
Hydrogenase 3 ◽  
Qualitative Picture

SUMMARY The qualitative and quantitative metabolism of testosterone by rat mammary gland and hydrocarbon-induced mammary tumours has been investigated. These tissues contain a Δ4-5α-hydrogenase, 3-ketosteroid reductase and 17 β-hydroxysteroid dehydrogenase. The qualitative picture was the same in all the tissues studied but a quantitative difference was evident. In general, the tumours metabolized more steroid than mammary gland tissue taken from animals in late pregnancy. No conversion of testosterone to oestrone or oestradiol could be detected.

scholarly journals Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhipeng Zhang ◽  
Daijie Chen ◽  
Xubin Lu ◽  
Ruifeng Zhao ◽  
Zhi Chen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Antimicrobial Peptide ◽  
Expression Vector ◽  
Bovine Mastitis ◽  
Specific Expression ◽  
Mammary Gland Tissue ◽  
Gland Tissue

Bovine mastitis is perplexing the dairy industry since the initiation of intensive dairy farming, which has caused a reduction in the productivity of cows and an escalation in costs. The use of antibiotics causes a series of problems, especially the formation of bacterial antimicrobial resistance. However, there are limited antibiotic-free therapeutic strategies that can effectively relieve bacterial infection of bovine mammary glands. Hence, in this study, we constructed a mammary gland tissue-specific expression vector carrying the antimicrobial peptide of bovine-derived tracheal antimicrobial peptide (TAP) and evaluated it in both primary bovine mammary epithelial cells (pBMECs) and mice. The results showed that the vector driven by the β-lactoglobulin gene (BLG) promoter could efficiently direct the expression of TAP in pBMECs and the mammary gland tissue of mice. In addition, significant antibacterial effects were observed in both in vitro and in vivo experiments when introducing this vector to bovine-associated Staphylococcus aureus-treated pBMECs and mice, respectively. This study demonstrated that the mammary gland tissue-specific expression vector could be used to introduce antimicrobial peptide both in in vitro and in vivo and will provide a new therapeutic strategy in the treatment of bovine mastitis.

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