The Role of Hydration in Lysozyme Structure and Activity: Relevance in Protein Engineering and Design

1994 ◽  
pp. 349-365 ◽  
Author(s):  
P.L. Poole
2021 ◽  
Vol 22 (3) ◽  
pp. 1157
Author(s):  
Pablo Aza ◽  
Felipe de Salas ◽  
Gonzalo Molpeceres ◽  
David Rodríguez-Escribano ◽  
Iñigo de la Fuente ◽  
...  

Laccases secreted by saprotrophic basidiomycete fungi are versatile biocatalysts able to oxidize a wide range of aromatic compounds using oxygen as the sole requirement. Saccharomyces cerevisiae is a preferred host for engineering fungal laccases. To assist the difficult secretion of active enzymes by yeast, the native signal peptide is usually replaced by the preproleader of S. cerevisiae alfa mating factor (MFα1). However, in most cases, only basal enzyme levels are obtained. During directed evolution in S. cerevisiae of laccases fused to the α-factor preproleader, we demonstrated that mutations accumulated in the signal peptide notably raised enzyme secretion. Here we describe different protein engineering approaches carried out to enhance the laccase activity detected in the liquid extracts of S. cerevisiae cultures. We demonstrate the improved secretion of native and engineered laccases by using the fittest mutated α-factor preproleader obtained through successive laccase evolution campaigns in our lab. Special attention is also paid to the role of protein N-glycosylation in laccase production and properties, and to the introduction of conserved amino acids through consensus design enabling the expression of certain laccases otherwise not produced by the yeast. Finally, we revise the contribution of mutations accumulated in laccase coding sequence (CDS) during previous directed evolution campaigns that facilitate enzyme production.


ACS Catalysis ◽  
2015 ◽  
Vol 5 (2) ◽  
pp. 1078-1086 ◽  
Author(s):  
Begoña Puértolas ◽  
Álvaro Mayoral ◽  
Raul Arenal ◽  
Benjamín Solsona ◽  
Alaina Moragues ◽  
...  

1998 ◽  
Vol 49 (4) ◽  
pp. 382-384 ◽  
Author(s):  
S. Navon-Venezia ◽  
E. Banin ◽  
E. Z. Ron ◽  
E. Rosenberg

2014 ◽  
Vol 1 (4) ◽  
pp. 73-82
Author(s):  
Oleg Zhernokleyev

The paper highlights the role of communities of monks and nuns (the Third Orders) inthe structure and activity of the Ukrainian Greek Catholic Church (UGCC), which functionedunderground. It is emphasized that the historical roots of the Third Order are traced back to the13th century when spiritual life of specific lay communities was regulated by the Franciscan andthe Dominican Orders, and later, by the Carmelite Order. Between 1900 and 1930s lay communitiesof the UGCC became noticeably active. A characteristic example of their activity is the well knownRules for laypersons of the Basilian Order drawn by Metropolitan Archbishop Andrey Sheptytsky.In the 1970s (the Soviet time, when the UGCC worked underground), there appeared theRedemptorist and the Basilian Third Orders in Ivano-Frankivsk Oblast. Their members kept insafety different cult objects, held underground Divine Services, meetings, had spiritual practices,did catechesis, etc. On the whole, these lay communities had played an important role in theUGCC underground activity up till the late 1980s


Sign in / Sign up

Export Citation Format

Share Document