A modern, simple, and economical accessory for continuous L-format measurement of steady-state fluorescence anisotropy

2021 ◽  
pp. 114473
Author(s):  
Dairo A. Rendon ◽  
Juan L. Palacio
Keyword(s):  
Steady State ◽  
Fluorescence Anisotropy ◽  
Steady State Fluorescence

scholarly journals Effect of Nedocromil Sodium on Polymorphonuclear Leukocyte Plasma Membrane

1994 ◽  
Vol 3 (7) ◽  
pp. S21-S24 ◽  
Author(s):  
A. Kantar ◽  
N. Oggiano ◽  
P. L. Giorgi ◽  
G. V. Coppa ◽  
R. Gabbianelli ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Steady State ◽  
Membrane Fluidity ◽  
Fluorescence Anisotropy ◽  
Polymorphonuclear Leukocyte ◽  
Polymorphonuclear Leukocytes ◽  
Plasma Membranes ◽  
Nedocromil Sodium ◽  
Plasma Membrane Fluidity ◽  
Steady State Fluorescence

The effect of nedocromil sodium on the plasma membrane fluidity of polymorphonuclear leukocytes (PMNs) was investigated by measuring steady-state fluorescence anisotropy of 1-[4-trimethylammonium-phenyl]-6-phenyl- 1,3,5-hexatriene (TMA-DPH) incorporated in the membrane. Our results show that nedocromil sodium 300 μM significantly decreased membrane fluidity of PMNs. The decrease in membrane fluidity of PMNs induced by fMLP was abolished in the presence of nedocromil sodium. These data suggest that nedocromil sodium interferes with the plasma membranes of PMNs and modulates their activities.

scholarly journals Interactions of Ionic Liquids and Spirocyclic Compounds with Liposome Model Membranes. A Steady-State Fluorescence Anisotropy Study

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Antti H. Rantamäki ◽  
Wen Chen ◽  
Paulus Hyväri ◽  
Jussi Helminen ◽  
Gabriel Partl ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Steady State ◽  
Fluorescence Anisotropy ◽  
Active Compounds ◽  
Steady State Fluorescence ◽  
Structure Relation ◽  
Surface Active ◽  
Spirocyclic Compounds ◽  
Surface Active Compounds

AbstractUnderstanding the toxicity of ionic liquids (ILs) is crucial in the search of greener chemicals. By comparing in vivo toxicity and in vitro interactions determined between compounds and biomimetic lipid membranes, more detailed toxicity vs. structure relation can be obtained. However, determining the interactions between non-surface-active compounds and liposomes has been a challenging task. Organisational changes induced by ILs and IL-like spirocyclic compounds within 1,6-diphenyl-1,3,5-hexatriene-doped biomimetic liposomes was studied by steady-state fluorescence anisotropy technique. The extent of organisational changes detected within the liposome bilayers were compared to the toxicity of the compounds determined using Vibrio Fischeri bacteria. Four liposome compositions made of pure 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocoline (POPC) and mixtures of POPC, 1-palmitoyl-2-oleyl-sn-glycero-3-phosphoserine (POPS), and cholesterol (Chol) were tested as biomimetic models. Changes observed within the POPC/POPS/Chol 55:20:25 bilayers correlated the best with the toxicity results: ten out of twelve compounds followed the trend of increasing bilayer disorder – increasing toxicity. The study suggests that the toxicity of non-surface-active compounds is dependent on their ability to diffuse into the bilayers. The extent of bilayer’s organisational changes correlates rather well with the toxicity of the compounds. Highly sensitive technique, such as fluorescence anisotropy measurements, is needed for detecting subtle changes within the bilayer structures.

scholarly journals Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)

2013 ◽  
Vol 85 (3) ◽  
pp. 589-608 ◽  
Author(s):  
Marcel Ameloot ◽  
Martin vandeVen ◽  
A. Ulises Acuña ◽  
Bernard Valeur
Keyword(s):  
Steady State ◽  
Fluorescence Anisotropy ◽  
Fluorescence Polarization ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Steady State Fluorescence ◽  
Technical Report ◽  
Accuracy Of Measurements ◽  
Solution Methods ◽  
Systematic Effects

After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.

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