Rapid and simultaneous determination of salvianolic acid A and salvianolic acid B by complex chromatography and study on related mechanism of coordination

2008 ◽  
Vol 628 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Beibei Zhou ◽  
Jian Pan ◽  
Jia Wang
Keyword(s):  
Simultaneous Determination ◽  
Salvianolic Acid B ◽  
Salvianolic Acid ◽  
Salvianolic Acid A

Simultaneous Determination of Danshensu, Salvianolic Acid B, and Paeonol in ShuangDan Oral Liquid by HPLC

2013 ◽  
Vol 96 (1) ◽  
pp. 20-23 ◽  
Author(s):  
Hua Li ◽  
Siwang Wang ◽  
Yanhua Xie ◽  
Bangle Zhang ◽  
Jianbo Wang ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Control Method ◽  
Medicinal Preparation ◽  
Gradient Elution ◽  
Salvianolic Acid B ◽  
Array Detector ◽  
Column Temperature ◽  
Salvianolic Acid ◽  
Oral Liquid

Abstract An HPLC-photodiode array detector (PDAD) method was developed for simultaneous determination of danshensu, salvianolic acid B, and paeonol in a traditional Chinese medicinal preparation ShuangDan oral liquid, which is widely used to treat coronary heart disease in China. The samples were separated on a SinoChrom ODS–BP C18 column with the column temperature maintained at 30°C. The mobile phase was composed of methanol and 2% (v/v) glacial acetic acid at a flow rate of 1.0 mL/min. The separation was finished within 30 min using linear gradient elution. All calibration curves showed good linearity (r > 0.9997) within the test ranges. The method was simple, reliable, accurate, and specific. The intraday and interday precisions (RSD) were less than 0.85%. The recoveries were between 96.97 and 102.14%. The method was successfully applied to the determination of the three components in three commercial samples of ShuangDan oral liquid. The results indicated that the developed HPLC–PDAD assay could be readily utilized as a comprehensive quality control method for ShuangDan oral liquid.

scholarly journals Intervention Effect of Compatibility of Salvianolic Acid A & B on PDGF-C/PDGFR-α Signaling Pathway in Renal Fibrosis

2015 ◽  
Vol 4 (2) ◽  
pp. 13
Author(s):  
Bin Yu
Keyword(s):  
Signaling Pathway ◽  
Renal Fibrosis ◽  
Kidney Tissue ◽  
Renal Tissue ◽  
Salvianolic Acid B ◽  
Control Group ◽  
Salvianolic Acid ◽  
Salvianolic Acid A ◽  
Drug Compatibility ◽  
Renal Tubular

<strong>Objective</strong>: To explore the effect of salvianolic acid A &amp; B component molecules of drug compatibility on PDGF-c/PDGFR-α signaling pathway in renal fibrosis of rats. <strong>Methods</strong>: 40 male SPF SD rats were randomly divided into four groups: control group, salvianolic acid A group, salvianolic acid B group and salvianolic acid A + B group, with 10 rats in each group. Each group was treated for two weeks. After the intervention, samples were collected. And scores of HE and Masson was compared. The expression of PDGF-c/PDGFR-α in rental tissue in them was also tested and compared.<strong> Results</strong>: Compared with the control group, the score of HE and Masson in intervention groups was markedly decreased, and scores in salvianolic acid B group and salvianolic acid A + B group were reduced significantly (<em>p </em>&lt; 0.05); Compared with the control group, the expression of PDGF-c/PDGFR-α in rental tissue in intervention groups was lower(<em>p </em>&lt; 0.05), especially in salvianolic acid B group and salvianolic acid A + B group (<em>p </em>&lt; 0.05).<strong>Conclusion</strong>: salvianolic acid A &amp; B component molecules of drug compatibility could significantly improve the pathological changes in the kidney tissue of rats, suppress the expression of PDGF-c/PDGFR-α in renal tissue, and improve the renal function, renal tubular function and renal pathology.

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