“Recent advances on support materials for lipase immobilization and applicability as biocatalysts in inhibitors screening methods”-A review

2020 ◽  
Vol 1101 ◽  
pp. 9-22 ◽  
Author(s):  
Jia Liu ◽  
Run-Tian Ma ◽  
Yan-Ping Shi
Keyword(s):  
Screening Methods ◽  
Lipase Immobilization ◽  
Support Materials ◽  
Recent Advances

Optimum Conditions for Lipase Immobilization on Halloysitum Rubrum

2013 ◽  
Vol 864-867 ◽  
pp. 465-471
Author(s):  
Tao Deng ◽  
Jun Wei Xu ◽  
Li Huang ◽  
Tao Li ◽  
Xu Ya Yu
Keyword(s):  
Response Surface Methodology ◽  
Experimental Design ◽  
Immobilized Lipase ◽  
Candida Rugosa ◽  
Initial Activity ◽  
Optimum Conditions ◽  
Lipase Immobilization ◽  
Support Materials ◽  
Optimum Ph ◽  
Support Ratio

In this study, we use natural halloysitum rubrum as novel support materials to immobilize Candida rugosa lipase. The response surface methodology with a four-factor three-level Box-Behnken experimental design was used to evaluate the effects of immobilization parameters, such as pH (4.0 to 6.0), immobilization temperature (25 °C to 35 °C), enzyme/support ratio (0.1 to 0.3, w/w), and immobilization time (1 h to 2 h), on the activity of immobilized lipase. The optimum pH, temperature, enzyme/support ratio, and time for immobilized lipase activity (376.09 U/g) were 5.17, 29.65 °C, 0.3 (w/w), and 1.63 h, respectively. After 15 repeated uses, the immobilized lipase still retained 80% of its initial activity, which indicates good reusability.

scholarly journals Novel amphiphilic polyvinylpyrrolidone functionalized silicone particles as carrier for low-cost lipase immobilization

2018 ◽  
Vol 5 (6) ◽  
pp. 172368 ◽  
Author(s):  
Shan Zhang ◽  
Qianchun Deng ◽  
Ya Li ◽  
Mingming Zheng ◽  
Chuyun Wan ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Rational Design ◽  
Low Cost ◽  
Immobilized Lipase ◽  
High Catalytic Activity ◽  
Lipase Immobilization ◽  
X Ray ◽  
Support Materials ◽  
Steady Rate ◽  
Loading Amount

The high catalytic activity, specificity and stability of immobilized lipase have been attracting great interest. How to reduce the cost of support materials has always been a hot topic in this field. Herein, for the development of low-cost immobilized lipase, we demonstrate an amphiphilic polyvinylpyrrolidone (PVP) grafted on silicone particle (SP) surface materials (SP-PVP) with a rational design based on interfacial activation and solution polymerization. Meanwhile, hydrophilic pristine SP and hydrophobic polystyrene-corded silicone particles (SP-Pst) were also prepared for lipase immobilization. SP-PVP was characterized by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and thermogravimetry. Our results indicated that the lipase loading amount on the SP-PVP composites was about 215 mg of protein per gram. In the activity assay, the immobilized lipase SP-PVP@CRL exhibited higher catalysis activity and better thermostability and reusability than SP@CRL and SP-Pst@CRL. The immobilized lipase retained more than 54% of its initial activity after 10 times of re-use and approximately trended to a steady rate in the following cycles. By introducing the interesting amphiphilic polymer to this cheap and easily obtained SP surface, the relative performance of the immobilized lipase can be significantly improved, facilitating interactions between the low-cost support materials and lipase.

Recent advances in column support materials

1985 ◽  
Vol 13 (6) ◽  
pp. 1049-1052 ◽  
Author(s):  
JAN-CHRISTER JANSON
Keyword(s):  
Support Materials ◽  
Recent Advances

Glycoenzyme Tool Development: Principles, Screening Methods, and Recent Advances

2021 ◽  
Author(s):  
Tongxiao Cui ◽  
Yi Man ◽  
Feifei Wang ◽  
Shuyang Bi ◽  
Liang Lin ◽  
...  
Keyword(s):  
Screening Methods ◽  
Tool Development ◽  
Recent Advances

Recent Advances in High Dispersion Spectroscopy of Globular Cluster Stars

1988 ◽  
Vol 132 ◽  
pp. 525-530
Author(s):  
Raffaele G. Gratton
Keyword(s):  
Globular Cluster ◽  
Globular Clusters ◽  
High Dispersion ◽  
Photographic Material ◽  
Recent Advances ◽  
Metal Abundances ◽  
Ccd Detectors ◽  
Major Progress

The use CCD detectors has allowed a major progress in abundance derivations for globular cluster stars in the last years. Abundances deduced from high dispersion spectra now correlates well with other abundance indicators. I discuss some problems concerning the derivation of accurate metal abundances for globular clusters using high dispersion spectra from both the old photographic and the most recent CCD data. The discrepant low abundances found by Cohen (1980), from photographic material for M71 giants, are found to be due to the use of too high microturbulences.

Chelation-assisted transition metal-catalysed C–H chalcogenylations

2020 ◽  
Vol 7 (8) ◽  
pp. 1022-1060 ◽  
Author(s):  
Wenbo Ma ◽  
Nikolaos Kaplaneris ◽  
Xinyue Fang ◽  
Linghui Gu ◽  
Ruhuai Mei ◽  
...  
Keyword(s):  
Transition Metal ◽  
Recent Advances ◽  
Site Selectivity ◽  
Transition Metal Catalyzed ◽  
Metal Catalyzed

This review summarizes recent advances in C–S and C–Se formations via transition metal-catalyzed C–H functionalization utilizing directing groups to control the site-selectivity.

Nucleosome dynamics

2006 ◽  
Vol 73 ◽  
pp. 109-119 ◽  
Author(s):  
Chris Stockdale ◽  
Michael Bruno ◽  
Helder Ferreira ◽  
Elisa Garcia-Wilson ◽  
Nicola Wiechens ◽  
...  
Keyword(s):  
High Resolution ◽  
Chromatin Structure ◽  
Current Knowledge ◽  
Dynamic Properties ◽  
Structure And Dynamics ◽  
Nucleosome Dynamics ◽  
Sharp Focus ◽  
Recent Advances ◽  
Insight Into ◽  
Chromatin Structure And Dynamics

In the 30 years since the discovery of the nucleosome, our picture of it has come into sharp focus. The recent high-resolution structures have provided a wealth of insight into the function of the nucleosome, but they are inherently static. Our current knowledge of how nucleosomes can be reconfigured dynamically is at a much earlier stage. Here, recent advances in the understanding of chromatin structure and dynamics are highlighted. The ways in which different modes of nucleosome reconfiguration are likely to influence each other are discussed, and some of the factors likely to regulate the dynamic properties of nucleosomes are considered.

Recent Advances in the Treatment of Pulmonary Tuberculosis

1950 ◽  
Vol 34 (5) ◽  
pp. 1363-1380
Author(s):  
Theodore L. Badger ◽  
William E. Patton
Keyword(s):  
Pulmonary Tuberculosis ◽  
Recent Advances
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