Background:
Incoherent use of antibiotics has led toward resistance in MRSA, which is becoming multidrugresistant with high rate of virulence in the community and hospital settings.
Objective:
Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of
amoxicillin, Ag nanoparticles and chitosan which were prepared by one-step synthesis method and various characterizations
were performed.
Methods:
Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques while
agar well diffusion was used for hybrid composite spheres. The in vitro and cytotoxicity studies was done by skin abrasion
mouse model and MTT assay on RD cell respectively.
Results:
All isolates were resistant with the tested antibiotics except vancomycin. MIC against MRSA showed high
resistance with amoxicillin from 4 to 128 mg L-1. The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm
and 277 nm respectively. Morphology of spheres was uneven, varied, porous and irregular in SEM and Ag nanoparticles
presence and formation was also seen in micrograph. No substantial interface among drug, nanoparticles and polymer was
found in XRD and IR showed characteristic peaks of all compound in the formulation. The in vitro assay showed augmented
anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm). A significant reduction in microbial
burden (~6.5 log10 CFU ml-1) was seen in vivo with loaded hybrid composite spheres formulation. The MTT assay indicated
no potential cytotoxicity with hybrid composite spheres.
Conclusion:
Synergistic effect, amoxicillin, new hybrid formulation, anti-MRSA activity, composite spheres. nanoparticles.
