Preparation and characterization of gelatin/hyaluronic acid cryogels for adipose tissue engineering: In vitro and in vivo studies

2013 ◽  
Vol 9 (11) ◽  
pp. 9012-9026 ◽  
Author(s):  
Kun-Hung Chang ◽  
Han-Tsung Liao ◽  
Jyh-Ping Chen
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Hyaluronic Acid ◽  
In Vivo Studies ◽  
Adipose Tissue Engineering

The tissue-engineered breast

2021 ◽  
pp. 1115-1120
Author(s):  
Wayne Morrison
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stem Cell Differentiation ◽  
Vascular Pedicle ◽  
Human Trial ◽  
Fat Transfer ◽  
Adipose Tissue Engineering ◽  
Proof Of Principle

The adipose tissue engineering paradigm of in vitro cell-seeded scaffolds subsequently implanted in vivo failed because of inadequate vascularization. Consequently, entirely in vivo models of tissue engineering are being trialled where angiogenic growth is stimulated in unison with expansion of implanted cells and matrices. In animals, impressive amounts of fat and fibroblastic tissue have been grown by matrix induction of preadipocytes or by redirecting a vascular pedicle with fat into a sealed chamber space and proof of principle shown in a human trial. The models are cumbersome limiting clinical translation and currently direct fat transfer by injection is simpler. Unlike true tissue engineering there is, however, no net gain of tissue and even when ‘successful’ it is debated whether the graft survives or is replaced by newly regenerated adipocytes. Research focuses on stem cell differentiation, cell survival, and matrix and biomechanical manipulations.

Structural and rheological characterization of hyaluronic acid-based scaffolds for adipose tissue engineering

Biomaterials ◽  
2007 ◽  
Vol 28 (30) ◽  
pp. 4399-4408 ◽  
Author(s):  
A BORZACCHIELLO ◽  
L MAYOL ◽  
P RAMIRES ◽  
A PASTORELLO ◽  
C BARTOLO ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Hyaluronic Acid ◽  
Rheological Characterization ◽  
Adipose Tissue Engineering

scholarly journals Comparing Scaffold-Free and Fibrin-Based Adipose-Derived Stromal Cell Constructs for Adipose Tissue Engineering: An In Vitro and in Vivo Study

2012 ◽  
Vol 21 (10) ◽  
pp. 2283-2297 ◽  
Author(s):  
Femke Verseijden ◽  
Sandra J. Posthumus-Van Sluijs ◽  
Johan W. Van Neck ◽  
Stefan O. P. Hofer ◽  
Stevan E. R. Hovius ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stromal Cell ◽  
In Vivo Study ◽  
Adipose Tissue Engineering

scholarly journals Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1378
Author(s):  
Peyton Gibler ◽  
Jeffrey Gimble ◽  
Katie Hamel ◽  
Emma Rogers ◽  
Michael Henderson ◽  
...  
Keyword(s):  
Systematic Review ◽  
Adipose Tissue ◽  
Drug Discovery ◽  
3D Culture ◽  
Human Adipose Tissue ◽  
Culture Methods ◽  
Adipose Tissue Engineering ◽  
The Impact

Human adipose-derived stromal/stem cells (hASC) are widely used for in vitro modeling of physiologically relevant human adipose tissue. These models are useful for the development of tissue constructs for soft tissue regeneration and 3-dimensional (3D) microphysiological systems (MPS) for drug discovery. In this systematic review, we report on the current state of hASC culture and assessment methods for adipose tissue engineering using 3D MPS. Our search efforts resulted in the identification of 184 independent records, of which 27 were determined to be most relevant to the goals of the present review. Our results demonstrate a lack of consensus on methods for hASC culture and assessment for the production of physiologically relevant in vitro models of human adipose tissue. Few studies have assessed the impact of different 3D culture conditions on hASC adipogenesis. Additionally, there has been a limited use of assays for characterizing the functionality of adipose tissue in vitro. Results from this study suggest the need for more standardized culture methods and further analysis on in vitro tissue functionality. These will be necessary to validate the utility of 3D MPS as an in vitro model to reduce, refine, and replace in vivo experiments in the drug discovery regulatory process.

TRENDS AND CHALLENGES OF CARTILAGE TISSUE ENGINEERING

2009 ◽  
Vol 21 (03) ◽  
pp. 149-155 ◽  
Author(s):  
Hsu-Wei Fang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Growth Factors ◽  
Bone Cells ◽  
Cartilage Tissue Engineering ◽  
Bioactive Molecules ◽  
In Vivo Studies ◽  
Cartilage Tissue

Cartilage injuries may be caused by trauma, biomechanical imbalance, or degenerative changes of joint. Unfortunately, cartilage has limited capability to spontaneous repair once damaged and may lead to progressive damage and degeneration. Cartilage tissue-engineering techniques have emerged as the potential clinical strategies. An ideal tissue-engineering approach to cartilage repair should offer good integration into both the host cartilage and the subchondral bone. Cells, scaffolds, and growth factors make up the tissue engineering triad. One of the major challenges for cartilage tissue engineering is cell source and cell numbers. Due to the limitations of proliferation for mature chondrocytes, current studies have alternated to use stem cells as a potential source. In the recent years, a lot of novel biomaterials has been continuously developed and investigated in various in vitro and in vivo studies for cartilage tissue engineering. Moreover, stimulatory factors such as bioactive molecules have been explored to induce or enhance cartilage formation. Growth factors and other additives could be added into culture media in vitro, transferred into cells, or incorporated into scaffolds for in vivo delivery to promote cellular differentiation and tissue regeneration.Based on the current development of cartilage tissue engineering, there exist challenges to overcome. How to manipulate the interactions between cells, scaffold, and signals to achieve the moderation of implanted composite differentiate into moderate stem cells to differentiate into hyaline cartilage to perform the optimum physiological and biomechanical functions without negative side effects remains the target to pursue.

scholarly journals Tissue Engineering of Muscles and Cartilages Using Polyelectrolyte Hydrogels

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hyuck Joon Kwon
Keyword(s):  
Tissue Engineering ◽  
In Vivo Studies ◽  
Current Status ◽  
Polyelectrolyte Gel ◽  
Polyelectrolyte Gels ◽  
Joint Surfaces ◽  
Functional Replacement ◽  
And Cartilage

The prevalent nature of osteoarthritis that causes the erosion of joint surfaces and loss of mobility and muscle dystrophy that weakens the musculoskeletal system and hampers locomotion underlies the importance of developing functional replacement or regeneration of muscle and cartilage tissues. Polyelectrolyte gels have high potential as cellular scaffolds due to characteristic properties similar to biological matrixes. A number of in vitro and in vivo studies demonstrated that polyelectrolyte gels are useful for replacement and regeneration of muscle and cartilage tissues. In addition, it was also found that polyelectrolyte gels have high biocompatibility, durability, and resistance to biodegradation. Moreover, polyelectrolyte gels can overcome their drawbacks of mechanical behavior by introducing double network into the gel. This paper reviews the current status and recent progress of polyelectrolyte gel-based tissue engineering for repairs of muscle and cartilage tissues.

Mitochondrial Targeted Doxorubicin-Triphenylphosphonium Delivered by Hyaluronic Acid Modified and pH Responsive Nanocarriers to Breast Tumor: in Vitro and in Vivo Studies

2018 ◽  
Vol 15 (3) ◽  
pp. 882-891 ◽  
Author(s):  
Hui-Na Liu ◽  
Ning-Ning Guo ◽  
Tian-Tian Wang ◽  
Wang-Wei Guo ◽  
Meng-Ting Lin ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Breast Tumor ◽  
In Vivo Studies ◽  
Ph Responsive

scholarly journals Fabrication of Injectable, Porous Hyaluronic Acid Hydrogel Based on an In-Situ Bubble-Forming Hydrogel Entrapment Process

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1138
Author(s):  
Lixuan Wang ◽  
Shiyan Dong ◽  
Yutong Liu ◽  
Yifan Ma ◽  
Jingjing Zhang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Regenerative Medicine ◽  
In Vivo Studies ◽  
Injectable Hydrogels ◽  
Cell Behaviors ◽  
Porous Architecture ◽  
In Situ Hydrogel

Injectable hydrogels have been widely applied in the field of regenerative medicine. However, current techniques for injectable hydrogels are facing a challenge when trying to generate a biomimetic, porous architecture that is well-acknowledged to facilitate cell behaviors. In this study, an injectable, interconnected, porous hyaluronic acid (HA) hydrogel based on an in-situ bubble self-generation and entrapment process was developed. Through an amide reaction between HA and cystamine dihydrochloride activated by EDC/NHS, CO2 bubbles were generated and were subsequently entrapped inside the substrate due to a rapid gelation-induced retention effect. HA hydrogels with different molecular weights and concentrations were prepared and the effects of the hydrogel precursor solution’s concentration and viscosity on the properties of hydrogels were investigated. The results showed that HA10-10 (10 wt.%, MW 100,000 Da) and HA20-2.5 (2.5 wt.%, MW 200,000 Da) exhibited desirable gelation and obvious porous structure. Moreover, HA10-10 represented a high elastic modulus (32 kPa). According to the further in vitro and in vivo studies, all the hydrogels prepared in this study show favorable biocompatibility for desirable cell behaviors and mild host response. Overall, such an in-situ hydrogel with a self-forming bubble and entrapment strategy is believed to provide a robust and versatile platform to engineer injectable hydrogels for a variety of applications in tissue engineering, regenerative medicine, and personalized therapeutics.

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