scholarly journals Long-term nitrate removal in a denitrification wall

2011 ◽  
Vol 140 (3-4) ◽  
pp. 514-520 ◽  
Author(s):  
Lauren M. Long ◽  
Louis A. Schipper ◽  
Denise A. Bruesewitz
Water ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1298 ◽  
Author(s):  
Dedong Kong ◽  
Wenbing Li ◽  
Yale Deng ◽  
Yunjie Ruan ◽  
Guangsuo Chen ◽  
...  

An aerobic denitrifier was isolated from a long-term poly (3-hydroxybutyrate-co-3-hydroxyvalerate) PHBV-supported denitrification reactor that operated under alternate aerobic/anoxic conditions. The strain was identified as Marinobacter hydrocarbonoclasticus RAD-2 based on 16S rRNA-sequence phylogenetic analysis. Morphology was observed by scanning electron microscopy (SEM), and phylogenetic characteristics were analyzed with the API 20NE test. Strain RAD-2 showed efficient aerobic denitrification ability when using NO3−-N or NO2−-N as its only nitrogen source, while heterotrophic nitrification was not detected. The average NO3−-N and NO2−-N removal rates were 6.47 mg/(L·h)and 6.32 mg/(L·h), respectively. Single-factor experiments indicated that a 5:10 C/N ratio, 25–40 °C temperature, and 100–150 rpm rotation speed were the optimal conditions for aerobic denitrification. Furthermore, the denitrifying gene napA had the highest expression on a transcriptional level, followed by the denitrifying genes nirS and nosZ. The norB gene was found to have significantly low expression during the experiment. Overall, great aerobic denitrification ability makes the RAD-2 strain a potential alternative in enhancing nitrate management for marine recirculating aquaculture system (RAS) practices.


2005 ◽  
Vol 34 (4) ◽  
pp. 1270-1276 ◽  
Author(s):  
Louis A. Schipper ◽  
Gregory F. Barkle ◽  
Maja Vojvodic-Vukovic

2018 ◽  
Vol 79 (5) ◽  
pp. 975-984 ◽  
Author(s):  
Tatsuru Kamei ◽  
Rawintra Eamrat ◽  
Kenta Shinoda ◽  
Yasuhiro Tanaka ◽  
Futaba Kazama

Abstract Nitrate removal during anaerobic ammonium oxidation (anammox) treatment is a concern for optimization of the anammox process. This study demonstrated the applicability and long-term stability of the coupled anammox and hydrogenotrophic denitrification (CAHD) process as an alternative method for nitrate removal. Laboratory-scale fixed bed anammox reactors (FBR) supplied with H2 to support denitrification were operated under two types of synthetic water. The FBRs showed simultaneous NH4-N and NO3-N removal, indicating that the CAHD process can support NO3-N removal during the anammox process. Intermittent H2 supply (e.g. 5 mL/min for a 1-L reactor, 14/6-min on/off cycle) helped maintain the CAHD process without deteriorating its performance under long-term operation and resulted in a nitrogen removal rate of 0.21 kg-N/m3/d and ammonium, nitrate, and dissolved inorganic nitrogen removal efficiencies of 73.4%, 80.4%, and 77%, respectively. The microbial community structure related to the CAHD process was not influenced by changes in influent water quality, and included the anammox bacteria ‘Candidatus Jettenia’ and a Sulfuritalea hydrogenivorans-like species as the dominant bacteria even after long-term reactor operation, suggesting that these bacteria are key to the CAHD process. These results indicate that the CAHD process is a promising method for enhancing the efficiency of anammox process.


2004 ◽  
Vol 69 (3-4) ◽  
pp. 263-279 ◽  
Author(s):  
Louis A. Schipper ◽  
Gregory F. Barkle ◽  
John C. Hadfield ◽  
Maja Vojvodic-Vukovic ◽  
Craig P. Burgess

2017 ◽  
Vol 106 ◽  
pp. 523-531 ◽  
Author(s):  
Rui Li ◽  
Chuanping Feng ◽  
Beidou Xi ◽  
Nan Chen ◽  
Yu Jiang ◽  
...  

Water ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 3608
Author(s):  
Fabio Manca ◽  
Carla Wegscheidl ◽  
Rhianna Robinson ◽  
Suzette Argent ◽  
Christopher Algar ◽  
...  

In Australia, declining water quality in the Great Barrier Reef (GBR) is a threat to its marine ecosystems and nitrate (NO3−) from sugar cane-dominated agricultural areas in the coastal catchments of North Queensland is a key pollutant of concern. Woodchip bioreactors have been identified as a potential low-cost remediation technology to reduce the NO3− runoff from sugar cane farms. This study aimed to trial different designs of bioreactors (denitrification walls and beds) to quantify their NO3− removal performance in the distinct tropical climates and hydrological regimes that characterize sugarcane farms in North Queensland. One denitrification wall and two denitrification beds were installed to treat groundwater and subsurface tile-drainage water in wet tropics catchments, where sugar cane farming relies only on rainfall for crop growth. Two denitrification beds were installed in the dry tropics to assess their performance in treating irrigation tailwater from sugarcane. All trialled bioreactors were effective at removing NO3−, with the beds exhibiting a higher NO3− removal rate (NRR, from 2.5 to 7.1 g N m−3 d−1) compared to the wall (0.15 g N m−3 d−1). The NRR depended on the influent NO3− concentration, as low influent concentrations triggered NO3− limitation. The highest NRR was observed in a bed installed in the dry tropics, with relatively high and consistent NO3− influent concentrations due to the use of groundwater, with elevated NO3−, for irrigation. This study demonstrates that bioreactors can be a useful edge-of-field technology for reducing NO3− in runoff to the GBR, when sited and designed to maximise NO3− removal performance.


1992 ◽  
Vol 26 (3-4) ◽  
pp. 535-544
Author(s):  
M. M. de Mendonca ◽  
J. Silverstein ◽  
N. E. Cook

The long- and short-term effects of hydraulic loading were investigated in a pilot packed tower bioreactor used for denitrifying a groundwater used for drinking water. Field experiments were conducted at hydraulic loading rates of 3.63 and 7.24 m3/hr/m2. The long-term (21 day average) performance of the denitrification reactor and the short-term response (48 hour) to regular air scour are reported here. In summary, an increase in hydraulic loading resulted in a long-term increase in the half-order denitrification rate constant, so that, on average, nitrate removal was maintained in the 5.2 m tower. At the lower hydraulic loading rate (3.63 m3/hr/m2) denitrification performance in the first 48 hours after air scour was maintained; however at twice the hydraulic loading, several days were required to restore the biofilm performance to long-term average levels.


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