Peroxisome Proliferator-Activated Receptor-γ Knockdown Impairs Bone Morphogenetic Protein-2–Induced Critical-Size Bone Defect Repair

The best way in which to prepare scaffolds with good biological properties is an urgent problem in the field of tissue engineering. In this paper we discuss the preparation of nano-hydroxyapatite scaffold of recombinant human bone morphogenetic protein-2 (rhBMP-2) and its application in bone defect repair. rhBMP-2 reagent was dissolved in 1 mol/L sodium dihydrogen phosphate solution, and the rhBMP-2 solution was added to the nano-hydroxyapatite artificial bone with a 100 μL glass micro dropper at the rate of 10 drops/min to obtain Nano-HA/rhBMP-2 composite artificial bone. In in vivo experiments, rabbits were fixed on an operating table, a 2 cm longitudinal incision was made in the middle part of the radial forearm, and the radius was cut with a wire saw and periosteum, 2.5 cm away from the distal radius. After washing the wound with normal saline, Adv-hBMP-2/MC3T3-E1 nano-HA composite artificial bone, MC3T3-E1 nan-HA composite artificial bone, or Nano-HA artificial bone were implanted in different groups. The artificial bone scaffold prepared in this study has a stronger ability to repair bone defects than the alternatives, and is a promising prospect for the clinical treatment of bone defects.

Download Full-text

Acceleration of bone formation using in situ-formed hyaluronan-hydrogel containing bone morphogenetic protein-2 in a mouse critical size bone defect model

Bio-Medical Materials and Engineering ◽

10.3233/bme-201172 ◽

2021 ◽

pp. 1-9

Author(s):

Shintaro Shoji ◽

Kentaro Uchida ◽

Ryo Tazawa ◽

Wataru Saito ◽

Akiyoshi Kuroda ◽

...

Keyword(s):

Bone Formation ◽

Bone Morphogenetic Protein ◽

Bone Defect ◽

Bone Repair ◽

Critical Size ◽

Bone Morphogenetic Protein 2 ◽

Micro Computed Tomography ◽

Defect Model ◽

Morphogenetic Protein

BACKGROUND: An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHGs). IFHGs may also be ideal carrier materials for bone repair, although their ability to carry bone morphogenetic protein-2 (BMP2) has yet to be examined. OBJECTIVE: We examined the effectiveness of an IFHG made of hyaluronan (IFHG-HA) containing BMP2 for promoting bone formation in a mouse critical size bone defect model. METHODS: C57/BL6J mice received a 2-mm femoral critical-sized bone defect before being randomly assigned to one of the following treatment groups (n = 6): control (no treatment), IFHG-HA only, PBS with BMP2, and IFHG-HA with BMP2. X-ray radiographs were utilized to track new bone formation, and micro-computed tomography and histological examination were performed on new bone formed at the bone defect site two weeks after surgery. RESULTS: Mice treated with PBS with BMP2 and IFHG-HA with BMP2 had greater bone volume (BV) and bone mineral content (BMC) than those receiving control, and successfully achieved consolidation. Mice treated with IFHG-HA with BMP2 had significantly higher BV and BMC than those treated with PBS with BMP2. CONCLUSIONS: IFHG-HA may be an effective carrier for BMP2 to enable delivery for bone defect repair.

Download Full-text

Enhanced Bone Regeneration at a Segmental Bone Defect by Controlled Release of Bone Morphogenetic Protein-2 from a Biodegradable Hydrogel

Tissue Engineering ◽

10.1089/ten.2006.12.ft-68 ◽

2006 ◽

Vol 0 (0) ◽

pp. 060511071910001

Author(s):

Masaya Yamamoto ◽

Yoshitake Takahashi ◽

Yasuhiko Tabata

Keyword(s):

Controlled Release ◽

Bone Regeneration ◽

Bone Morphogenetic Protein ◽

Bone Defect ◽

Bone Morphogenetic Protein 2 ◽

Segmental Bone Defect ◽

Morphogenetic Protein ◽

Biodegradable Hydrogel ◽

Segmental Bone

Download Full-text

Thiol-acrylate nanocomposite foams for critical size bone defect repair: A novel biomaterial

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.34651 ◽

2013 ◽

Vol 101 (12) ◽

pp. 3531-3541 ◽

Cited By ~ 17

Author(s):

Leah Garber ◽

Cong Chen ◽

Kameron V. Kilchrist ◽

Christopher Bounds ◽

John A. Pojman ◽

...

Keyword(s):

Bone Defect ◽

Critical Size ◽

Bone Defect Repair ◽

Nanocomposite Foams ◽

Defect Repair

Download Full-text

Critical-size bone defect repair using amniotic fluid stem cell/collagen constructs: Effect of oral ferutinin treatment in rats

Life Sciences ◽

10.1016/j.lfs.2014.10.020 ◽

2015 ◽

Vol 121 ◽

pp. 174-183 ◽

Cited By ~ 15

Author(s):

Manuela Zavatti ◽

Laura Bertoni ◽

Tullia Maraldi ◽

Elisa Resca ◽

Francesca Beretti ◽

...

Keyword(s):

Stem Cell ◽

Amniotic Fluid ◽

Bone Defect ◽

Critical Size ◽

Bone Defect Repair ◽

Defect Repair ◽

Amniotic Fluid Stem Cell

Download Full-text

Heparin increases the osteogenic effect of recombinant human bone morphogenetic protein-2 in the rabbit bone defect model

Animal Cells and Systems ◽

10.1080/19768354.2015.1087429 ◽

2015 ◽

Vol 19 (5) ◽

pp. 312-320 ◽

Cited By ~ 3

Author(s):

Ju Yeon Ban ◽

Sang Wook Kang ◽

Gyeong-Ju Park

Keyword(s):

Bone Morphogenetic Protein ◽

Bone Defect ◽

Human Bone ◽

Bone Morphogenetic Protein 2 ◽

Defect Model ◽

Morphogenetic Protein ◽

Human Bone Morphogenetic Protein ◽

Rabbit Bone ◽

Osteogenic Effect ◽

Recombinant Human Bone

Download Full-text

Effect of Stromal Vascular Fraction on Fracture Healing with Bone Defects by Examination of Bone Morphogenetic Protein-2 Biomarkers in Murine Model

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.7385 ◽

2021 ◽

Vol 9 (A) ◽

pp. 1132-1136

Author(s):

Respati S. Dradjat ◽

Panji Sananta ◽

Rizqi Daniar Rosandi ◽

Lasa Dhakka Siahaan

Keyword(s):

Bone Formation ◽

Bone Morphogenetic Protein ◽

Bone Defect ◽

Murine Model ◽

Healing Process ◽

Stromal Vascular Fraction ◽

Bone Defects ◽

The Body ◽

Bone Morphogenetic Protein 2 ◽

Morphogenetic Protein

BACKGROUND: Fractures and segmental bone defects are a significant cause of morbidity and a source of a high economic burden in healthcare. A severe bone defect (3 mm in murine model) is a devastating condition, which the bone cannot heal naturally despite surgical stabilization and usually requires further surgical intervention. The stromal vascular fraction (SVF) contains a heterogeneous collection of cells and several components, primarily: MSCs, HSCs, Treg cells, pericytic cells, AST cells, extracellular matrix, and complex microvascular beds (fibroblasts, white blood cells, dendritic cells, and intra-adventitial smooth muscular-like cells). Bone morphogenetic protein (BMP) is widely known for their important role in bone formation during mammalian development and confers a multifunctional role in the body, which has potential for therapeutic use. Studies have shown that BMPs play a role in the healing of large size bone defects. AIM: In this study, researchers aim to determine the effect of administering SVF from adipose tissue on the healing process of bone defects assessed based on the level biomarker of BMP-2. MATERIALS AND METHODS: This was an animal study involving 12 Wistar strain Rattus norvegivus. They were divided into three groups: Negative group (normal rats), positive group (rats with bone defect without SVF application), and SVF group (rats with bone defect with SVF application). After 30 days, the rats were sacrificed; the biomarkers that were evaluated are BMP-2. This biomarker was quantified using ELISA. RESULTS: BMP-2 biomarker expressions were higher in the SVF application group than in the group without SVF. All comparisons of the SVF group and positive control group showed significant differences (p = 0.026). CONCLUSION: SVF application could aid the healing process in a murine model with bone defect marked by the increased level of BMP-2 as a bone formation marker.

Download Full-text

Association Between BMP2 Functional Polymorphisms and Sheep Tail Type

Animals ◽

10.3390/ani10040739 ◽

2020 ◽

Vol 10 (4) ◽

pp. 739

Author(s):

Zengkui Lu ◽

Jianbin Liu ◽

Jilong Han ◽

Bohui Yang

Keyword(s):

Tail Length ◽

Cellular Level ◽

Fat Deposition ◽

Bone Morphogenetic Protein 2 ◽

Peroxisome Proliferator ◽

Nucleotide Polymorphisms ◽

Peroxisome Proliferator Activated Receptor ◽

Functional Polymorphisms ◽

Genotype Frequencies ◽

Morphogenetic Protein

Bone morphogenetic protein 2 (BMP2) is strongly selected in both fat-tailed and thin-tailed sheep and may be a candidate gene for sheep tail type selection. However, the mechanism of action of BMP2 in sheep tail fat deposition remains unclear. This study investigated genetic variation and haplotype combinations of the BMP2 gene in sheep with different tail types, aiming to reveal the molecular mechanism of BMP2 in sheep tail fat deposition. We detected a total of three single nucleotide polymorphisms (SNPs) (g.48401619 T > A, g.48401272 C > A, and g.48401136 C > T) among 533 sheep. The alleles and genotype frequencies of these SNPs were in Hardy–Weinberg equilibrium and showed significant correlations with tail length. Linkage disequilibrium existed between the g.48401272 C > A and g.48401136 C > T sites, where CACT was the predominant genotype. At the cellular level, the expression levels of peroxisome proliferator-activated receptor gamma (PPARγ) and lipoprotein lipase (LPL) were upregulated after BMP2 overexpression; there were significantly higher levels of PPARγ than controls at 0 d and 1 d, and of LPL than controls at 1 d and 7 d. These results indicate that the BMP2 gene may participate in sheep tail fat deposition and could be used for molecular-marker-assisted selection of sheep tail type.

Download Full-text