Poly(aspartic acid) Biohydrogel as the Base of a New Hybrid Conducting Material

In the present study, a composite made of conducting polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), and a biodegradable hydrogel of poly(aspartic acid) (PASP) were electrochemically interpenetrated with poly(hydroxymethyl-3,4-ethylenedioxythiophene) (PHMeDOT) to prepare a new interpenetrated polymer network (IPN). Different cross-linker and PEDOT MPs contents, as well as different electropolymerization times, were studied to optimize the structural and electrochemical properties. The properties of the new material, being electrically conductive, biocompatible, bioactive, and biodegradable, make it suitable for possible uses in biomedical applications.

Influence of Bentonite Nanoparticles on Properties of PVP-CMC-Gums Biodegradable Hydrogel Films for Biomedical Applications

Using polymer daily becomes increasingly extensive; the many characteristics of hydrogel lead to a wide range of uses, particularly in biomedical applications. Hydrogel films were made from a variety of materials in this investigation. Casting techniques and room temperature drying were used to make PVP- CMC- Gums films based on hydrogels, however, the effects of adding bentonite clay were needed. SEM, FTIR, XRD, TGA, swelling, solubility, contact angle, and a variety of other studies were used to illustrate and analyze a variety of physical, mechanical, thermal, and many characteristics. The major findings revealed new peaks, which indicate the creation of cross-linking bonds, which are the primary cause of capsulation and release characteristics, indicating that these films might be utilized in drug delivery and a variety of other applications. The PCXB film has the best color, surface hydrophobicity, solubility, and swelling properties, while the PCGB film has the greatest biodegradability and permeability results, and both films have strong thermal, mechanical, and releasing properties. As a result, adding bentonite clay to hydrogel films improves all of their characteristics, making them suitable for a variety of biomedical applications such as dentistry root filling, tissue engineering, contact lenses, and bandages.

The Synergic Effect of Whey-Based Hydrogel Amendment on Soil Water Holding Capacity and Availability of Nutrients for More Efficient Valorization of Dairy By-Products

Agricultural production is influenced by the water content in the soil and the availability of nutrients. Recently, changes in the quantity and seasonal water availability are expected to impact agriculture due to climate change. This study aimed to test an agricultural product with promising properties to improve soil quality and water-holding capacity during agricultural application. Most of the traditional hydrogels are low-biodegradable synthetic materials with under-researched long-term fate in field soil conditions. The novel, biodegradable hydrogel made from acid whey and cellulose derivatives cross-linked with citric acid was used. The soil-improving effects were tested under controlled experimental conditions with the sandy artificial soil consisting of 10% finely ground sphagnum peat, 20% kaolinite clay, and 70% quartz sand. Soil pH, the content of organic carbon (Cox), total nitrogen (N), available forms of the essential macronutrients (P, K, Ca, and Mg), the cation exchange capacity (CEC), the maximum water capacity (MWC) and water holding capacity (WHC) were determined. The results showed a positive effect on water retention and basic soil properties after the different levels of hydrogel had been introduced into the soil. Generally, the addition of whey-based hydrogel increases the available nutrients concentration and water retention in soil.

Innovative Eco-Friendly Hydrogel Film for Berberine Delivery in Skin Applications

Hydrogel formulations (masks or patches, without tissue support) represent the new frontier for customizable skin beauty and health. The employment of these materials is becoming popular in wound dressing, to speed up the healing process while protecting the affected area, as well as to provide a moisturizing reservoir, control the inflammatory process and the onset of bacterial development. Most of these hydrogels are acrylic-based at present, not biodegradable and potentially toxic, due to acrylic monomers residues. In this work, we selected a new class of cellulose-derived and biodegradable hydrogel films to incorporate and convey an active compound for dermatological issues. Films were obtained from a combination of different polysaccharides and clays, and berberine hydrochloride, a polyphenolic molecule showing anti-inflammatory, immunomodulatory, antibacterial and antioxidant properties, was chosen and then embedded in the hydrogel films. These innovative hydrogel-based systems were characterized in terms of water uptake profile, in vitro cytocompatibility and skin permeation kinetics by Franz diffusion cell. Berberine permeation fitted well to Korsmeyer–Peppas kinetic model and achieved a release higher than 100 µg/cm2 within 24 h. The latter study, exploiting a reliable skin model membrane, together with the biological assessment, gained insights into the most promising formulation for future investigations.

GSK3 Inhibitor-Induced Dentinogenesis Using a Hydrogel

Small-molecule drugs targeting glycogen synthase kinase 3 (GSK3) as inhibitors of the protein kinase activity are able to stimulate reparative dentine formation. To develop this approach into a viable clinical treatment for exposed pulp lesions, we synthesized a novel, small-molecule noncompetitive adenosine triphosphate (ATP) drug that can be incorporated into a biodegradable hydrogel for placement by syringe into the tooth. This new drug, named NP928, belongs to the thiadiazolidinone (TDZD) family and has equivalent activity to similar drugs of this family such as tideglusib. However, NP928 is more water soluble than other TDZD drugs, making it more suitable for direct delivery into pulp lesions. We have previously reported that biodegradable marine collagen sponges can successfully deliver TDZD drugs to pulp lesions, but this involves in-theater preparation of the material, which is not ideal in a clinical context. To improve surgical handling and delivery, here we incorporated NP928 into a specifically tailored hydrogel that can be placed by syringe into a damaged tooth. This hydrogel is based on biodegradable hyaluronic acid and can be gelled in situ upon dental blue light exposure, similarly to other common dental materials. NP928 released from hyaluronic acid–based hydrogels upregulated Wnt/β-catenin activity in pulp stem cells and fostered reparative dentine formation compared to marine collagen sponges delivering equivalent concentrations of NP928. This drug-hydrogel combination has the potential to be rapidly developed into a therapeutic procedure that is amenable to general dental practice.

Promoting Neuronal Outgrowth Using Ridged Scaffolds Coated with Extracellular Matrix Proteins

Spinal cord injury (SCI) results in cell death, demyelination, and axonal loss. The spinal cord has a limited ability to regenerate, and current clinical therapies for SCI are not effective in helping promote neurologic recovery. We have developed a novel scaffold biomaterial that is fabricated from the biodegradable hydrogel oligo(poly(ethylene glycol)fumarate) (OPF). We have previously shown that positively charged OPF scaffolds (OPF+) in an open spaced, multichannel design can be loaded with Schwann cells to support axonal generation and functional recovery following SCI. We have now developed a hybrid OPF+ biomaterial that increases the surface area available for cell attachment and that contains an aligned microarchitecture and extracellular matrix (ECM) proteins to better support axonal regeneration. OPF+ was fabricated as 0.08 mm thick sheets containing 100 μm high polymer ridges that self-assemble into a spiral shape when hydrated. Laminin, fibronectin, or collagen I coating promoted neuron attachment and axonal outgrowth on the scaffold surface. In addition, the ridges aligned axons in a longitudinal bipolar orientation. Decreasing the space between the ridges increased the number of cells and neurites aligned in the direction of the ridge. Schwann cells seeded on laminin coated OPF+ sheets aligned along the ridges over a 6-day period and could myelinate dorsal root ganglion neurons over 4 weeks. This novel scaffold design, with closer spaced ridges and Schwann cells, is a novel biomaterial construct to promote regeneration after SCI.