scholarly journals Label-Free Quantitative Phase Imaging Reveals Spatial Heterogeneity of Extracellular Vesicles in Selected Colon Disorders

2021 ◽  
Author(s):  
Łukasz Zadka ◽  
Igor Buzalewicz ◽  
Agnieszka Ulatowska-Jarża ◽  
Agnieszka Rusak ◽  
Maria Kochel ◽  
...  
Keyword(s):  
Spatial Heterogeneity ◽  
Extracellular Vesicles ◽  
Phase Imaging ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

scholarly journals Reagent- and actuator-free analysis of individual erythrocytes using three-dimensional quantitative phase imaging and capillary microfluidics

2021 ◽  
Author(s):  
DongHun Ryu ◽  
Hyeono Nam ◽  
Jessie Sungyun Jeon ◽  
YongKeun Park
Keyword(s):  
Blood Cells ◽  
Histopathological Examination ◽  
Point Of Care ◽  
Hemoglobin Concentration ◽  
Biochemical Properties ◽  
Phase Imaging ◽  
Sample Treatment ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase

Histopathological examination of blood cells plays a crucial role in the diagnosis of various diseases. However, it involves time-consuming and laborious staining procedures required for microscopic review by medical experts and is not directly applicable for point-of-care diagnosis in resource-limited locations. This study reports a dilution-, actuation- and label-free method for the analysis of individual red blood cells (RBCs) using a capillary microfluidic device and quantitative phase imaging. Blood, without any sample treatment, is directly loaded into a micrometer-thick channel such that it forms a quasi-monolayer inside the channel. The morphological and biochemical properties of RBCs, including hemoglobin concentration, hemoglobin content, and corpuscular volume, were retrieved using the refractive index tomograms of individual RBCs measured using 3D quantitative phase imaging. The deformability of individual RBCs was also obtained by measuring the dynamic membrane fluctuations. The proposed framework applies to other imaging modalities and biomedical applications, facilitating rapid and cost-effective diagnosis and prognosis of diseases.

scholarly journals Integrative quantitative-phase and airy light-sheet imaging

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
N. R. Subedi ◽  
P. S. Jung ◽  
E. L. Bredeweg ◽  
S. Nemati ◽  
S. E. Baker ◽  
...  
Keyword(s):  
Evolutionary Biology ◽  
Imaging System ◽  
Light Sheet ◽  
Live Cells ◽  
Phase Imaging ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Light Sheet Microscopy ◽  
Order Of Magnitude

AbstractLight-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free recognition of cells and organelles, and quantifies their number-density that, thermodynamically, is more representative of metabolism than size. Here, we report the fusion of these two imaging modalities onto a standard inverted microscope that retains compatibility with microfluidics and open-source software for image acquisition and processing. An accelerating Airy-beam light-sheet critically enabled imaging areas that were greater by more than one order of magnitude than a Gaussian beam illumination and matched exactly those of quantitative-phase imaging. Using this integrative imaging system, we performed a demonstrative multivariate investigation of live-cells in microfluidics that unmasked that cellular noise can affect the compartmental localization of metabolic reactions. We detail the design, assembly, and performance of the integrative imaging system, and discuss potential applications in biotechnology and evolutionary biology.

scholarly journals Visualization and label-free quantification of microfluidic mixing using quantitative phase imaging

2017 ◽  
Vol 56 (22) ◽  
pp. 6341 ◽  
Author(s):  
GwangSik Park ◽  
Dongsik Han ◽  
GwangSu Kim ◽  
Seungwoo Shin ◽  
Kyoohyun Kim ◽  
...  
Keyword(s):  
Phase Imaging ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Microfluidic Mixing ◽  
Quantitative Phase ◽  
Label Free Quantification ◽  
Free Quantification
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