The effect of cysteamine hydrochloride and nitrate supplementation on in-vitro and in-vivo methane production and productivity of cattle

AbstractBackgroundThe red macroalgae Asparagopsis taxiformis is a potent natural supplement for reducing methane production from cattle. A. taxiformis contains several anti-methanogenic compounds including bromoform that inhibits directly methanogenesis. The positive and adverse effects of A. taxiformis on the rumen microbiota are dose-dependent and operate in a dynamic fashion. It is therefore key to characterize the dynamic response of the rumen microbial fermentation for identifying optimal conditions on the use of A. taxiformis as a dietary supplement for methane mitigation. Accordingly, the objective of this work was to model the effect of A. taxiformis supplementation on the rumen microbial fermentation under in vitro conditions. We adapted a published mathematical model of rumen microbial fermentation to account for A. taxiformis supplementation. We modelled the impact of A. taxiformis on the fermentation and methane production by two mechanisms, namely (i) direct inhibition of the growth rate of methanogenesis by bromoform and (ii) hydrogen control on sugars utilization and on the flux distribution towards volatile fatty acids production. We calibrated our model using a multi-experiment estimation approach that integrated experimental data with six macroalgae supplementation levels from a published in vitro study assessing the dose-response impact of A. taxiformis on rumen fermentation.Resultsour model captured satisfactorily the effect of A. taxiformis on the dynamic profile of rumen microbial fermentation for the six supplementation levels of A. taxiformis with an average determination coefficient of 0.88 and an average coefficient of variation of the root mean squared error of 15.2% for acetate, butyrate, propionate, ammonia and methane.Conclusionsour results indicated the potential of our model as prediction tool for assessing the impact of additives such as seaweeds on the rumen microbial fermentation and methane production in vitro. Additional dynamic data on hydrogen and bromoform are required to validate our model structure and look for model structure improvements. We are working on model extensions to account for in vivo conditions. We expect this model development can be useful to help the design of sustainable nutritional strategies promoting healthy rumen function and low environmental footprint.

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Effects of Geraniol and Camphene on in Vitro Rumen Fermentation and Methane Production

Scientia Agriculturae Bohemica ◽

10.1515/sab-2017-0012 ◽

2017 ◽

Vol 48 (2) ◽

pp. 63-69

Author(s):

M. Joch ◽

V. Kudrna ◽

B. Hučko

Keyword(s):

Methane Emission ◽

Methane Production ◽

Dry Matter ◽

Volatile Fatty Acids ◽

Rumen Fluid ◽

Positive Control ◽

Rumen Fermentation ◽

Dry Matter Digestibility

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.

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In vitro gas and methane production in rumen fluid from dairy cows fed grass silages differing in plant maturity, compared to in vivo data

Journal of Animal Physiology and Animal Nutrition ◽

10.1111/jpn.12898 ◽

2018 ◽

Vol 102 (4) ◽

pp. 843-852 ◽

Cited By ~ 1

Author(s):

F. M. Macome ◽

W. F. Pellikaan ◽

W. H. Hendriks ◽

D. Warner ◽

J. T. Schonewille ◽

...

Keyword(s):

Dairy Cows ◽

Methane Production ◽

Rumen Fluid ◽

Plant Maturity

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Comparison of In vivo and In vitro Techniques for Methane Production from Ruminant Diets

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.2007.1049 ◽

2007 ◽

Vol 20 (7) ◽

pp. 1049-1056 ◽

Cited By ~ 14

Author(s):

Raghavendra Bhatta ◽

K. Tajima ◽

N. Takusari ◽

K. Higuchi ◽

O. Enishi ◽

...

Keyword(s):

Methane Production ◽

In Vitro Techniques ◽

Ruminant Diets

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Effect of 9,10-Anthraquinone on rumen methane production as studied in vitro and in vivo

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200012813 ◽

2003 ◽

Vol 2003 ◽

pp. 122-122

Author(s):

V. Fievez ◽

B. Vlaeminck ◽

W. Steinberg ◽

I. Immig ◽

D. Demeyer

Keyword(s):

Fatty Acid ◽

Methane Production ◽

Volatile Fatty Acid ◽

Rumen Fermentation ◽

Dose Effect ◽

Total Volatile ◽

Total Volatile Fatty Acid

In vitro supplementation of 0.05% [on a substrate basis (wt/wt)] - but not of 0.01% - of 9,10-Anthraquinone (AQ) inhibited rumen methanogenesis, reduced total volatile fatty acid (VFA) concentrations and molar proportions of acetate (Acet), increased proportions of propionate (Prop) and butyrate (But) and resulted sometimes in H2 accumulation (Garcia-Lopez et al., 1996). In vivo administration of high amounts of AQ [5% on a substrate basis (wt/wt)] to lambs depressed CH4 and increased H2 concentrations in ruminal gases during the complete 19 days of administration, whereas original concentrations were re-installed within 6 days after the removal of AQ from the diet (Kung et al., 1996). In this experiment we aimed to study the dose effect of AQ on in vitro rumen fermentation and modifications to rumen fermentation when administering 0.05% of AQ in vivo.

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The impact of hexose partitioning in sheep in vivo

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200005391 ◽

2001 ◽

Vol 2001 ◽

pp. 157-157 ◽

Cited By ~ 3

Author(s):

A. R. Moss ◽

C. J. Newbold ◽

D.I. Givens

Keyword(s):

Methane Production ◽

Short Chain Fatty Acids ◽

Water Soluble ◽

Soluble Carbohydrate ◽

Carbohydrate Source ◽

Fermentation Products ◽

Water Soluble Carbohydrate ◽

The Impact

Methane production represents an important sink for hydrogen within the rumen Beever (1993) suggested that the partitioning of fermentable dry matter (DM) between microbial synthesis and fermentation products would alter the pattern of hydrogen production and hence methanogenesis. This hypothesis was investigated in vitro using a range of diets varying in carbohydrate source (Moss et al., 2000). Methane production (moles) increased as the proportion of DM fermented to short chain fatty acids (SCFA) increased and this was related to decreasing water soluble carbohydrate (WSC) to cell wall (NDF) ratio of the diet. The objectives of the current study was to design diets with a range of WSC:NDF ratios and to measure the impact on hexose partitioning and methane production in sheep in vivo.

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Effects of plants and essential oils on ruminal in vitro batch culture methane production and fermentation

Canadian Journal of Animal Science ◽

10.4141/cjas2012-059 ◽

2012 ◽

Vol 92 (3) ◽

pp. 395-408 ◽

Cited By ~ 3

Author(s):

J. A. Tekippe ◽

A. N. Hristov ◽

K. S. Heyler ◽

V. D. Zheljazkov ◽

J. F. S. Ferreira ◽

...

Keyword(s):

Essential Oils ◽

Batch Culture ◽

Methane Production ◽

Neutral Detergent Fiber ◽

Anethum Graveolens ◽

Acetate Concentration ◽

Screening Experiments ◽

Lavandula Latifolia

Tekippe, J. A., Hristov, A. N., Heyler, K. S., Zheljazkov, V. D., Ferreira, J. F. S., Cantrell, C. L. and Varga, G. A. 2012. Effects of plants and essential oils on ruminal in vitro batch culture methane production and fermentation. Can. J. Anim. Sci. 92: 395–408. In this study, plants (14) and essential oils (EO; 88) from plants that are naturalized to, or can be successfully grown in North America were evaluated in a batch culture in vitro screening experiments with ruminal fluid as potential anti-methanogenic additives for ruminant diets. Essential oils were tested at four inclusion levels: 0 (blank), 10, 50, and 100 mg L−1and plants were tested at 313, 1250, 2500, and 5000 mg L−1final incubation medium concentration. Compared with the blank, two of the EO increased acetate concentration (8 to 10%), 11 EO increased propionate concentration (9 to 23%), 10 EO increased butyrate concentration (24 to 29%), and three EO reduced methane production [20 to 30%; Anethum graveolens (dill weed oil), Lavandula latifolia, and Ocimum basilicum #7 accession]. Four EO decreased and one increased neutral detergent fiber (NDF) degradability. Three plants increased acetate concentration (8 to 12%), two increased propionate concentration (16%), and one (Origanum vulgare) decreased methane production (31%). Eight of the plants increased NDF degradability at various inclusion levels. Overall, these results indicate that some EO, or EO-producing plants could have a potential anti-methanogenic effect. Further research is needed to verify these results in vivo in long-term experiments.

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Influence of the Composition of the Cellulolytic Flora on the Development of Hydrogenotrophic Microorganisms, Hydrogen Utilization, and Methane Production in the Rumens of Gnotobiotically Reared Lambs

Applied and Environmental Microbiology ◽

10.1128/aem.01784-10 ◽

2010 ◽

Vol 76 (24) ◽

pp. 7931-7937 ◽

Cited By ~ 30

Author(s):

Frédérique Chaucheyras-Durand ◽

Sébastien Masséglia ◽

Gérard Fonty ◽

Evelyne Forano

Keyword(s):

Methane Production ◽

Bacterial Communities ◽

Rumen Content ◽

Fibrobacter Succinogenes ◽

Anaerobic Fungi ◽

Fiber Degradation ◽

Ruminal Microbiota ◽

Hydrogen Utilization

ABSTRACT We investigated the influence of the composition of the fibrolytic microbial community on the development and activities of hydrogen-utilizing microorganisms in the rumens of gnotobiotically reared lambs. Two groups of lambs were reared. The first group was inoculated with Fibrobacter succinogenes, a non-H2-producing species, as the main cellulolytic organism, and the second group was inoculated with Ruminococcus albus, Ruminococcus flavefaciens, and anaerobic fungi that produce hydrogen. The development of hydrogenotrophic bacterial communities, i.e., acetogens, fumarate and sulfate reducers, was monitored in the absence of methanogens and after inoculation of methanogens. Hydrogen production and utilization and methane production were measured in rumen content samples incubated in vitro in the presence of exogenous hydrogen (supplemented with fumarate or not supplemented with fumarate) or in the presence of ground alfalfa hay as a degradable substrate. Our results show that methane production was clearly reduced when the dominant fibrolytic species was a non-H2-producing species, such as Fibrobacter succinogenes, without significantly impairing fiber degradation and fermentations in the rumen. The addition of fumarate to the rumen contents stimulated H2 utilization only by the ruminal microbiota inoculated with F. succinogenes, suggesting that these communities could play an important role in fumarate reduction in vivo.

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Stoichiometry ofin vitrofermentation of pure substrates with particular emphasis on methane production, using the cumulative gas production technique

Proceedings of the British Society of Animal Science ◽

10.1017/s0308229600029998 ◽

1996 ◽

Vol 1996 ◽

pp. 24-24

Author(s):

Angela R. Moss ◽

Karen C. O'Callaghan

Keyword(s):

Methane Production ◽

Rumen Fluid ◽

Gas Production ◽

Production Technique

There is a need to be able to measure the methane producing potential of a range of feedstuffs under different rumen conditions in order to predict more accurately the amount of methane produced per animal.In vitrotechniques using rumen fluid as an innoculum can be considered as models ofin vivorumen digestion and have been applied to estimate digestibility of feedstuffs. Thein vitrogas production technique (Menkeet. al., 1979) can be used successfully to estimate this but there has been limited attempts to use it to estimate the methane producing potential of feedstuffs. The objective was to assess the potential of the technique to do this.Three pure substrates, glucose (G), pectin (P) and cellulose (C) (lg) were pre-wetted in 94ml of medium D (Theodorouet. al., 1994). inoculated with strained rumen fluid (from wether sheep. 10ml) and incubated without agitation for 72h at 39Â°C.

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