Inter- and intra-individual variability of total antioxidant capacity of bovine seminal plasma and relationships with sperm quality before and after cryopreservation

Aim of this study was to evaluate the effects of short-term (7 days) N-acetylcysteine (NAC) at 1,200 mg daily supplementation on muscle fatigue, maximal oxygen uptake (), total antioxidant capacity (TAC), lactate, creatine kinase (CK), and tumor necrotic factor-alpha (TNF-α). Twenty-nine sedentary men (13 controls; 16 in the supplement group) from a randomized control were included. At before and after supplementation, fatigue index (FI) was evaluated in the quadriceps muscle, and performed a graded exercise treadmill test to induce oxidative stress, and as a measure of . Blood samples were taken before exercise and 20 minutes after it at before and after supplementation, to determine TAC, CK, lactate, and TNF-αlevels. Results showed that FI and increased significantly in the supplement group. After exercise decreased the levels of TAC and increased lactate, CK, and TNF-αof both groups at before supplementation. After supplementation, lactate, CK, and TNF-αlevels significantly increased and TAC decreased after exercise in the control group. Whereas the TAC and lactate levels did not change significantly, but CK and TNF-αincreased significantly in the supplement group. Therefore, this results showed that NAC improved the muscle fatigue, , maintained TAC, controlled lactate production, but had no influence on CK and TNF-α.

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Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

Apidologie ◽

10.1007/s13592-015-0391-9 ◽

2015 ◽

Vol 47 (2) ◽

pp. 227-236 ◽

Cited By ~ 11

Author(s):

Mariola Słowińska ◽

Joanna Nynca ◽

Jerzy Wilde ◽

Beata Bąk ◽

Maciej Siuda ◽

...

Keyword(s):

Antioxidant Capacity ◽

Seminal Plasma ◽

Total Antioxidant Capacity ◽

Total Antioxidant

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Correlation of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Men With Idiopathic Infertility

Avicenna Journal of Medical Biochemistry ◽

10.17795/ajmb-29736 ◽

2016 ◽

Vol Inpress (Inpress) ◽

Cited By ~ 2

Author(s):

Faramarz Fazeli ◽

Saeedeh Salimi

Keyword(s):

Antioxidant Capacity ◽

Seminal Plasma ◽

Total Antioxidant Capacity ◽

Sperm Parameters ◽

Idiopathic Infertility ◽

Total Antioxidant

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Total antioxidant capacity, catalase activity, and lipid peroxidation changes in seminal plasma of sex-reversed female and male rainbow trout (Oncorhynchus mykiss) during spawning season

Theriogenology ◽

10.1016/j.theriogenology.2016.06.014 ◽

2016 ◽

Vol 86 (8) ◽

pp. 1975-1982 ◽

Cited By ~ 7

Author(s):

B.E. İnanan ◽

F. Öğretmen ◽

T. İnanan ◽

F. Yılmaz

Keyword(s):

Lipid Peroxidation ◽

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Antioxidant Capacity ◽

Seminal Plasma ◽

Catalase Activity ◽

Total Antioxidant Capacity ◽

Spawning Season ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Total Antioxidant

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Hormonal Regulation of Total Antioxidant Capacity in Seminal Plasma

Journal of Andrology ◽

10.2164/jandrol.108.006148 ◽

2009 ◽

Vol 30 (5) ◽

pp. 534-540 ◽

Cited By ~ 22

Author(s):

A. Mancini ◽

R. Festa ◽

A. Silvestrini ◽

N. Nicolotti ◽

V. Di Donna ◽

...

Keyword(s):

Antioxidant Capacity ◽

Seminal Plasma ◽

Total Antioxidant Capacity ◽

Hormonal Regulation ◽

Total Antioxidant

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266 ANTIOXIDANT CAPACITY OF BOAR SEMINAL PLASMA

Reproduction Fertility and Development ◽

10.1071/rdv17n2ab266 ◽

2005 ◽

Vol 17 (2) ◽

pp. 283 ◽

Cited By ~ 1

Author(s):

M. Hernandez ◽

A. Cano ◽

M.B. Arnao ◽

X. Lucas ◽

J.M. Vazquez ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Antioxidant Capacity ◽

Seminal Plasma ◽

Total Antioxidant Capacity ◽

Room Temperature ◽

Color Characteristics ◽

Nylon Mesh ◽

Total Antioxidant ◽

Lipophilic Antioxidant

It has been established that antioxidants in seminal plasma play an important role in protecting the spermatozoa against oxidative stress-induced damage. This study was conducted to measure the total antioxidant capacity (TAC) of boar seminal plasma. Fifty-four ejaculates were collected from 17 mature boars of proven fertility by the gloved-hand technique. Ejaculates were collected separately in different fractions (pre-sperm, sperm-rich, and post-sperm) according to their macroscopic (color) characteristics. After centrifugation (2400g for 3 min), the sperm pellet was discarded; the supernatant was recentrifuged and filtered through a 10-μm nylon mesh filter to remove debris or clumped spermatozoa. The seminal plasma was frozen at -20°C until further use. After thawing at room temperature, seminal plasma aliquots of 5 μL were immediately assessed for total antioxidant capacity. TAC was measured using the ABTS/H2O2/HRP decoloration method (Cano A et al. 2000 Redox Report 5, 365–370) which allows differentiation between hydrophilic and lipophilic antioxidant activity capacity. TAC units were expressed as micromolar (μM) Trolox equivalents. Data were analyzed using ANOVA. Only the hydrophilic activity was measurable, with the lipophilic activity being undetected. The overall TAC of seminal samples (mean ± SEM) was 1623.7 ± 56.28 μM, ranging from 674 to 2428 μM. Different TACs were observed among males (P < 0.05) and between ejaculates of the same male (P < 0.05). Ejaculate fraction had a significant effect (P < 0.001) on the TAC levels. The post-sperm fraction had a significantly lower TAC level (1104.09 ± 57.66 μM) than the pre-sperm and sperm-rich fractions (1611.95 ± 153.68 μM and 1356.136 ± 72.47 μM, respectively, P < 0.001). In conclusion, hydrophilic antioxidant activity represented the main contribution to the TAC in boar seminal plasma, showing differences among males, between ejaculates of the same male, and also between the different ejaculate fractions. This work was supported by CICYT (AGF98-0533; AGL01-0471) and INIA (RZ01-019).

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