Biofuel production from birch wood by combining high solid loading simultaneous saccharification and fermentation and anaerobic digestion

2017 ◽  
Vol 193 ◽  
pp. 210-219 ◽  
Author(s):  
Dayanand Chandrahas Kalyani ◽  
Mirzaman Zamanzadeh ◽  
Gerdt Müller ◽  
Svein J. Horn
Energies ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 2090
Author(s):  
Inês C. Roberto ◽  
Rafael C. A. Castro ◽  
João Paulo A. Silva ◽  
Solange I. Mussatto

Simultaneous saccharification and fermentation (SSF) at high solid loading is a potential approach to improve the economic feasibility of cellulosic ethanol. In this study, SSF using high loading of rice straw was assessed using a vertical ball mill reactor. First, the conditions of temperature and number of glass spheres were optimized at 8% (w/v) initial solids (41.5 °C, 18 spheres). Then, assays were carried out at higher solid loadings (16% and 24% w/v). At 8% or 16% solids, the fermentation efficiency was similar (ηF~75%), but the ethanol volumetric productivity (QP) reduced from 1.50 to 1.14 g/L.h. By increasing the solids to 24%, the process was strongly affected (ηF = 40% and QP = 0.7 g/L.h). To overcome this drawback, three different feeding profiles of 24% pre-treated rice straw were investigated. Gradual feeding of the substrate (initial load of 16% with additions of 4% at 10 and 24 h) and an inoculum level of 3 g/L resulted in a high ethanol titer (52.3 g/L) with QP of 1.1 g/L.h and ηF of 67%. These findings demonstrated that using a suitable fed-batch feeding strategy helps to overcome the limitations of SSF in batch mode caused by the use of high solid content.


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