Nitrocellulose Membrane for Paper-based Biosensor

2022 ◽  
Vol 26 ◽  
pp. 101305
Author(s):  
Ruihua Tang ◽  
Ming Yue Xie ◽  
Min Li ◽  
Lei Cao ◽  
Shangsheng Feng ◽  
...  
Keyword(s):  
Nitrocellulose Membrane

Amidolytic Detection of Prothrombin Activation Products after SDS-Gel Electrophoresis

1989 ◽  
Vol 61 (03) ◽  
pp. 386-391 ◽  
Author(s):  
Guido Tans ◽  
Truus Janssen-Claessen ◽  
Jan Rosing
Keyword(s):  
Gel Electrophoresis ◽  
Activated Protein C ◽  
Factor Xa ◽  
Coagulation Factors ◽  
Product Formation ◽  
Chromogenic Substrate ◽  
Nitrocellulose Membrane ◽  
Activation Products ◽  
Factor Xia ◽  
Prothrombin Activation

SummaryIn this paper we report a method via which enzymatically active products formed during prothrombin activation can be detected by simple photographic means after SDS-gel electrophoresis, blotting onto a nitrocellulose membrane and visualization with the chromogenic substrate, S2238. After amidolytic detection the same nitrocellulose membrane can also be used for immunologic detection of prothrombin activation products, thus allowing a complete description of product formation during prothrombin activation.The detection limit of the so-called “amidoblot” is approximately 3 ng thrombin per gel sample which is comparable to the sensitivity of immunoblotting.It is further shown that the amidoblot technique can also be applied to other coagulation factors for which a suitable chromogenic substrate is available (factor XIIa, kallikrein, factor XIa, factor Xa, plasmin and activated protein C).

scholarly journals Evolution of antibody response and fungal antigens in the serum of a patient infected with Candida famata

2007 ◽  
Vol 56 (5) ◽  
pp. 571-578 ◽  
Author(s):  
Diana Pisa ◽  
Marta Ramos ◽  
Susana Molina ◽  
Patricia García ◽  
Luis Carrasco
Keyword(s):  
Antibody Response ◽  
Antifungal Treatment ◽  
Antifungal Compounds ◽  
Sensitive Test ◽  
Nitrocellulose Membrane ◽  
Immunofluorescence Analysis ◽  
Candida Famata ◽  
Fungal Antigens

The presence of fungal antibodies and antigens in the serum of a patient diagnosed in 1996 with acute zonal occult outer retinopathy caused by Candida famata infection was examined. Antibodies against C. famata increased until 1999–2000 when antifungal treatment was initiated. The antibodies were detected by ELISA and immunofluorescence analysis using C. famata. These antibodies were not immunoreactive against several Candida species tested. Positive immunofluorescence was obtained with IgM, but not IgA, IgG or IgE. Moreover, the IgM response disappeared several months after treatment with antifungal compounds, despite the fact that C. famata antigens were present in the blood. Finally, a sensitive test was developed to assay for the presence of C. famata antigens in serum based on the immunodetection of fungal antigens transferred to a nitrocellulose membrane and incubated with rabbit antibodies raised against C. famata. According to this method, the infection diminished with antifungal treatment.

scholarly journals Glycerol Reduces Cross Hybridization on Nitrocellulose Membrane

2020 ◽  
Vol 38 (3) ◽  
pp. 199
Author(s):  
Narendra Yoga Hendarta ◽  
Abu Tholib Aman ◽  
Asmarani Kusumawati ◽  
Tri Wibawa
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Gc Content ◽  
Lateral Flow ◽  
Hybridization Signal ◽  
Nitrocellulose Membrane ◽  
High Concentration ◽  
Cross Hybridization ◽  
High Stringency ◽  
Stringency Condition

Lateral flow assay (LFD) based nucleic acid lateral flow (NALF)  method has been developed recently. The method met point of care testing (POCT) as simple and rapid procedures, less equipment, and can be performance by less skilled technician. NALF based on nucleic acid hybridizationis  more economical then immunochromatography assay which use antibody-antigen recognition. Cross hybridization has issued while used to differentiate organism with high GC content and high homology as high similarity genome. Some techniques has applied to give high stringency condition avoid cross hybridization reaction but need more procedure to apply. We found glycerol applied to buffer assay could reduce cross hybridization on nitrocellulose membrane. The study used 2 kinds of high stringency buffer as PBS and SSC bases and high concentration of ssDNA amplicon as sample. Without glycerol ingredient gave cross hybridization signal on test line. But used glycerol could reduce those even omitted with PBS based buffer assay. Beside those, glycerol could significantly increased hybridization signal in SSC based buffer assay (p<0.05).

Hybridoma Screening by Antigen Capture: Reverse Dot Blot

2022 ◽  
Vol 2022 (1) ◽  
pp. pdb.prot103135
Author(s):  
Edward A. Greenfield
Keyword(s):  
Tissue Culture ◽  
Monoclonal Antibodies ◽  
Culture Supernatant ◽  
Membrane Surface ◽  
Nitrocellulose Membrane ◽  
Dot Blot ◽  
Tissue Culture Supernatant ◽  
Antigen Capture ◽  
Mouse Immunoglobulin ◽  
Reverse Dot Blot

A dot blot is widely used to determine the productivity of a given hybridoma. This assay can also be used to screen a fusion or subclone plate for productive hybridoma clones. First, a nitrocellulose membrane is coated with an affinity-purified goat or rabbit anti-mouse immunoglobulin and then incubated with hybridoma tissue culture supernatant. Monoclonal antibodies in the supernatant are then “captured” on the coated nitrocellulose membrane surface and detected by screening with horseradish peroxidase (HRP).

"Microgel diffusion blotting" for sensitive detection of antibodies to extractable nuclear antigens

1990 ◽  
Vol 36 (2) ◽  
pp. 337-339 ◽  
Author(s):  
F De Keyser ◽  
G Verbruggen ◽  
E M Veys ◽  
J Nimmegeers ◽  
L Schatteman ◽  
...  
Keyword(s):  
Rheumatic Diseases ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Sensitive Detection ◽  
Nitrocellulose Membrane ◽  
Double Immunodiffusion ◽  
Simple Diffusion ◽  
Gel Layer ◽  
Nuclear Antigens

Abstract A fast immunoblotting procedure, termed "microgel diffusion blotting," is used to detect and identify antibodies to extractable nuclear antigens (i.e., to Sm, RNP, and SSB) in patients with rheumatic diseases. The method differs from the standard immunoblotting techniques by the use of ultra-thin microgels for polyacrylamide gel electrophoresis: the very thin gel layer allows transfer of proteins to a nitrocellulose membrane by simple diffusion. Principal advantages of this variant technique are its simplicity, rapidity, and reproducibility--characteristics that make the test suitable for routine application. We compared the sensitivity of the test with that of double immunodiffusion in agarose for the evaluation of humoral antinuclear immunity. Microgel diffusion blotting detected antibodies in serum at concentrations less than 0.001 of those detectable by immunodiffusion.

Differential Thick-Film Amperometric Glucose Sensor with an Enzyme-Immobilized Nitrocellulose Membrane

2001 ◽  
Vol 13 (3) ◽  
pp. 224-228 ◽  
Author(s):  
Gang Cui ◽  
Jae Hyun Yoo ◽  
Jina Yoo ◽  
Sang Woo Lee ◽  
Hakhyun Nam ◽  
...  
Keyword(s):  
Thick Film ◽  
Glucose Sensor ◽  
Nitrocellulose Membrane ◽  
Amperometric Glucose Sensor

A nitrocellulose membrane-based integrated microfluidic system for bacterial detection utilizing magnetic-composite membrane microdevices and bacteria-specific aptamers

Lab on a Chip ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 1633-1640 ◽  
Author(s):  
Jia-Han Wu ◽  
Chih-Hung Wang ◽  
Yu-Dong Ma ◽  
Gwo-Bin Lee
Keyword(s):  
Acinetobacter Baumannii ◽  
Composite Membrane ◽  
Microfluidic System ◽  
Magnetic Composite ◽  
Nitrocellulose Membrane ◽  
Bacterial Detection ◽  
Detection Process

A new, nitrocellulose-based microfluidic system featuring Acinetobacter baumannii (AB)-specific aptamers capable of automating the bacterial detection process was reported.

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