Dissolution of minerals during hydrolysis of fish waste solids

Gelatin extracted from fish skin was used as an expedient substrate and a vital source of protein to assess the efficient production of gelatinase by Serratia marcescens MF599353 over hydrolysis of the gelatin. During the growth of the organism, a pigment called prodigiosin was produced, and its molecular weight was determined to be 324Da by ESI-MS and further characterized by FTIR. Using the OFT (one-factor at-a-time) approach, various parameters such as time, pH, temperature, gelatin, and yeast extract concentration were optimized for gelatinase activity. The molecular weight of purified gelatinase was determined to be 44 kDa using SDS-PAGE. Thus, the cultivation of Serratia marcescens MF599353 under optimized conditions could enhance the gelatinase production and its potential applicability at industrial scale.

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Enzymatic Hydrolysis of Fish Waste as an Alternative to Produce High Value-Added Products

Waste and Biomass Valorization ◽

10.1007/s12649-020-01029-x ◽

2020 ◽

Cited By ~ 2

Author(s):

J. Araujo ◽

P. Sica ◽

C. Costa ◽

M. C. Márquez

Keyword(s):

Enzymatic Hydrolysis ◽

Value Added ◽

Fish Waste ◽

Hydrolysis Of ◽

Value Added Products

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OPTIMIZATION RATIO ENZIM PAPAIN AND BOMELIN ON PROCESS OF MAKING SOLID ORGANIC FERTILIZER

10.31227/osf.io/dh8w9 ◽

2018 ◽

Author(s):

syafrani

Keyword(s):

Negative Impact ◽

Organic Fertilizer ◽

Economic Value ◽

Market Area ◽

Design Factor ◽

Fish Waste ◽

Fermentation Time ◽

Randomized Design ◽

Hydrolysis Of ◽

Enzyme Ratio

Increasing of population needed for clothing and food impacts to the environment such as waste, from a business or activity such as industry, market area, household and other activities. Waste generated by various businesses and activities in terms of both organic and inorganic chemistry, with the quantity and content of chemistry, which can have a negative impact on the environment, one source of such waste is from the fish market. To cope the fish maket waste in order to have economic value need to adopted a concept 3R ( reduce, reuse, and recycle) and take advantage of fish by-products into economic products. This study has two purposes, general and specific goals. The objective of the study was to obtain the optimal feasibility level of the best enzyme and bromeline conservation ratio in the hydrolysis of fish waste into solid organic fertilizer. Using Completely Randomized Design. Factor A is a combination of papain-bromelin enzyme, which comprises 0%. 2% (1: 3), 4% (1: 1) and 6% (3: 1), while factor B is fermentation length, 0 days, 3 days, 6 days and 9 days, with 3 repetitions. The parameters observed from this experiment were, pH and CNPK performed in accordance with fermentation time. The results showed the ratio of interaction of 1: 1 papain-bromelin enzyme ratio with 4% concentration and 3-day fermentation length (E4H3) of sample weight showed the best result compared to other treatments yielding C-organic 9.52%, nitrogen 1, 32% , 0.30% phosphate, and potassium by 0.14%, and final pH of the study 5.58.

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SYNTHESIS AND ANALYSIS OF COPPER PROTEINATE AND MANGANESE PROTEINATE FROM REACTION OF COPPER SULFATE AND MANGANESE SULFATE WITH PROTEIN EXTRACTED FROM FISH WASTE

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020.v12s1.ff031 ◽

2020 ◽

pp. 139-142

Author(s):

HARMITA HARMITA ◽

DANNIS SAMUEL SIMBOLON

Keyword(s):

Atomic Absorption Spectrophotometry ◽

Exchange Chromatography ◽

The Body ◽

Free Form ◽

Manganese Sulfate ◽

Biological Processes ◽

Fish Waste ◽

Essential Minerals ◽

Hydrolysis Of ◽

Free Metal

Objective: Copper and manganese are essential minerals needed for various biological processes in small amounts. However, essential mineralsare poorly absorbed in the form of salts or free form, leading to their low bioavailability. Forming complexes of essential minerals with protein canincrease their bioavailability. Metal proteinate complexes are non-polar, thereby reducing their excretion from the body. Fish waste is quite abundantin Indonesia, and therefore, we used fish waste to synthesize metal-proteinate complexes.Methods: Protein was extracted from fish waste using pancreatin. The extracted protein was mixed with copper or manganese in various ratios. Themetal content in the complexes was analyzed using atomic absorption spectrophotometry; ion exchange chromatography was used for separating thecomplexes from free unbound metals.Results: The optimum condition which yielded the highest protein content was the ratio of pancreatin enzyme to fish waste powder of 2:100. Theoptimum concentration of pancreatin was found to be 2% of the substrate. The yield of copper-proteinate complexes ranged from 97.87% to 98.55%,whereas the yield of manganese proteinate ranged from 97.05% to 98.36%. The free metal content was only found in the manganese proteinatecomplex in the 1.2:1 ratio, which was determined to be 0.0198 mg/g.Conclusion: We demonstrated that copper and manganese can react with proteins extracted by enzymatic hydrolysis of fish waste.

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Fine Structural Localization of Acyltransferases Involved in Lipid Synthesis in Intestinal Mucosa.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100067261 ◽

1970 ◽

Vol 28 ◽

pp. 54-55

Author(s):

R. J. Barrnett ◽

J. A. Higgins

Keyword(s):

Smooth Endoplasmic Reticulum ◽

Lipid Synthesis ◽

Mucosal Cell ◽

Structural Localization ◽

Morphological Studies ◽

Mucosal Cells ◽

Initial Appearance ◽

Sh Group ◽

Hydrolysis Of ◽

Intestinal Mucosal Cells

The main products of intestinal hydrolysis of dietary triglycerides are free fatty acids and monoglycerides. These form micelles from which the lipids are absorbed across the mucosal cell brush border. Biochemical studies have indicated that intestinal mucosal cells possess a triglyceride synthesising system, which uses monoglyceride directly as an acylacceptor as well as the system found in other tissues in which alphaglycerophosphate is the acylacceptor. The former pathway is used preferentially for the resynthesis of triglyceride from absorbed lipid, while the latter is used mainly for phospholipid synthesis. Both lipids are incorporated into chylomicrons. Morphological studies have shown that during fat absorption there is an initial appearance of fat droplets within the cisternae of the smooth endoplasmic reticulum and that these subsequently accumulate in the golgi elements from which they are released at the lateral borders of the cell as chylomicrons.We have recently developed several methods for the fine structural localization of acyltransferases dependent on the precipitation, in an electron dense form, of CoA released during the transfer of the acyl group to an acceptor, and have now applied these methods to a study of the fine structural localization of the enzymes involved in chylomicron lipid biosynthesis. These methods are based on the reduction of ferricyanide ions by the free SH group of CoA.

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Lattice imaging and pore structure of β ferric oxyhydroxide

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100108465 ◽

1978 ◽

Vol 36 (1) ◽

pp. 264-265

Author(s):

T. Baird ◽

J.R. Fryer ◽

S.T. Galbraith

Keyword(s):

Electron Microscopy ◽

Room Temperature ◽

Bulk Material ◽

Model Structure ◽

Bulk Crystals ◽

Lattice Imaging ◽

Thin Sections ◽

Ferric Oxyhydroxide ◽

Resolution Electron ◽

Hydrolysis Of

Introduction Previously we had suggested (l) that the striations observed in the pod shaped crystals of β FeOOH were an artefact of imaging in the electron microscope. Contrary to this adsorption measurements on bulk material had indicated the presence of some porosity and Gallagher (2) had proposed a model structure - based on the hollandite structure - showing the hollandite rods forming the sides of 30Å pores running the length of the crystal. Low resolution electron microscopy by Watson (3) on sectioned crystals embedded in methylmethacrylate had tended to support the existence of such pores.We have applied modern high resolution techniques to the bulk crystals and thin sections of them without confirming these earlier postulatesExperimental β FeOOH was prepared by room temperature hydrolysis of 0.01M solutions of FeCl3.6H2O, The precipitate was washed, dried in air, and embedded in Scandiplast resin. The sections were out on an LKB III Ultramicrotome to a thickness of about 500Å.

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