Genetic parameters for resistance to Aeromonas hydrophila in the Neotropical fish pacu (Piaractus mesopotamicus)

Aquaculture ◽  
2019 ◽  
Vol 513 ◽  
pp. 734442 ◽  
Author(s):  
Vito A. Mastrochirico-Filho ◽  
Raquel B. Ariede ◽  
Milena V. Freitas ◽  
Lieschen V.G. Lira ◽  
John F.G. Agudelo ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Genetic Parameters ◽  
Neotropical Fish ◽  
Piaractus Mesopotamicus

Genotype by environment interaction and genetic parameters for growth traits in the Neotropical fish pacu (Piaractus mesopotamicus)

Aquaculture ◽  
2021 ◽  
Vol 530 ◽  
pp. 735933
Author(s):  
Milena V. Freitas ◽  
Lieschen V.G. Lira ◽  
Raquel B. Ariede ◽  
John F.G. Agudelo ◽  
Rubens Ricardo de Oliveira Neto ◽  
...  
Keyword(s):  
Genetic Parameters ◽  
Growth Traits ◽  
Genotype By Environment Interaction ◽  
Environment Interaction ◽  
Neotropical Fish ◽  
Piaractus Mesopotamicus ◽  
Genotype By Environment

scholarly journals Transferability of heterologous microsatellite primers in Brycon gouldingi

2018 ◽  
Vol 48 (11) ◽  
Author(s):  
Felipe Pinheiro de Souza ◽  
Ed Christian Suzuki de Lima ◽  
Natalia Gonçalves Leite ◽  
Angela Maria Urrea-Rojas ◽  
Andrei Lincoln Yamachita ◽  
...  
Keyword(s):  
Genetic Parameters ◽  
Null Alleles ◽  
Microsatellite Primers ◽  
Neotropical Fish ◽  
Genetic Studies ◽  
Prochilodus Lineatus ◽  
Colossoma Macropomum ◽  
Piaractus Mesopotamicus ◽  
Future Studies ◽  
Environmental Importance

ABSTRACT: Brycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were assessed. Among the 30 primers, seven showed satisfactory transferability, six of which belonged to the genus Brycon: Bh13 (B. hilarii), BoM5, BoM13 (B. opalinus), Borg9, Borg13, and Borg59 (B. orbignyanus), and one belonged to P. argenteus (Par80). The primers for the other species tested showed non-specificity or monomorphism; and were therefore, excluded from the analyses. The number of alleles ranged between two (Borg13 and Borg59) and three (Bh13, BoM5, BoM13, Borg9 and Par80), with sizes varying between 103 bp (BoM5) and 430 bp (Borg9). Four primers showed evidence of null alleles (BoM13, Borg9, Borg13, and Par80), which could probably be attributed to the respective Hardy-Weinberg deviation. Thus, seven primers were validated for cross-amplification in B. gouldingi, which may be used in future studies involving this species.

Dietary intake of Rubrivivax gelatinosus biomass enhances phagocytic cells in tropical fish Piaractus mesopotamicus infected with Aeromonas hydrophila

2019 ◽  
Vol 27 (3) ◽  
pp. 711-720
Author(s):  
S. U. Gallani ◽  
G. M. R. Valladão ◽  
S. Kotzent ◽  
E. F. E. Santo ◽  
E. H. G. Ponsano ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Aeromonas Hydrophila ◽  
Tropical Fish ◽  
Phagocytic Cells ◽  
Piaractus Mesopotamicus ◽  
Rubrivivax Gelatinosus

Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila

Aquaculture ◽  
2020 ◽  
Vol 523 ◽  
pp. 735231 ◽  
Author(s):  
Victor Alexander Cueva-Quiroz ◽  
Jefferson Yunis-Aguinaga ◽  
Fernando Carlos Ramos-Espinoza ◽  
Flávio Ruas de Moraes ◽  
Julieta Rodini Engrácia de Moraes
Keyword(s):  
Aeromonas Hydrophila ◽  
Innate Immune ◽  
Piaractus Mesopotamicus ◽  
Immune Parameters

scholarly journals Cross-amplification of heterologous microsatellite markers in Piracanjuba

2017 ◽  
Vol 47 (12) ◽  
Author(s):  
Pedro Luiz de Castro ◽  
Ricardo Pereira Ribeiro ◽  
Silvio Carlos Alves dos Santos ◽  
Elenice Souza dos Reis Goes ◽  
Felipe Pinheiro de Souza ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Microsatellite Markers ◽  
Oreochromis Niloticus ◽  
Genetic Parameters ◽  
Economic Value ◽  
Current Analysis ◽  
Genetic Studies ◽  
Colossoma Macropomum ◽  
Piaractus Mesopotamicus ◽  
Cross Amplification

ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.

Growth performance and metabolism of the Neotropical fish Piaractus mesopotamicus under sustained swimming

Aquaculture ◽  
2019 ◽  
Vol 511 ◽  
pp. 734219
Author(s):  
Luis Antonio Kioshi Aoki Inoue ◽  
Araceli Hackbarth ◽  
Gustavo Arberláez-Rojas ◽  
Gilberto Moraes
Keyword(s):  
Growth Performance ◽  
Neotropical Fish ◽  
Piaractus Mesopotamicus

scholarly journals The basis of vagal efferent control of heart rate in a neotropical fish, the pacu, Piaractus mesopotamicus

2009 ◽  
Vol 212 (7) ◽  
pp. 906-913 ◽  
Author(s):  
E. W. Taylor ◽  
C. A. C. Leite ◽  
L. H. Florindo ◽  
T. Belao ◽  
F. T. Rantin
Keyword(s):  
Heart Rate ◽  
Neotropical Fish ◽  
Piaractus Mesopotamicus ◽  
Efferent Control

scholarly journals ALTERAÇÕES MORFOLÓGICAS DE LEUCÓCITOS E TROMBÓCITOS SANGUÍNEOS EM Piaractus mesopotamicus DURANTE A SEPSE

2020 ◽  
Vol 11 (2) ◽  
pp. 18
Author(s):  
Vivian Cristian de Freitas Lima ◽  
Andreia S Oliveira ◽  
Débora K.T. Moreira ◽  
Alanna S.L Silva ◽  
Alberto C. F. Da Silva ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Piaractus Mesopotamicus

A sepse é definida como disfunção orgânica com alta mortalidade. Durante esse processo o sistema hematológico desempenha um papel crítico. Assim, o trabalho objetivou analisar as alterações morfológicas no leucograma e no trombograma pelo método indireto no curso da sepse em Piaractus mesopotamicus. Para tanto, foram utilizados 98 pacus, divididos em dois grupos, um deles recebeu 0,5 ml de solução de cloreto de sódio esterilizada a 0,65% (controle) e outro recebeu o mesmo volume contendo o inoculo bacteriano (18 x 108 CFU/ml – desafio). Para avaliar tais alterações na evolução da sepse foram realizadas coletas de sangue dos peixes 1, 3, 6 e 9 horas após inoculação e no grupo controle (n=10). Uma alíquota do sangue foi destina a hemocultura e outra na determinação do número de leucócitos e de trombóticos pelo método indireto e a avaliação das alterações morfológicas nas extensões sanguíneas. A aeromonose foi confirmada pela hemocultura positiva em todas as amostras dos grupos desafiados. A análise do leucograma demonstrou aumento significativa (p<0,05) dos leucócitos nos tempos mais tardios, 9 e 6 HPI em relação ao grupo controle. Da análise diferencial observou-se leucopenia (p<0,05; 3 HPI) e consumo dos trombócitos. Verificou-se que com o aumento da infecção os pacus septicêmicos desenvolvem um quadro de granulocitose e monocitose (p<0,05). Concluiu-se que a inoculação com A. hydrophila induziu a sepse com hemocultura positiva com leucocitose nos pacus desafiados e causa intensa alteração na morfologia dos leucócitos e trombóticos.PALAVRAS-CHAVE: Hemocultura, Leucograma, Morfologia, Aeromonas hydrophila, Teleósteos.

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