Morphological changes of pyloric caeca and their relevancy to motility in laboratory-reared Kurosoi rockfish (Sebastes schlegelii Hilgendorf), using an in vitro assay method

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

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AN IN VITRO ASSAY OF CORTICOTROPHIN

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y55-030 ◽

1955 ◽

Vol 33 (1) ◽

pp. 209-216

Author(s):

K. W. McKerns ◽

E. Nordstrand

Keyword(s):

Dose Response ◽

In Vitro Assay ◽

Assay Method ◽

Response Curves ◽

Vitro Assay ◽

Rat Adrenals ◽

Assay Design ◽

Dose Response Curves ◽

Gland System

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

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An In Vitro Assay Method for Resistance to Bacterial Wilt (Ralstonia solanacearum) in Potato

American Journal of Potato Research ◽

10.1007/s12230-018-9643-3 ◽

2018 ◽

Vol 95 (3) ◽

pp. 311-316 ◽

Cited By ~ 2

Author(s):

Ippei Habe

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

In Vitro Assay ◽

Assay Method ◽

Vitro Assay

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Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits

Food Chemistry ◽

10.1016/j.foodchem.2013.12.034 ◽

2014 ◽

Vol 153 ◽

pp. 130-133 ◽

Cited By ~ 11

Author(s):

Gema Flores ◽

Fernando De la Peña Moreno ◽

Gracia Patricia Blanch ◽

Maria Luisa Ruiz del Castillo

Keyword(s):

Enzyme Activity ◽

Phenylalanine Ammonia Lyase ◽

In Vitro Assay ◽

Assay Method ◽

Vitro Assay ◽

Flavonol Synthase ◽

Berry Fruits

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Development of a Highly Sensitive In Vitro Assay Method for Biological Activity of Endotoxin Contamination in Biological Products

Biologicals ◽

10.1006/biol.2002.0323 ◽

2002 ◽

Vol 30 (2) ◽

pp. 85-92 ◽

Cited By ~ 7

Author(s):

Akihiko Yamamoto ◽

Masaki Ochiai ◽

Michiyo Kataoka ◽

Hiromi Toyoizumi ◽

Yoshinobu Horiuchi

Keyword(s):

Biological Activity ◽

In Vitro Assay ◽

Assay Method ◽

Vitro Assay ◽

Biological Products ◽

Highly Sensitive ◽

Endotoxin Contamination

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EFFECTS OF INCUBATION CONDITIONS ON THE IN VITRO ASSAY METHOD FOR INSULIN

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o61-147 ◽

1961 ◽

Vol 39 (9) ◽

pp. 1381-1387 ◽

Cited By ~ 2

Author(s):

D. C. Jessup ◽

G. S. Wiberg

Keyword(s):

Adverse Effect ◽

Incubation Period ◽

Incubation Time ◽

Glucose Concentration ◽

In Vitro Assay ◽

Assay Method ◽

Vitro Assay ◽

Number Of Factors

A number of factors such as incubation time, glucose concentration, and preincubation cooling affect the deposition of glycogen and the uptake of glucose by diaphragm sections in response to insulin. The greatest response occurs during the first 30 minutes of incubation but for assay purposes an incubation period of 90 minutes was recommended as it gave the greatest difference between control and treated tissues. A glucose concentration of 0.2% in the medium was found optimum. The effect of insulin at higher and lower concentrations was not as great. Preincubation cooling had an adverse effect on the response of the tissues to insulin.

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THE BIOASSAY OF LONG-ACTING THYROID STIMULATOR: A COMPARISON OF TWO METHODS

Journal of Endocrinology ◽

10.1677/joe.0.0490487 ◽

1971 ◽

Vol 49 (3) ◽

pp. 487-492 ◽

Cited By ~ 4

Author(s):

JANICE M. ENSOR ◽

PAT KENDALL-TAYLOR ◽

D. S. MUNRO ◽

B. R. SMITH

Keyword(s):

Thyroid Stimulating Hormone ◽

In Vitro Assay ◽

Assay Method ◽

Vitro Assay ◽

In Vitro Method ◽

Long Acting ◽

Stimulating Hormone

SUMMARY The McKenzie (1958) assay method and an in-vitro assay method (Brown & Munro, 1967) for the determination of long-acting thyroid stimulator have been compared. Differences in the results were not great: the in-vitro method is simpler to perform and more precise. The sensitivity of the two methods is similar. The in-vitro method, when used with antiserum to thyroid-stimulating hormone in the medium, provided a satisfactory and simple means for the assay of long-acting thyroid stimulator in the serum of thyrotoxic patients.

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