Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits

2014 ◽  
Vol 153 ◽  
pp. 130-133 ◽  
Author(s):  
Gema Flores ◽  
Fernando De la Peña Moreno ◽  
Gracia Patricia Blanch ◽  
Maria Luisa Ruiz del Castillo
Keyword(s):  
Enzyme Activity ◽  
Phenylalanine Ammonia Lyase ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Flavonol Synthase ◽  
Berry Fruits

scholarly journals A fast, miniaturised in-vitro assay developed for quantification of lipase enzyme activity

2019 ◽  
Vol 34 (1) ◽  
pp. 1474-1480 ◽  
Author(s):  
Ariane Menden ◽  
Davane Hall ◽  
Daniel Paris ◽  
Venkatarian Mathura ◽  
Fiona Crawford ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Lipase Enzyme

AN IN VITRO ASSAY OF CORTICOTROPHIN

1955 ◽  
Vol 33 (2) ◽  
pp. 209-216 ◽  
Author(s):  
K. W. McKerns ◽  
E. Nordstrand
Keyword(s):  
Dose Response ◽  
In Vitro Assay ◽  
Assay Method ◽  
Response Curves ◽  
Vitro Assay ◽  
Rat Adrenals ◽  
Assay Design ◽  
Dose Response Curves ◽  
Gland System

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

AN IN VITRO ASSAY OF CORTICOTROPHIN

1955 ◽  
Vol 33 (1) ◽  
pp. 209-216
Author(s):  
K. W. McKerns ◽  
E. Nordstrand
Keyword(s):  
Dose Response ◽  
In Vitro Assay ◽  
Assay Method ◽  
Response Curves ◽  
Vitro Assay ◽  
Rat Adrenals ◽  
Assay Design ◽  
Dose Response Curves ◽  
Gland System

The ability of corticotrophin to increase the corticoid output of rat adrenals in an isolated gland system has been developed as a useful assay method for the measurement of corticotrophin potency. The extra corticoids produced by stimulation are measured in terms of cortisone. Log dose response curves are presented for corticotrophin levels from 0.002 to 0.135 I.U./100 mgm. adrenals. A four point assay design, the precision of corticoid measurements, and the characteristics of the log dose response curves for a number of types of corticotrophin are given. With four measurements of each dose level the average lambda s/b for 20 assays was 0.209 ± 0.085 (S.D.).

Investigations on the effects of hyperbaric oxygen on enzyme activity

1969 ◽  
Vol 47 (4) ◽  
pp. 400-402 ◽  
Author(s):  
Nicole Bégin-Heick ◽  
P. Hochstein ◽  
Gale B. Hill
Keyword(s):  
Enzyme Activity ◽  
Hyperbaric Oxygen ◽  
Enzyme Activities ◽  
Liver Tissue ◽  
In Vitro Assay ◽  
Vitro Assay

The ability of hyperbaric oxygen to produce lasting effects on enzyme activities was investigated. Mice were subjected to hyperbaric oxygen until they suffered sustained convulsions. The enzyme activities were then determined in brain and liver tissue. The results presented indicate that under these conditions of in vivo exposure and in vitro assay, hyperbaric oxygen does not affect permanently the activities of the enzymes which were examined.

scholarly journals PTP1B INHIBITORS FROM ISODON TERNIFOLIUS COLLECTED IN VIETNAM

2020 ◽  
Vol 58 (5) ◽  
pp. 533
Author(s):  
Nguyen Phi-Hung
Keyword(s):  
Enzyme Activity ◽  
Inhibitory Activity ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Chemical Structures ◽  
Whole Plant ◽  
Ic50 Values ◽  
Ptp1b Inhibitors ◽  
First Time

From the whole plant of Isodon ternifolius collected in Vietnam, four triterpens including ursaldehyde (1), ursolic acid (2), b-sitosterol (3) and b-sitosteryl ferulate (4) were purified. Their chemical structures were determined by interpretation of NMR and MS data and comparison with the literatures. Compounds 1-4 were evaluated for their inhibitory activity against PTP1B enzyme activity using in vitro assay. Compounds 1 and 2 displayed potential activities with IC50 values of 16.92 ± 0.12 and 3.42 ± 0.45 μM, respectively. This is the first time that compounds 1 and 4 have been isolated from the Isodon genus and I. ternifolius has been evaluated for the PTP1B inhibitory activity.

Development of a Highly Sensitive In Vitro Assay Method for Biological Activity of Endotoxin Contamination in Biological Products

Biologicals ◽  
2002 ◽  
Vol 30 (2) ◽  
pp. 85-92 ◽  
Author(s):  
Akihiko Yamamoto ◽  
Masaki Ochiai ◽  
Michiyo Kataoka ◽  
Hiromi Toyoizumi ◽  
Yoshinobu Horiuchi
Keyword(s):  
Biological Activity ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Biological Products ◽  
Highly Sensitive ◽  
Endotoxin Contamination

EFFECTS OF INCUBATION CONDITIONS ON THE IN VITRO ASSAY METHOD FOR INSULIN

1961 ◽  
Vol 39 (9) ◽  
pp. 1381-1387 ◽  
Author(s):  
D. C. Jessup ◽  
G. S. Wiberg
Keyword(s):  
Adverse Effect ◽  
Incubation Period ◽  
Incubation Time ◽  
Glucose Concentration ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
Number Of Factors

A number of factors such as incubation time, glucose concentration, and preincubation cooling affect the deposition of glycogen and the uptake of glucose by diaphragm sections in response to insulin. The greatest response occurs during the first 30 minutes of incubation but for assay purposes an incubation period of 90 minutes was recommended as it gave the greatest difference between control and treated tissues. A glucose concentration of 0.2% in the medium was found optimum. The effect of insulin at higher and lower concentrations was not as great. Preincubation cooling had an adverse effect on the response of the tissues to insulin.

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